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Written by the leading experts in the field, this book describes the development and current state of the art in single molecule spectroscopy. The application of this technique, which started 1989, in physics, chemistry and biosciences is displayed.
First multi-year cumulation covers six years: 1965-70.
This is the first book-length treatment of both the theoretical background to fluorescence correlation spectroscopy (FCS) and a variety of applications in various fields of science. The high spatial and temporal resolution of FCS has made it a powerful tool for the analysis of molecular interactions and kinetics, transport properties due to thermal motion, and flow. It contains an essential contribution from Nobel Prize winner M. Eigen, who is credited with inventing FCS.
The topics range from single molecule experiments in quantum optics and solid-state physics to analogous investigations in physical chemistry and biophysics.
th This proceedings volume of the 8 International Microchemical Symposium contains the plenary and keynote lectures delivered at the conference. Besides basic and historic aspects the following major topics are covered: "Microchemistry Arts and Archeology" in "Microchemistry in Life Sciences" "Microchemistry Sciences" in Environmental "Microchemistry in Material Sciences" "Instrumentation, Methods and Automation in Microchemistry". The papers show the present state of microchemistry and the development of this field since the pioneer days of Fritz Pregl and Friedrich Emich. Today microchemistry is a different science as compared to the Pregl and Emich days, for it combines many disciplines like chemistry, physics, mathematics, informatics, biology and does not only mean microanalysi- even if it is still predominant and the best tool for elucidation of the microcosmos. Due to this development modern microchemistry plays an important role in science and technology. It had been the intention of the Scientific th Executive Committee to demonstrate this at the 8 International Micro chemical Symposium with the goal to encourage interdisciplinary communication and stimulate discussion.
"Provides analytical chemists and biomedical scientists with an excellent summary of progress...This is a book that can be recommended to all analytical scientists interested in fluorimetry." (Analytical Chimica Acta) "This is a useful overview and gives the nonspecialist a feeling for the advantages and limitations of the methods. Overall this book is a worthwhile read and a good source of references." (TRAC) The book is divided into chapters on new methods, new appli- cations, fluorescence immunoassays, fluorometric analysis and fluorescence spectroscopy in biomedical sciences. Specific topics are fluorescence spectroscopy using synchrotron radiation, picosecond fluorescence spectroscopy, fluorescence microscopy, fluorescence scattering by synthetic polymers, fluorescence immunoassays, fluorescence for environmental monitoring, fluorescence in flow injection analysis, hydro-geological studies, fluorescence of proteins, lipids and membranes, cell fluorescence, calcium transients.
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This book presents a vivid argument for the almost lost idea of a unity of all natural sciences. It starts with the "strange" physics of matter, including particle physics, atomic physics and quantum mechanics, cosmology, relativity and their consequences (Chapter I), and it continues by describing the properties of material systems that are best understood by statistical and phase-space concepts (Chapter II). These lead to entropy and to the classical picture of quantitative information, initially devoid of value and meaning (Chapter III). Finally, "information space" and dynamics within it are introduced as a basis for semantics (Chapter IV), leading to an exploration of life and thought as new problems in physics (Chapter V). Dynamic equations - again of a strange (but very general) nature - bring about the complex familiarity of the world we live in. Surprising new results in the life sciences open our eyes to the richness of physical thought, and they show us what can and what cannot be explained by a Darwinian approach. The abstract physical approach is applicable to the origins of life, of meaningful information and even of our universe.