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Alan V. Smrcka presents a collection of cutting-edge methods for investigating G protein signaling from a variety of perspectives ranging from in vitro biochemistry to whole animal studies. Among the readily reproducible techniques presented are those for the purification of G proteins and effectors enzymes, assays of these purified G proteins and effector enzymes, and for the study of G proteins interactions with effectors in intact cells. Additional methods are provided for assaying G protein coupled receptor structure, function, and localization, and for studying the physiological roles for endogenous G proteins.
Finally! There is a definitive reference guide available for harmonicas in each and every key. This ground breaking series unlocks the musical power of the 10-hole major diatonic harmonica. One key at a time, each is designed to present detailed musical information for beginners, intermediate and advanced players who are either music readers or non-reading players. Learn the notes, intervals, bends, overbends, dyads, chords, arpeggios, modes and scales that are specific to each harmonica key. There is also a special section in each book showing other types of diatonic harmonicas and the variations unique to them like—extreme bending, low-tuned, octave and tremolo-tuned models and more.Have...
Slack enjoyed full access to Hutchinson's archives and conducted extensive interviews both with Hutchinson himself and with his students, colleagues, and friends. She evaluates his contributions to theoretical ecology, limnology (the study of fresh-water ecosystems), biogeochemistry, population ecology, and the creation of the new fields of systems ecology and radiation ecology, and she discusses his profound influence as a mentor. The book also looks into his personal life, which included three very different wives, a refugee baby under his care during World War II, friendships with such contemporaries as Rebecca West, Margaret Mead, and Gregory Bateson, and a host of colleagues and friends on four continents. Filled with information available nowhere else, this book draws a vibrant portrait of a giant in the discipline of twentieth-century ecology who was also a man of remarkable personal appeal. --Book Jacket.
This volume covers topics such as the structure and identification of functional domains of G proteins, and activation of G proteins by receptors or other regulators. The text takes an integrated approach to studying common experimental questions at many different levels related to G proteins. Methods related to G proteins using molecular modeling, systems biology, protein engineering, protein biochemistry, cell biology, and physiology are all accessible in the same volume. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant todaytruly an essential publication for researchers in all fields of life sciences.
The past decade has seen tremendous advances in the study of G protein-coupled receptors (GPCRs), including the molecular cloning and identification of more than 100 hundred GPCR genes. But while GPCRs serve as targets for more than 300 medicines in the modern pharmacopoeia, the shrinking pool of known ligands and the continuing discovery of orphan GPCR genes have underscored the need for new approaches to ligand identification. Identification and Expression of G Protein-Coupled Receptors addresses this new direction in GPCR biochemistry-offering a definitive laboratory bench manual that emphasizes expression over primary cloning strategies. In a series of expert contributions by well-known ...
G-protein-coupled receptors (GPCRs) are believed to be the largest family of membrane proteins involved in signal transduction and cellular responses. They dimerize (form a pair of macromolecules) with a wide variety of other receptors. The proposed book will provide a comprehensive overview of GPCR dimers, starting with a historical perspective and including, basic information about the different dimers, how they synthesize, their signaling properties, and the many diverse physiological processes in which they are involved. In addition to presenting information about healthy GPCR dimer activity, the book will also include a section on their pathology and therapeutic potentials.