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Is noninvasive, risk-free prenatal diagnosis of fetal genetic characteristics still a fantasy, or will it soon become reality? The current status of both the leading European research groups as well as the NIH-funded NIFTY study are reported here, indicating that certain fetal genetic traits can now be examined efficiently in a noninvasive manner. Considerable focus is placed on new laser-mediated systems for the effective micro-manipulation of single fetal cells, as well as their analysis by single-cell PCR and the pitfalls to avoid when performing such analyses. Other issues addressed in depth include: novel enrichment techniques, optimal fetal cell recognition, fetal cell culture, as well as the exciting finding that fetal cell traffic is elevated in certain pregnancy-related disorders, most prominently in preeclampsia. This publication is of interest to researchers in the field, genetic counsellors, gynecologists and obstetricians, and researchers in microchimerism, transplantation and transfusion medicine.
Following a section on tissue culture, chromosome staining and basic information about karyotyping, this text presents nomenclature and quality standards, as well as protocols of relevance to comprehensive cytogenetic diagnostics.
Cord blood is a significant source of hematopoietic stem and progenitor cells for the treatment of blood and genetic disorders. Cord blood is also an alternative to embryos as a source of tissue for regenerative medicine. Cells from cord blood have been shown to transdifferentiate into nonhematopoietic cells, including those of the brain, heart, liver, pancreas, bone, and cartilage, in tissue culture and in animal systems. Recently it has been demonstrated that both cardiac and glial cell differentiation of cord blood donor cells occurred in recipients of unrelated donor cord blood transplantation as part of a treatment regime for Krabbe disease and Sanfilippo syndrome. These observations raise the possibility that cord blood may serve as a source of cells to facilitate tissue repair and regeneration in the future. CD34 stem cell-rich umbilical cord whole blood transfusion has the potential to have an immediate benefit of better tissue oxygenation with an additional delayed benefit of possible engraftment of umbilical cord stem cells.
Obstetrics: Normal and Problem Pregnancies: First South Asia Edition remains your go-to choice for authoritative guidance on managing today's obstetric patient. International experts put the latest knowledge in this specialty at your fingertips, with current and relevant information on everything from fetal origins of adult disease, to improving global maternal health, to important topics in day-to-day obstetric practice. Highly readable, well-illustrated, and easy to understand, this best-selling obstetrics reference is an ideal tool for residents and clinicians. • Sweeping updates appear throughout, including four new chapters: "Vaginal Birth After Cesarean Delivery," "Placenta Accreta,"...
The Self, and Other Stories is an autoethnographic reflection on the value in the act of writing, illuminating the life of the researcher—in particular the researcher as human. Shepherd explores the multitudes of the academic, feminist self through expanding vocabularies of how scholars, researchers, writers, teachers, and academics can make sense of their worlds. At the intersection of international relations theory and the personal, Shepherd presents seven reflexive essays on aspects of being and knowing as she has encountered them. The essays are grounded in and inspired by her experiences as a way of asking readers to imagine how knowledge production in the social sciences might look different if we could create and hold space for different ways of writing, being, and knowing. The disciplining practices which produce our limited modes of academic expression can be encountered otherwise. She calls on us to reflect on academic subjectification across the interconnected spaces we simultaneously inhabit and produce.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences. - Basic Principles, Specialized Uses, and Genetic Applications - LCM and its application in genomics and proteomics - Fluorescence in situ hybridization of LCM isolated nuclei from paraffin sections - Noncontact laser catapulting for the functional genomics and proteomics - Use of LCM for clonal analysis, in carcinoma analysis, to assess development, in complex tissue, in pathology, gene discovery, and more
Highly readable, well illustrated, and easy to understand, Obstetrics: Normal and Problem Pregnancies remains your go-to choice for authoritative guidance on managing today's obstetric patient. Reflecting the expertise of internationally recognized authorities, this bestselling obstetrics reference has been thoroughly revised to bring you up to date on everything from ultrasound assessment of fetal anatomy and growth, to medical complications in pregnancy, to fetal therapy...and much more! 'An excellent reference for residents and fellows in training alike' Reviewed by: Obstetrics and Prenatal Diagnosis Unit, Landspitali University Hospital/University of Iceland Date: December 2014 Benefit f...
Fetal medicine has emerged as a separate subspecialty over the last 30 years as a result of major advances in a number of areas, in particular ultrasound imaging, cytogenetics, molecular biology and biochemistry. The widespread use of antenatal screening and diagnostic tests has led to an increased need for obstetricians to have knowledge and skills in fetal medicine. This book provides the information that underpins training programmes in fetal medicine and integrates science and clinical disciplines in a practical and useful way. Clinical sections include: the latest advances in prenatal screening; a systems-based presentation of the diagnosis and management of fetal malformations; complete coverage of common and rare fetal conditions including growth restriction, endocrine and platelet disorders, early pregnancy loss, and twins/multiple pregnancy. More focus on important basic-science concepts, such as maternofetal cell trafficking, and the relevance to clinical management.
Many diseases earlier considered to be incurable are now being treated with modern innovations involving fetal tissue transplants and stem cells derived from fetal tissues. Fetal tissues are the richest source of fetal stem cells as well as other varying states of differentiated cells and support or stromal cells. The activity of such stem cells is at their peak provided they are given the correct niche. Stem cells, as we know, are immortal cells with the capacity to regenerate into any kind of differentiated cell as per niche-guidance. As such, fetal tissues have the potential capacity to mend, regenerate and repair damaged cells or tissues in adults, when directly transplanted to the site ...
Doody Rating : 3 stars : The book "Donald School Manual of Practical Problems in Obstetrics" is a comprehensive review of problems that may occur during pregnancy and labour. Obstetrics is the fastest changing branch of medicine and needs extra care as the obstetrician looks after two lives during the antenatal period. The contents of the book cover all types of problems during pregnancy and labour and every aspect of obstetrics management. The layout of the book is simple and attracts, text is lucid for easy read and write. The book consists of thirty nine chapters, well arranged in a coherent manner. Several chapters are dedicated to labour and include topics such as instrumental delivery,...