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This book has been written to meet the needs of students for biotechnology courses at various levels of undergraduate and graduate studies. This book covers all the important aspects of plant tissue culture viz. nutrition media, micropropagation, organ culture, cell suspension culture, haploid culture, protoplast isolation and fusion, secondary metabolite production, somaclonal variation and cryopreservation. For good understanding of recombinant DNA technology, chapters on genetic material, organization of DNA in the genome and basic techniques involved in recombinant DNA technology have been added. Different aspects on rDNA technology covered gene cloning, isolation of plant genes, transpo...
Basics; Laboratory organization; Sterilization techniques; Nutrition medium; Choice of the explant; Plant tissue culture; Seed culture; Micropropagation- meristem culture; Micropropagation- axillary bud proliferation; Micropropagation- adventitious regeneration; Micropropagation- organogenesis; Micropropagation- embryogenesis; Cell suspension; Secondary metabolite production in a cell suspension culture; Anther culture; Protoplast isolation and fusion; Biotechnology; SDS-PAGE electrophoresis of proteins; Isolation of DNA from plant tissues; Isolation an purification of plasmid DNA; Restriction enzyme digestion of DNA; Agarose gel electrophoresis; Preparation of competent cells, transformation of E. coil with plasmid DNA and ligation of insert DNA to a vector; Agrobacterium-mediated gene transfer; Biolistic method of transformation in plants; In vitro amplification of DNA by PCR: detection of transgenes; RAPD analysis; Microsatellite marker analysis; Southerm biotting; Southerm hybridization.
Biochemistry provides a platform for convergence of all scientific knowledge about the operation of life and, therefore, it finds an important place in the curriculum of all the medical sciences. The present book is an attempt in this direction in the form of a student-friendly, yet comprehensive and up-to-date text.
Understanding and appreciating the intellectual property rights (IPRs), its nature and rationale for protection is a prerequisite in any degree programme. To meet course curriculum spheres was conceived with the assumption that the reader has little or no prior knowledge in IRPs. In each chapter a section on treaties, conventions or agreement. To meet the course curriculum requirement on the IPRs, a textbook a provide basic knowledge on the subject covering all the spheres was conceived with the assumption that the reader has little or no prior knowledge in IPRs.
What is a learning organization? What are the advantages of creating one? Why should a company want to become a learning organization? Where does one start? Learning Organizations: Developing Cultures for Tomorrow's Workplace contains essays by thirty-nine of the most respected practitioners and scholars of this topic. This definitive collection of essays is rich in concept and theory as well as application and example. Lead authors include Harvard's Rosabeth Moss Kanter, London Business School's Professor Emeritus Charles Handy, and MlT's Fred Kofman and Peter Senge. The thirty-two essays in this comprehensive collection are presented in four main parts: 1. Guiding Ideas 2. Theories/Methods/Processes 3. Infrastructure 4. Arenas of Practice
This book has been written to meet the needs of students for biotechnology courses at various levels of undergraduate and graduate studies. This book covers all the important aspects of plant tissue culture viz. nutrition media, micropropagation, organ culture, cell suspension culture, haploid culture, protoplast isolation and fusion, secondary metabolite production, somaclonal variation and cryopreservation. For good understanding of recombinant DNA technology, chapters on genetic material, organization of DNA in the genome and basic techniques involved in recombinant DNA technology have been added. Different aspects on rDNA technology covered gene cloning, isolation of plant genes, transpo...
Plant biotechnology has created unprecedented opportunities for the manipulation of biological systems of plants. To understand biotechnology, it is essential to know the basic aspects of genes and their organization in the genome of plant cells. This text on the subject is aimed at students.
The first book of its kind to review the current status and future direction of the exciting new branch of machine learning/data mining called imbalanced learning Imbalanced learning focuses on how an intelligent system can learn when it is provided with imbalanced data. Solving imbalanced learning problems is critical in numerous data-intensive networked systems, including surveillance, security, Internet, finance, biomedical, defense, and more. Due to the inherent complex characteristics of imbalanced data sets, learning from such data requires new understandings, principles, algorithms, and tools to transform vast amounts of raw data efficiently into information and knowledge representati...
This authoritative book--now revised and expanded with important clinical and research advances--presents a proven approach for helping people meet the day-to-day challenges of recovery from addiction and maximize their well-being. Mindfulness-based relapse prevention (MBRP) integrates carefully tailored meditation practices with cognitive and behavioral skills building. In a convenient large-size format, the book includes instructions for setting up and running MBRP groups, session-by-session implementation guidelines, sample scripts, and 27 reproducible handouts and forms. Purchasers can download and print the reproducible materials at the companion website, which also features audio recor...
Building on the strength of the previous two editions, Bergman's Comprehensive Encyclopedia of Human Anatomic Variation is the third installment of the classic human anatomical reference launched by Dr. Ronald Bergman. With both new and updated entries, and now illustrated in full color, the encyclopedia provides an even more comprehensive reference on human variation for anatomists, anthropologists, physicians, surgeons, medical personnel, and all students of anatomy. Developed by a team of editors with extensive records publishing on both human variation and normal human anatomy, Bergman's Comprehensive Encyclopedia of Human Anatomic Variation is the long awaited update to this classic reference.