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Methods In Arabidopsis Research
One of the ways to make consistent progress in a particular field of biology consists in choosing a good model system on which to focus the experimental efforts of the scientific community. It has taken a long time for scientists interested in various aspects of the life of plants to reach some sort of consensus. With the advent and impact of molecular biology, the small weed Arabidopsis is now the object of rapidly growing scientific attention. Since it is reasonable to assume that the general molecular mechanisms that are responsible for the physiological, cellular and biochemical properties of plants will be essentially conserved in all plants, it follows that these mechanisms should also...
Arabidopsis Thaliana
Plants are sessile organisms and their only alternative to a rapidly changing environment is a fast adaptation to abiotic and biotic stresses. Among the several known species of flowering plants, Arabidopsis thaliana is the only plant that has been most thoroughly studied. This angiosperm with dicotyledonous seeds belonging to the family Brassicaceae was known to botanists for at least four centuries and has been used since then for experimental studies for about half a century, until it was Friedrich Laibach who had outlined the advantages of using it in genetic experiments and had also suggested that it could be used as a plant model system in 1943. Its unique features favors genetic exper...
Arabidopsis
Focuses on Arabidopsis, one of the important model systems available for gaining an understanding of gene organization, regulation, and development in flowering plants at the molecular level. This work examines global elements of the Arabidopsis genome project, the construction of the physical map and strategies for structure function analysis.
Arabidopsis Thaliana as a Model for Plant-pathogen Interactions
Presents techniques for examining this small weed's unique systems.
Arabidopsis
The recent application of molecular genetics to problems of developmental biology has provided us with greater insight into the molecular mechanisms by which cells determine their developmental fate. This is particularly evident in the recent progress in understanding of developmental processes in model animal systems such as Drosophila melanogaster and Caenorhabditis elegans. De spite the use of plants in some of the earliest genetics experiments, the elucida tion of the molecular bases of plant development has lagged behind that of animal development. However, the emergence of model systems such as Arabi dopsis thaliana, amenable to developmental genetics, has led to the beginning of the u...
Arabidopsis
The thale cress Arabidopsis thaliana is increasingly popular among plant scientists: it is small, easy to grow, and makes flowers, and the sequence of its small and simple genome was recently completed. This is the most complete and authoritative laboratory manual to be published on this model organism and the first to deal with genomic and proteomic approaches to its biology.
Arabidopsis
Arabidopsis has long been acknowledged as the 'Botanical Drosophila' with its small genome, low levels of repetitive DNA, small size and fast generation time it is an ideal molecular genetic tool for the analysis of development in higher plants. Arabidopsis: A Practical Approach provides an introduction to most of the key techniques required for the use of Arabidopsis as an experimental system. It gives a basic introduction to the optimal growth conditions and genetic resources available for Arabidopsis, how this material should be handled, maintained and used. Individual chapters describe strategies for the identification, mapping (using multi-marker lines and recombinant inbreds), and characterisation of different mutants by microscopy, molecular cytogenetics and gene expression analysis. Different cloning strategies, using transposons, T-DNA and map position are described in detail. Sequencing of the Arabidopsis genome will be completed in 2000 and bioinformatics are of key importance; the tools that are available and where they can be found on the Web are presented.
