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Molecular Interactions in Bioseparations
Rapid advances in molecular biology have accelerated the production of a great number of protein-based therapeutic agents. The major cost in producing these proteins appears to be associated with their purification from the complex mixture of the crude extract. A major challenge to the protein biochemist and the biochemical engineer is the development of rapid, efficient, and cost-effective purification systems. This volume presents state-of-the-art reviews of current methods used in the purifica tion of biological macromolecules that are based on molecular interactions. Thus, the major emphasis is placed on affinity-related techniques. Part I provides a general introduction to affinity chro...
Annual Reports on Fermentation Processes
Annual Reports on Fermentation Processes, Volume 6 focuses on the significant developments in fermentation processes. This book discusses the application of immobilized living microbial cells, aerobic high-rate process for concentrated wastes, and large-scale cell culture technology. The gas-liquid mass transfer fermentation step, aeration devices, relative motion of cells and medium, and enzymes of industrial interest are also elaborated. This text likewise covers the microbial sensors for waste waters control, ventures in yeast utilization, and advances in D-xylose conversion by yeasts. Other topics include the effect of high energy radiation on lignocellulose, interaction between the solid substrate and the enzyme, and control and operation of fed-batch processes. This volume is valuable to students and researchers aiming to acquire knowledge of fermentation research and developments.
Immobilized Microbial Cells
Immobilized Microbial Cells, Volume 4 provides an overview of the methods of immobilization, applications, and ways of utilizing immobilized microbial cells and subcellular organelles and chloroplasts as biocatalysts. This volume is comprised of seven chapters. It begins with the historical background of immobilized cell research. Subsequent chapters focus on the methods of immobilization and applications of immobilized microbial cells, living cells, and organelles. The last two chapters discuss gas production of immobilized cells for energy generation and the chemical engineering analysis of immobilized-cell systems. The book will be of great use to chemists and chemical engineers.
Isolation and Purification of Proteins
This publication details the isolation of proteins from biological materials, techniques for solid-liquid separation, concentration, crystallization, chromatography, scale-up, process monitoring, product formulation, and regulatory and commercial considerations in protein production. The authors discuss the release of protein from a biological host, selectivity in affinity chromatography, precipitation of proteins (both non-specific and specific), extraction for rapid protein isolation, adsorption as an initial step for the capture of proteins, scale-up and commercial production of recombinant proteins, and process monitoring in downstream processing.
Insect Cell Culture Engineering
Consolidating and expanding current, fundamental notions of virology and animal cell cultivation, this practical reference examines the development of insect cell culture techniques for the production of recombinant proteins and insect pathogenic viruses.;Resolving on-the-job problems such as sparging cell damage and reduced infectivity cells, Insect Cell Culture Engineering: includes special introductory material as well as background information on insect pathogenic viruses, the molecular biology of baculoviruses and bioreactor design; offers advice on how to save time when deciding which insect cell line, bioreactor and medium to exploit; discusses the preparation of mathematical modellin...
Cell Adhesion in Bioprocessing and Biotechnology
Offers a detailed introduction to the fundamental phenomena that govern cell adhesion and describes bioengineering processes that employ cell adhesion, focusing on both biochemical and biomedical applications. All industrially relevant issues of cell adhesion - from basic concepts, quantitative experiments, and mathematical models to applications in bioreactors and other process equipment - are examined.
Chemistry for the Future
Chemistry for the Future covers the proceedings of the 29th IUPAC Congress on the Chemistry for the Future, held in Cologne, Federal Republic of Germany on June 5-10, 1983. The contributors consider the advances in inorganic, organic, physical, and theoretical chemistry. This book is organized into seven parts encompassing 59 chapters that also look into the progress in the production of chemical basic materials and education in chemistry. The opening parts survey the advances in complexation chemistry, photoelectrochemical energy conversion, biotechnology, and some aspects of inorganic chemistry. The succeeding part deals with the reactions, synthesis, and structure and properties determina...
PAT Applied in Biopharmaceutical Process Development And Manufacturing
As with all of pharmaceutical production, the regulatory environment for the production of therapeutics has been changing as a direct result of the US FDA-initiated Quality by Design (QbD) guidelines and corresponding activities of the International Committee for Harmonization (ICH). Given the rapid growth in the biopharmaceutical area and the comp
Immobilized Enzyme Technology
On November 5-8, 1974, a Seminar on Research and De velopment of Immobilized Enzymes was held in Tokyo, Japan. The seminar was part of the United States-Japan Cooperative Science Program sponsored jointly by the National Science Foundation and the Japan Society for the Promotion of Sci ence. The purpose of the seminar was to promote the scienti fic exchange of ideas and scien~ific results of a practical nature, as well as academic advances made in both countries through discussion and exchange of ideas. The areas chosen for discussion included: fundamental research in immobilized enzymes, new techniques of enzyme purification, comparative studies on immobilization techni ques and the relativ...
