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T-Cell Development
This second edition volume provides new and updated chapters detailing simple and accessible experiment protocols to explore thymus biology. Chapters are divided into three parts presenting short reviews, analysis strategies, protocols for cell preparation, flow cytometry analyses, Innate Lymphoid Cells (ILC), mouse T-cell development, antigen receptor-less cousins of T cells, bone marrow chimeras, thymic stroma, and multiple aspects of thymocyte biology. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, T-Cell Development: Methods and Protocols, Second Edition aims to be a useful practical guide to help readers overcome obstacles associated with experimental approaches of T-cell development.
Cultivation Chat Group 3 Anthology
One day, Song Shuhang was suddenly added to a chat group with many seniors that suffered from chuuni disease. The people inside the group would call each other ‘Fellow Daoist’ and had all different kinds of titles: Palace Master, Cave Lord, True Monarch, Immortal Master, etc. Even the pet of the founder of the group that had run away from home was called ‘monster dog’. They would talk all day about pill refining, exploring ancient ruins, or share their experience on techniques. However, after lurking inside the group for a while, he discovered that not all was what it seemed...
Cultivation Chat Group 6 Anthology
One day, Song Shuhang was suddenly added to a chat group with many seniors that suffered from chuuni disease. The people inside the group would call each other ¡®Fellow Daoist¡¯ and had all different kinds of titles: Palace Master, Cave Lord, True Monarch, Immortal Master, etc. Even the pet of the founder of the group that had run away from home was called ¡®monster dog¡¯. They would talk all day about pill refining, exploring ancient ruins, or share their experience on techniques. However, after lurking inside the group for a while, he discovered that not all was what it seemed...
Cultivation Chat Group 5 Anthology
One day, Song Shuhang was suddenly added to a chat group with many seniors that suffered from chuuni disease. The people inside the group would call each other ¡®Fellow Daoist¡¯ and had all different kinds of titles: Palace Master, Cave Lord, True Monarch, Immortal Master, etc. Even the pet of the founder of the group that had run away from home was called ¡®monster dog¡¯. They would talk all day about pill refining, exploring ancient ruins, or share their experience on techniques. However, after lurking inside the group for a while, he discovered that not all was what it seemed...
Cultivation Chat Group 2 Anthology
One day, Song Shuhang was suddenly added to a chat group with many seniors that suffered from chuuni disease. The people inside the group would call each other ‘Fellow Daoist’ and had all different kinds of titles: Palace Master, Cave Lord, True Monarch, Immortal Master, etc. Even the pet of the founder of the group that had run away from home was called ‘monster dog’. They would talk all day about pill refining, exploring ancient ruins, or share their experience on techniques. However, after lurking inside the group for a while, he discovered that not all was what it seemed...
Single Cell Sequencing and Systems Immunology
The volume focuses on the genomics, proteomics, metabolomics, and bioinformatics of a single cell, especially lymphocytes and on understanding the molecular mechanisms of systems immunology. Based on the author’s personal experience, it provides revealing insights into the potential applications, significance, workflow, comparison, future perspectives and challenges of single-cell sequencing for identifying and developing disease-specific biomarkers in order to understand the biological function, activation and dysfunction of single cells and lymphocytes and to explore their functional roles and responses to therapies. It also provides detailed information on individual subgroups of lympho...
Cultivation Chat Group 1 Anthology
One day, Song Shuhang was suddenly added to a chat group with many seniors that suffered from chuuni disease. The people inside the group would call each other ¡®Fellow Daoist¡¯ and had all different kinds of titles: Palace Master, Cave Lord, True Monarch, Immortal Master, etc. Even the pet of the founder of the group that had run away from home was called ¡®monster dog¡¯. They would talk all day about pill refining, exploring ancient ruins, or share their experience on techniques. However, after lurking inside the group for a while, he discovered that not all was what it seemed...
