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Advances in Clinical Chemistry
Advances in Clinical Chemistry, Volume 75, the latest installment in this internationally acclaimed series, contains chapters authored by world-renowned clinical laboratory scientists, physicians, and research scientists. This serial discusses the latest and most up-to-date technologies related to the field of clinical chemistry and is the benchmark for novel analytical approaches in the clinical laboratory. - Contains the expertise of international contributors - Provides the latest cutting-edge technologies in the field of clinical chemistry - Authored by world-renowned clinical laboratory scientists, physicians, and research scientists
2-D Proteome Analysis Protocols
With the completion of sequencing projects and the advancement of a- lytical tools for protein identification, proteomics—the study of the expressed part of the genome—has become a major region of the burgeoning field of functional genomics. High-resolution 2-D gels can reveal virtually all p- teins present in a cell or tissue at any given time, including posttranslationally modified proteins. Changes in the expression and structure of most cellular proteins caused by differentiation or external stimuli can be displayed and eventually identified using 2-D protein gels. 2-D Proteome Analysis Protocols covers all aspects of the use of 2-D protein electrophoresis for the analysis of biologi...
The Proteomics Protocols Handbook
Hands-on researchers describe in step-by-step detail 73 proven laboratory methods and bioinformatics tools essential for analysis of the proteome. These cutting-edge techniques address such important tasks as sample preparation, 2D-PAGE, gel staining, mass spectrometry, and post-translational modification. There are also readily reproducible methods for protein expression profiling, identifying protein-protein interactions, and protein chip technology, as well as a range of newly developed methodologies for determining the structure and function of a protein. The bioinformatics tools include those for analyzing 2D-GEL patterns, protein modeling, and protein identification. All laboratory-based protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Advances in Clinical Chemistry
Volume 47 in the internationally acclaimed Advances in Clinical Chemistry contains chapters submitted from leading experts from academia and clinical laboratory science. Authors are from a diverse field of clinical chemistry disciplines and diagnostics, ranging from basic biochemical exploration to cutting-edge microarray technology. - Leading experts from academia and clinical laboratory science - Volume emphasizes novel laboratory advances with application to clinical laboratory diagnostics and practical basic science studies
Smolin and Thoft's The Cornea
Smolin and Thoft’s The Cornea is often praised as the best available source of information on corneal and external diseases. This new edition, with its greatly expanded color atlas section, continues to provide guidance on diagnosing and managing problems associated with the cornea. It is now fully updated and contains additional information on corneal surgery, refractive surgery, and stem cell grafting, and a new chapter on optical and therapeutic contact lenses.
Proteins and Proteomics
Introduction to proteomics; one-dimensional polyacrylamide gel electrophoresis; preparing cellular and subcellular extracts; preparative two-dimensional gel electrophoresis with immobilized pH gradients; reversed-phase high -performance liquid chromatography; amino- and carboxy-terminal sequence analysis; peptide mapping and sequence analysis of gel-resolved proteins; the use of mass spectrometry in proteomics; proteomic methods for phosphorylation site mapping; characterization of protein complexes; making sense of proteomics - using bioinformatics to discover a protein's structure, functions, and interactions.
Phage Display In Biotechnology and Drug Discovery
The first and only guide to showcase the impact of phage display technology on drug discovery, this reference details the theories, principles, and methods impacting the field and demonstrates applications for peptide phage display, protein phage display, and the development of novel antibodies. Highlighting the current and future role of phage dis
Emotional Monasticism
Drawing on the devotional culture of John of Fécamp's Norman monastery, Emotional monasticism exposes the monastic roots of medieval affective piety, casts a new light on the devotional life of monks in Europe before the twelfth century and redefines how medievalists should teach the history of Christian devotion.
Biochemistry and Molecular Biology of Plants
Membrane structures are spatial structures made out of tensioned membranes. The structural use of membranes can be divided into pneumatic structures, tensile membrane structures, and cable domes. In these three kinds of structure, membranes work together with cables, columns and other construction members to find a form. Peripheral membrane proteins are found on the outside and inside surfaces of membranes, attached either to integral proteins or to phospholipids. Unlike integral membrane proteins, peripheral membrane proteins do not stick into the hydrophobic core of the membrane, and they tend to be more loosely attached. Cells are the smallest units of life. They are a closed system, can ...
Vitamins and Minerals in the Prevention and Treatment of Cancer
This book provides researchers and practitioners with a unique collection of current research on the role of vitamins and micronutrients in cancer prevention and treatment. New theories are discussed, including a hypothesis that dietary factors may protect against genetically predisposed cancers. Mechanisms by which different vitamins and minerals appear to inhibit carcinogenesis or cell transformation are described, including vitamins A, C, E, and selenium protection against oxidative stress by induction of enzymes as catalase and dismutase or inteference with free radical mechanisms; organosulfur compound inhibition of P450 activation enzymes or enhancement of detoxification enzymes; metal...
