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Fluorescent Protein-Based Biosensors
In Fluorescent Protein-Based Biosensors: Methods and Protocols, experts in the field have assembled a series of protocols describing several methods in which fluorescent protein-based reporters can be used to gain unique insights into the regulation of cellular signal transduction. Genetically encodable fluorescent biosensors have allowed researchers to observe biochemical processes within the endogenous cellular environment with unprecedented spatiotemporal resolution. As the number and diversity of available biosensors grows, it is increasingly important to equip researchers with an understanding of the key concepts underlying the design and application of genetically encodable fluorescent...
Degenerative Spinal Deformity: Creating Lordosis in the Lumbar Spine, An Issue of Neurosurgery Clinics of North America E-Book
This issue of Neurosurgery Clinics, edited by Drs. Sigurd Berven and Praveen V. Mummaneni, will cover Degenerative Spinal Deformity: Creating Lordosis in the Lumbar Spine. Topics will include, but are not limited to, Spinopelvic Parameters; Location of lordosis (priority for L4-S1) and Age Adjustments; Approach Selection; Nuances of Pedicle Subtraction Osteotomy; Preventing Pseudarthrosis and PJK; The Challenge of Creating Lordosis in High Grade Dysplastic Spondylolisthesis; Sacropelvic Fixation; Evolution of the MISDEF Algorithm; Transpsoas Approach Nuances; Lateral Prepsoas Approach Nuances; Anterior Column Release; Navigation assisted MIS deformity correction; MIS TLIF; MIS PSO; and The challenge of L4-S1- fractional curves.
OPLL
This publication brings together information on all aspects of OPLL - epidemiology, etiology, diagnosis, and treatment. It contains contributions by Japanese researchers and surgeons, including members of the Ministry of Health and Welfare Investigation Committee, and by American surgeons with expertise in the field. Until now, little has been published on the subject in English. This collection of reports is amply augmented with illustrations.
Activating and Inhibitory Immunoglobulin-like Receptors
A remarkable spectrum of novel immunoreceptors sharing related immunoglobulin-like domains and signaling potential has been identified in recent years. These receptors have attracted widespread interest because they resemble the TCR, BCR, and FcR complexes in their ability to serve as activating or inhibitory receptors on the cells that bear them. Moreover, they are well positioned to affect both innate and adaptive immunity. The full range of ligands for these new receptor families is still not known, and understanding of their physiological roles is far from complete. This volume is the first attempt to summarize and highlight all known aspects of immunoglobulin-like receptors, providing a topical overview of the roles and characteristic features of the immunoglobulin-like receptors and related molecules in the immune system. Researchers in immunology, molecular biology, cell biology, clinical medicine, and pharmacology will find this book invaluable.
The Nature of the Mechanical Bond
“The story is told by THE inventor-pioneer-master in the field and is accompanied by amazing illustrations… [it] will become an absolute reference and a best seller in chemistry!” Alberto Credi “… the great opus on the mechanical bond. A most impressive undertaking!” Jean-Marie Lehn Congratulations to co-author J. Fraser Stoddart, a 2016 Nobel Laureate in Chemistry. In molecules, the mechanical bond is not shared between atoms—it is a bond that arises when molecular entities become entangled in space. Just as supermolecules are held together by supramolecular interactions, mechanomolecules, such as catenanes and rotaxanes, are maintained by mechanical bonds. This emergent bond ...
Yeast Surface Display
In addition to research and discovery, yeast surface display technology has found applications in industrial processes such as biofuel production and environmental pollutant absorption and degradation. Yeast Surface Display: Methods, Protocols, and Applications guides readers through yeast surface antibody display library and antibody engineering, yeast surface display as a tool for protein engineering, yeast surface cDNA display library construction and applications, and yeast surface display in bioassay and industrial applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Concise and easy-to-use, Yeast Surface Display: Methods, Protocols, and Applications aims to help accelerate the work of protein chemists, antibody engineers, molecular and cell biologists, and industrial bioengineers.
Stereoselective Biocatalysis
This book offers an explanation of the specific ways that biocatalysis outperforms chemical catalysis by: utilizing ambient temperature and atmospheric pressure to minimize problems of isomerization, racemization, and epimerization; employing microbial cells and enzymes that can be immobilized and reused over many cycles; and overexpressing enzymes for greater economy and efficiency.
Templates in Chemistry I
With contributions by numerous experts
