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FRET and FLIM Techniques
This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields.
Microscopy Techniques
With contributions by numerous experts
Root Hairs
Root hairs are tip-growing cells that originate from epidennal cells called trichoblasts. Their role may be simply thought of as extending the surface area of the root to facilitate absorption of nutrients and water. However, as you will see in this book, the root hair is far more than that. To an increasingly larger number of plant biologists, the root hair is a model cell. It grows in much the same way as a pollen tube, by sending vast numbers of vesicles containing cell wall precursors to a rounded apical dome, the tip. Once the trichoblast becomes committed to root hair fonnation, it no longer divides. The root hair cell has a migrating nucleus and a complex cytoskeleton. It has a varied...
Biological Signal Transduction
Proceedings of the NATO Advanced Research Institute on Biological Signal Transduction held on the Island of Spetsai (Greece), August 6 - 17, 1990
Fluorescent and Luminescent Probes for Biological Activity
The use of fluorescent and luminescent probes to measure biological function has increased dramatically since publication of the First Edition due to their improved speed, safety, and power of analytical approach. This eagerly awaited Second Edition, also edited by Bill Mason, contains 19 new chapters and over two thirds new material, and is a must for all life scientists using optical probes.The contents include discussion of new optical methodologies for detection of proteins, DNA and other molecules, as well as probes for ions, receptors, cellular components, and gene expression. Emerging and advanced technologies for probe detection such as confocal laser scanning microscopy are also cov...
Cellular Fatty Acid-Binding Proteins II
Twenty years have elapsed since cytoplasmic proteins exhibiting high-affinity binding of long-chain fatty acids were first identified (Ockner et al., Science 177:56-58, 1972). These cellular fatty acid-binding proteins (FABPs) are now well established to comprise a ligand-defined group of macromolecules belonging to a family of cytoplasmic lipid binding proteins. Unique features of the FABPs are the existence of distinct types of FABP and that these are found in a variety of tissues in remarkable abundance, with some cells expressing more than one type. The physiological significance of the FABPs has only partly been elucidated. By increasing the cytoplasmic solubilization of fatty acids, th...
Root Hairs
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Imaging from Cells to Animals In Vivo
Imaging from Cells to Animals In Vivo offers an overview of optical imaging techniques developed over the past two decades to investigate biological processes in live cells and tissues. It comprehensively covers the main imaging approaches used as well as the application of those techniques to biological investigations in preclinical models. Among the areas covered are cell metabolism, receptor-ligand interactions, membrane trafficking, cell signaling, cell migration, cell adhesion, cytoskeleton and other processes using various molecular optical imaging techniques in living organisms, such as mice and zebrafish. Features Brings together biology and advanced optical imaging techniques to pro...
The Journal of Cell Biology
No. 2, pt. 2 of November issue each year from v. 19 (1963)-47 (1970) and v. 55 (1972)- contain the Abstracts of papers presented at the Annual Meeting of the American Society for Cell Biology, 3d (1963)-10th (1970) and 12th (1972)-
