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Plant Gene Regulatory Networks
This volume presents protocols that analyze and explore gene regulatory networks (GRNs) at different levels in plants. This book is divided into two parts: Part I introduces different experimental techniques used to study genes and their regulatory interactions in plants. Part II highlights different computational approaches used for the integration of experimental data and bioinformatics-based predictions of regulatory interactions. This part of the book also provides information on essential database resources that grant access to gene-regulatory and molecular interactions in different plant genomes, with a specific focus on Arabidopsis thaliana. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Plant Gene Regulatory Networks: Methods and Protocols is a valuable resource for scientists and researchers interested in expanding their knowledge of GRNs.
Plant Membrane and Vacuolar Transporters
This book contains 14 chapters that evaluate the current knowledge, state of art and future prospects of plant membrane and vacuolar transporters in relation to plant productivity and quality improvement. The role of transporters, the mechanisms of uptake, accumulation or transport of potassium, calcium, nitrate, ammonium, sulfate, phosphate, iron, manganese, silicon, heavy metals, sugars, polyols, amino acids and secondary metabolites, the proteomic analysis of vacuolar membrane, and the elemental biofortification of crop plants are emphasized. The book is intended for scientists, researchers, students, planners and industrialists working in the area of biotechnology, plant agriculture, agronomy, horticulture, plant physiology, molecular biology, nutritional biology, plant sciences and environmental sciences.
Functional Characterization of Arabidopsis Phosphatidylinositol Monophosphate 5-kinase 2 in Lateral Root Development, Gravitropism and Salt Tolerance
The functional characterization of a key enzyme in the phosphatidylinositol (PI) signaling pathway in the model plant Arabidopsis thaliana is the focus of the research summarised in this thesis. Moreover, a particular focus is the exploration of the biological functions of Arabidopsis phophatidylinositol monophosphate 5-kinase 2 (PIP5K2) which catalyzes the synthesis of phophatidylinositol (4,5) bisphosphate, the precursor of two important second messengers (inositol 1,4,5-trisphosphate and diacylglycerol). Through molecular and genetic approaches, the author isolated and characterized the expression pattern, physiological functions and the underlying mechanism of Arabidopsis PIP5K2. It is found that PIP5K2 is involved in regulating lateral root formation and root gravity response through modulating auxin accumulation and polar auxin transport and also plays a critical role in salt tolerance. These findings shed new light on the crosstalk between PI signaling and auxin response, both of which have crucial regulatory roles in plant development.
Molecular Physiology and Ecophysiology of Sulfur
This proceedings volume contains a selection of invited and contributed papers of the 9th International Workshop on Sulfur Metabolism in Plants, which was hosted by Heinz Rennenberg, Albert-Ludwigs-University Freiburg and was held at Schloss Reinach, Freiburg-Munzigen, Germany from April 14-17, 2014. The focus of this workshop was on molecular physiology and ecophysiology of sulfur in plants and the content of this volume presents an overview on the current research developments in this field.
Ecophysiology of Tropical Plants
Plants in tropical regions are coping with enormous challenges of physiological stresses owing to changing environmental and climatic conditions. Rapid growth of human population and rampant exploitation of fossil fuels and other developmental activities are actively contributing to such perturbations. The Intergovernmental Panel on Climate Change has projected a sustained increase in carbon dioxide (CO2) emissions and thereby a rise in global temperature in the coming decades. The resultant changes in precipitation patterns are now evident across the globe due to intensication of hydrological cycle. Moreover, gaseous and particulate pollutants are also an immense challenge for tropical plan...
High Confidence Network Predictions from Big Biological Data
Biology functions in a most intriguing fashion, with human cells being regulated by multiplex networks of proteins and their dependent systems that control everything from proliferation to cell death. Notably, there are cases when these networks fail to function properly. In some diseases there are multiple small perturbations that push the otherwise healthy cells into a state of malfunction. These maladies are referred to as complex diseases, and include common disorders such as allergy, diabetes type II, and multiple sclerosis, and due to their complexity there is no universally defined approach to fully understand their pathogenesis or pathophysiology. While these perturbations can be mea...
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Delivery and Perception of Pathogen Signals in Plants
An overview of the past 50 years of plant microbe research heads 26 papers from the 8th Japan-US Cooperative Science Seminar on "Plant perception of pathogen signals" held in June 1999 in Marina del Ray, CA. Parallels between plant and animal host defense systems is a major theme. Historical topics of interest that continue to draw attention include: growth hormones, fungal and bacterial toxins, the avirulent gene concept, pathogen elicitors and hypersensitive responses, and the avirulence and harpin molecules. A new seminar focus is Hrp delivery system and apoptosis. Keen is at the U. of California, Riverside. Lacks an index. Annotation copyrighted by Book News, Inc., Portland, OR.
Plant Cold Acclimation
Plant Cold Acclimation: Methods and Protocols details many of the methods and protocols commonly used to study plant cold acclimation and freezing tolerance, breeding, genetics, physiology or molecular biology, or any combination of these specialties. Chapters focus on interdisciplinary approaches, experimental methods, and concepts from different areas of science. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Cold Acclimation: Methods and Protocols seeks to help not only new researchers starting in this field, but also those already working in a particular area of cold acclimation and freezing tolerance research who are looking to expand their range of experimental approaches.
Life at the Speed of Light
In 2010, scientists led by J. Craig Venter became the first to successfully create 'synthetic life' -- putting humankind at the threshold of the most important and exciting phase of biological research, one that will enable us to actually write the genetic code for designing new species to help us adapt and evolve for long-term survival. The science of synthetic genomics will have a profound impact on human existence, including chemical and energy generation, health, clean water and food production, environmental control, and possibly even our evolution. In Life at the Speed of Light, Venter presents a fascinating and authoritative study of this emerging field from the inside -- detailing its origins, current challenges and controversies, and projected effects on our lives. This scientific frontier provides an opportunity to ponder anew the age-old question 'What is life?' and examine what we really mean by 'playing God'. Life at the Speed of Light is a landmark work, written by a visionary at the dawn of a new era of biological engineering.
