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A collection of cutting-edge laboratory techniques for the study of trophoblast and placental biology. The techniques presented range from experimental animal models, to animal and human placental organ and cell culture systems, to morphological, biochemical, and molecular strategies for assessing trophoblast/placental growth, differentiation and function. Volume 1 provides readily reproducible protocols for studying embryo-uterine implantation, trophoblast cell development, and the organization and molecular characterization of the placenta. Highlights include strategies for the isolation and culture of trophoblast cells from primates, ruminants, and rodents, and precise guidance to the molecular and cellular analysis of the placental phenotype. A companion second volume concentrates on methods for investigating placental function.
Covert Operations... Edwin Amis, new commander of the Eridani Light Horse, faces a tough decision: Should he openly defy Sun-Tzu Liao, the First Lord of the Star League? The First Lord has sent Star League troops as "peacekeepers" to the St. Ives Compact to prevent open rebellion by angry citizens—but they're not seeking peace. Amis knows the mission is only a guise for reclaiming territory for the Capellan Confederation, and he's ready to station counter-forces on Milos, close to the heart of the Capellan capital. But on Milos, things are already escalating—Death Commando Chen Shao and his ruthless partner, Nessa Ament, have secretly murdered a dissident's family in cold blood. Now both forces are at each other's throats in what could erupt into full-scale war unless Amis can find a way to defuse the situation before more blood is shed...on a planetary scale...
All new Sherlock Holmes adventures as taken from audio scripts of radio shows that have been syndicated by Imagination Theater. In these all new adventures of the great consulting detective, writers Steven Philip Jones and Matthew J. Elliot manage to honor the world of Sir Arthur Conan Doyle while creating rich and exciting tales. Each tale is accompanied by an original illustration by Joshua Werner, and an introduction from Larry Albert, the voice of Dr. Watson on the radio series. Contained within are FOUR tales: THE ADVENTURE OF THE BORGIA RING - Judith Oxley wants to hire Sherlock Holmes to find her missing father, Lord Oxley, along with their family’s prize possession, the Akbar Shah ...
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AMPK has emerged as an important integrator of signals that control energy balance through the regulation of multiple biochemical pathways in eukaryotes. This book focuses on the implications of AMPK as a master metabolic regulator in diseases, including new methods and animal models. The contributions are written by leading experts in the field and give an extensive overview of the current knowledge of AMPK biology and the role of AMPK in health and disease.
"I always thought the job was to be as great as you could be." - Bono This is the story of U2, in their own words and pictures.