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Gene Delivery to Mammalian Cells
The efficiency of delivering DNA into mammalian cells has increased t- mendously since DEAE dextran was first shown to be capable of enhancing transfer of RNA into mammalian cells in culture. Not only have other chemical methods been developed and refined, but also very efficient physical and viral delivery methods have been established. The technique of introducing DNA into cells has developed from transfecting tissue culture cells to delivering DNA to specific cell types and organs in vivo. Moreover, two important areas of biology—assessment of gene function and gene therapy—require succe- ful DNA delivery to cells, driving the practical need to increase the efficiency and efficacy of ...
Women in Science: Aging and Public Health 2022
The demographics of aging –whether differences in life expectancy or shouldering the burden of care for our aging populations—reflect that aging is indeed a women’s issue. In the field of Aging and Public Health, there are many highly influential and successful women who are contributing to the field and tackling important questions about risk factors for successful aging as well intervention strategies for promoting health and quality of life across the life-course.
Are We Making a Difference?
As the world experiences heightened levels of violent conflict and polarization, understanding what peacebuilding efforts are “effective” becomes all the more pressing. This groundbreaking edited volume brings together a diverse, global group of practitioners, researchers, and peacebuilders to grapple with urgent questions and challenges related to defining and assessing peacebuilding effectiveness. Sections of the book engage in critical reflection on what peacebuilding effectiveness is and who gets to decide, provide practical examples and case studies of the successes and failures of assessing peacebuilding work, and support innovative strategies and tools to move the field forward. C...
Bride
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Bio-nanoimaging
Bio-Nanoimaging: Protein Misfolding & Aggregation provides a unique introduction to both novel and established nanoimaging techniques for visualization and characterization of misfolded and aggregated protein species. The book is divided into three sections covering: - Nanotechnology and nanoimaging technology, including cryoelectron microscopy of beta(2)-microglobulin, studying amyloidogensis by FRET; and scanning tunneling microscopy of protein deposits - Polymorphisms of protein misfolded and aggregated species, including fibrillar polymorphism, amyloid-like protofibrils, and insulin oligomers - Polymorphisms of misfolding and aggregation processes, including multiple pathways of lysozyme...
The Natural World in Latin American Literatures
From the Popol Vuh to postmodernism, imagery of the natural world has played an important role in Latin American literature. In contrast to the rise of ecocritical scholarship in Anglophone literary studies, Latin American literary ecocriticism has been slower to take root. This volume of eleven essays seeks to advance the ecocritical conversation among Latin Americanists, furthering insight into the relationship between humans and their environments. The essays address regions as diverse as Patagonia and the Chihuahua Desert.
Diplomatic List
Directory of foreign diplomatic officers in Washington.
Advances in Chromatography
This is the 41st volume in the series Advances in Chromatography. The papers in this volume: take advantage of the properties of electroosmotic flow to explore capillary electrochromatography as an alternative to HPLC for nonvolatile analytes; analyze advances in biomembrane chromatography using reverse-phase HPLC for protein purification and biophysical studies of peptides and proteins at lipid-like surfaces; focus on trace metal determination and speciation performed by liquid chromatography methods; and more.
Cell Cycle Checkpoint Control Protocols
The field of cell cycle regulation is based on the observation that the life cycle of a cell progresses through several distinct phases, G1, M, S, and G2, occurring in a well-defined temporal order. Details of the mechanisms involved are rapidly emerging and appear extraordinarily complex. Furthermore, not only is the order of the phases important, but in normal eukaryotic cells one phase will not begin unless the prior phase is completed successfully. Che- point control mechanisms are essentially surveillance systems that monitor the events in each phase, and assure that the cell does not progress prematurely to the next phase. If conditions are such that the cell is not ready to progress...
