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Introduction to Optical Microscopy
This fully updated, self-contained textbook covering modern optical microscopy equips students with a solid understanding of the theory underlying a range of advanced techniques. Two new chapters cover pump-probe techniques, and imaging in scattering media, and additional material throughout covers light-sheet microscopy, image scanning microscopy, and much more. An array of practical techniques are discussed, from classical phase contrast and confocal microscopy, to holographic, structured illumination, multi-photon, and coherent Raman microscopy, and optical coherence tomography. Fundamental topics are also covered, including Fourier optics, partial coherence, 3D imaging theory, statistical optics, and the physics of scattering and fluorescence. With a wealth of end-of-chapter problems, and a solutions manual for instructors available online, this is an invaluable book for electrical engineering, biomedical engineering, and physics students taking graduate courses on optical microscopy, as well as advanced undergraduates, professionals, and researchers looking for an accessible introduction to the field.
Fundamentals of Light Microscopy and Electronic Imaging
Fundamentals of Light Microscopy and Electronic Imaging, Second Edition provides a coherent introduction to the principles and applications of the integrated optical microscope system, covering both theoretical and practical considerations. It expands and updates discussions of multi-spectral imaging, intensified digital cameras, signal colocalization, and uses of objectives, and offers guidance in the selection of microscopes and electronic cameras, as well as appropriate auxiliary optical systems and fluorescent tags. The book is divided into three sections covering optical principles in diffraction and image formation, basic modes of light microscopy, and components of modern electronic imaging systems and image processing operations. Each chapter introduces relevant theory, followed by descriptions of instrument alignment and image interpretation. This revision includes new chapters on live cell imaging, measurement of protein dynamics, deconvolution microscopy, and interference microscopy. PowerPoint slides of the figures as well as other supplementary materials for instructors are available at a companion website: www.wiley.com/go/murphy/lightmicroscopy
Handbook of Biomedical Nonlinear Optical Microscopy
The Handbook of Biomedical Nonlinear Optical Microscopy provides comprehensive treatment of the theories, techniques, and biomedical applications of nonlinear optics and microscopy for cell biologists, life scientists, biomedical engineers, and clinicians. The chapters are separated into basic and advanced sections, and provide both textual and graphical illustrations of all key concepts. The more basic sections are aimed at life scientists without advanced training in physics and mathematics, and tutorials are provided for the more challenging sections. The first part of the Handbook introduces the historical context of nonlinear microscopy. The second part presents the nonlinear optical th...
Quantum Measurements in Optics
The NATO Advanced Research Workshop on Quantum Measurements in Optics was held in Cortina d'Ampezzo, Italy, January 21-25, 1991. This workshop was attended by 70 participants from 16 different countries. The subjects discussed at this workshop concentrated on quantum measurements in optics made possible by the recent advances in the generation and detection of light with low quantum noise. These advances have occurred simultaneously with the development of atomic traps capable of trapping a single atom for a considerable period of time. The interaction of a single two level atom with the single mode of the electromagnetic field is now possible in high Q microcavities. A new field of cavity Q...
Physics of Complex Colloids
Colloids are systems comprised of particles of mesoscopic size suspended in a liquid. They have recently been attracting increased attention from scientists and engineers due to the fact that they are nowadays present in many industrial products such as paints, oil additives, electronic ink displays and drugs. Colloids also serve as versatile model systems for phenomena and structures from solid-state physics, surface science and statistical mechanics, and can easily be studied using tabletop experiments to provide insight into processes not readily accessible in atomic systems. This book presents the lectures delivered at the 2012 Enrico Fermi School ‘Physics of Complex Colloids’, held in Varenna, Italy, in July 2012. The school addressed experimental, theoretical and numerical results and methods, and the lectures covered a broad spectrum of topics from the starting point of the synthesis of colloids and their use in commercial products. The lectures review the state-of-the-art of colloidal science in a pedagogical way, discussing both the basics and the latest results, and this book will serve as a reference for both students and experts in this rapidly growing field.
Quantitative Biomedical Optics
Based on physical science principles, Quantitative Biomedical Optics covers theory, instrumentation, methods and applications, with practical exercises and problem sets.
Introduction to Forensic Science
Introduction to Forensic Science: The Science of Criminalistics is a textbook that takes a unique and holistic approach to forensic science. This book focuses on exploring the underlying scientific concepts as presented at the introductory college and senior high school levels. Chapters introduce readers to each of the important areas of forensic science, grouping chapters together by discipline and following a logical progression and flow between chapters. This systematically allows students to understand the fundamental scientific concepts, recognize their various applications to the law and investigations, and discern how each topic fits broadly within the context of forensic science. The...
Microscopy Applied to Biophotonics
Biophotonics and microscopy are highly inter-related fields in terms of both technological development and biomedical applications. Recent advances in microscopy have been paralleled by new opportunities for biophotonics, including the investigation and manipulation of biological phenomena using light and its application to biomedicine. This book contains papers from the Enrico Fermi International School of Physics on Microscopy Applied to Biophotonics, held in Varenna, Italy, in July 2011. The lectures spanned the basic science of imaging, through advanced microscopy techniques, to the state-of-the-art in biomedical imaging, and were complemented by seminars from world leaders in biophotonics. Subjects covered include: an overview of biophotonics; fundamentals of microscopy and an introduction to nonlinear microscopy; fluorescence; lasers for biophotonics; and an introduction to ultra-microscopy.
Superresolution Optical Microscopy
This book presents a comprehensive and coherent summary of techniques for enhancing the resolution and image contrast provided by far-field optical microscopes. It takes a critical look at the body of knowledge that comprises optical microscopy, compares and contrasts the various instruments, provides a clear discussion of the physical principles that underpin these techniques, and describes advances in science and medicine for which superresolution microscopes are required and are making major contributions. The text fills significant gaps that exist in other works on superresolution imaging, firstly by placing a new emphasis on the specimen, a critical component of the microscope setup, gi...
Membrane Potential Imaging in the Nervous System and Heart
This volume discusses membrane potential imaging in the nervous system and in the heart and modern optical recording technology. Additionally, it covers organic and genetically-encoded voltage-sensitive dyes; membrane potential imaging from individual neurons, brain slices, and brains in vivo; optical imaging of cardiac tissue and arrhythmias; bio-photonics modelling. This is an expanded and fully-updated second edition, reflecting all the recent advances in this field. Twenty chapters, all authored by leading names in the field, are cohesively structured into four sections. The opening section focuses on the history and principles of membrane potential imaging and lends context to the follo...
