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Stem Cells For Dummies
The first authoritative yet accessible guide to this controversial topic Stem Cell Research For Dummies offers a balanced, plain-English look at this politically charged topic, cutting away the hype and presenting the facts clearly for you, free from debate. It explains what stem cells are and what they do, the legalities of harvesting them and using them in research, the latest research findings from the U.S. and abroad, and the prospects for medical stem cell therapies in the short and long term. Explains the differences between adult stem cells and embryonic/umbilical cord stem cells Provides both sides of the political debate and the pros and cons of each side's opinions Includes medical success stories using stem cell therapy and its promise for the future Comprehensive and unbiased, Stem Cell Research For Dummies is the only guide you need to understand this volatile issue.
Drosophila melanogaster: Practical Uses in Cell and Molecular Biology
Drosophila melanogaster: Practical Uses in Cell and Molecular Biology is a compendium of mostly short technical chapters designed to provide state-of-the art methods to the broad community of cell biologists, and to put molecular and cell biological studies of flies into perspective. The book makes the baroque aspects of genetic nomenclature and procedure accessible to cell biologists. It also contains a wealth of technical information for beginning or advanced Drosophila workers. Chapters, written within a year of publication, make this topical volume a valuable laboratory guide today and an excellent general reference for the future.Key Features* Collection of ready-to-use, state-of-the ar...
Receptor-Receptor Interactions
This new volume of Methods in Cell Biology looks at receptor-receptor interactions, with sections on allosteric and effector interactions, crystallization and modeling, measuring receptor-receptor interactions and oligomerization in individual classes. With cutting-edge material, this comprehensive collection is intended to guide researchers of receptor-receptor interactions for years to come. - Covers sections on allosteric and effector interactions, crystallization and modeling, measuring receptor-receptor interactions and oligomerization in individual classes - Chapters are written by experts in the field - Cutting-edge material
Tetrahymena Thermophila
Tetrahymena thermophila is emerging as a powerful experimental system for functional genetics. The ciliated protozoan offers numerous advantages, not the least of which is the ability to eliminate any specific gene of interest and then to evaluate the effect of that mutation on the living cell. Past investigations with T. thermophila have yielded several key discoveries, including dynein, catalytic RNA, and telomerase. This volume is a comprehensive resource for using Tetrahymena in the laboratory and is aimed at persons already experienced, as well as newcomers to the organism. It covers both the biological background and essential protocols for investigators rapidly turning to Tetrahymena ...
Computational Methods in Cell Biology
Computational methods are playing an ever increasing role in cell biology. This volume of Methods in Cell Biology focuses on Computational Methods in Cell Biology and consists of two parts: (1) data extraction and analysis to distill models and mechanisms, and (2) developing and simulating models to make predictions and testable hypotheses. Focuses on computational methods in cell biology Split into 2 parts--data extraction and analysis to distill models and mechanisms, and developing and simulating models to make predictions and testable hypotheses Emphasizes the intimate and necessary connection with interpreting experimental data and proposing the next hypothesis and experiment
Mitochondria
This title employs biochemical, cell biological, and genetic approaches to study mitochondrial structure, function, and biogenesis. Also of interest are the consequences of impaired mitochondrial function on cells, tissues, and organs. The book is full of step-by-step "how to" methods with sample results, interpretations, and pitfalls. There is a unique set of appendices that include gene catalogs, mtDNA maps, and reagents for probing respiratory chain function. Finally, there are applications of state-of-the art microarray and gene chip technologies. - Isolation of mitochondria from commonly used cells and tissues - Assays for mitochondrial activities, including respiration, ATP production, permeability, protein import, and interactions with the cytoskeleton - Biochemical and optical methods for studying protein-protein interactions in mitochondria - Approaches to studying mitochondrial replication, transcription, and translation - Transmitochondrial technologies - Methods in microassay data analysis
Development of Sea Urchins, Ascidians, and Other Invertebrate Deuterostomes: Experimental Approaches
This book provides a practical guide to experimental methods for studying the development of invertebrate deuterostomes, such as sea urchins, ascidians, hemichordates, and amphioxus. These model organisms are of contemporary and historical importance to the study of developmental biology, particularly genomic research. The chapters provide detailed experimental protocols that cover a broad range of topics in modern experimental methods. Topics covered range from rearing embryos to the care of adult animals, while also presenting the basic experimental methods including light and electron microscopy, used to study gene expression, transgenics, reverse genetics, and genomic approaches. * Covers a wide range of methods, from classical embryology through modern genomics * Discusses animals related to vertebrates, providing a valuable evolutionary perspective * Includes a practical guide to the use of sea urchins in the teaching laboratory
The Zebrafish: Cellular and Developmental Biology
This volume of Methods in Cell Biology, the first of two parts on the subject of zebrafish, provides a comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 1999. This first volume provides state-of-the-art descriptions of novel cellular imaging technologies and methods for culture of zebrafish stem cells, summarizes protocols for analyzingthe development of major organ systems including the central nervous system (CNS), and introduces the use of the zebrafish as a model system for human diseases. - Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls - Illustrates many techiques in full-color - Summarizes the Zebrafish Genome Project
Correlative Light and Electron MIcroscopy
The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM. Covers many areas of CLEM by the best international scientists in the field Includes a brief history and overview on CLEM methods
