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At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). This book focuses on primer design, which is critical to both the efficiency and the accuracy of the PCR. With intricate descriptions of basic approaches as well as specialized methods, "PCR Primer Design" is an exceptional reference for all those involved in studying the genome.
This book offers an essential guide to RNA activation (RNAa), an emerging and fascinating new field. RNAa is a small RNA-guided and Argonaute-dependent gene regulation phenomenon in which promoter-targeted short double-stranded RNAs (dsRNAs) induce target gene expression at the transcriptional level. It occurs primarily in the nucleus and can be mediated by artificially designed short duplex RNAs that target regulatory sequences (e.g., promoters, genes’ 3’ termini and enhancers) and naturally occurring small RNAs (e.g., miRNAs and C. elegans 22G-RNAs). With contributions from internationally respected RNA experts, this book provides comprehensive coverage of different RNAa mechanisms and...
Sidorio, fuelled by grief and revenge, is intent on becoming King of the Vampirates and building a new empire to bring terror to the oceans. He faces growing opposition from both the Pirate Federation, including Vampirate Assassin Cheng Li, and the Nocturnals - the more benign vampirate realm - led by Mosh Zu and Lorcan Furey. Both the pirates and the Nocturnals are forced to raise their game in response to the new and urgent threat from Sidorio and the renegade Vampirates. Twins Grace and Connor Tempest, still ricocheting from the recent discovery of their true parenthood and its explosive implications, are thrust deep into the heart of the conflict. Old foes and allies are thrown together in unexpected ways and, as the stakes rise higher than ever before, Grace and Connor find their alliances shifting in ways no-one could ever have possibly foreseen…
Who will govern China after Jiang Zemin? What path will its new leaders chart in the early years of the twenty-first century? Drawing upon a wealth of both quantitative and qualitative data on the so-called fourth generation of leaders_those who were young during the Cultural Revolution_Cheng Li shows that this group is more diversified than previous generations in formative experiences, political solidarity, ideological conviction, and occupational background. The author explores the contradictions between these emerging leaders and their non-elite peers who were barred from education during the Mao era and now often are unemployed and disenchanted. The book concludes with the intriguing notion that this generation of leaders may have a better understanding of its peersO concerns and therefore may make the regime more accountable to its people, thus contributing to, rather than opposing, democratic development.
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In the rapid development of global economics, energy, environmental & ecosystem are recognized as important factors for sustainable development in human society. The application of measurement and control technology also play a very important role in the utilization and protection of energy and the environment.2015 International Conference on Energy, Environmental & Sustainable Ecosystem Development (EESED 2015) is a multidisciplinary international conference that provides a platform for scientists, engineers and researchers worldwide to share their ideas and present solutions to energy, environmental & sustainable ecosystem development issues.
Conor and Grace are twins, recently orphaned after their widowed father's death. Rather than being adopted by the town's busy-bodies, they decide to set sail in their father's lastpossession, his sailing boat. But a vicious storm sees their boat capsize and the twins are separated. Two mysterious ships sail to their rescue - each picking up one twin before disappearing into the mist. Conor wakes to find himself on a pirate ship and is soon being trained up with a cutlass. Meanwhile Grace finds herself locked in a darkened room, as the vampirates wait for night to fall and their feasting to begin... Determined to find each other, yet intrigued by their new shipmates, the twins are about to embark on the biggest adventure of their lives...
Successfully fighting cancer starts with understanding how it begins. This thoroughly revised 3rd Edition explores the scientific basis for our current understanding of malignant transformation and the pathogenesis and treatment of cancer. A team of leading experts thoroughly explain the molecular biologic principles that underlie the diagnostic tests and therapeutic interventions now being used in clinical trials and practice. Incorporating cutting-edge advances and the newest research, the book provides thorough descriptions of everything from molecular abnormalities in common cancers to new approaches for cancer therapy. Features sweeping updates throughout, including molecular targets for the development of anti-cancer drugs, gene therapy, and vaccines...keeping you on the cutting edge of your specialty. Offers a new, more user-friendly full-color format so the information that you need is easier to find. Presents abundant figures-all redrawn in full color-illustrating major concepts for easier comprehension. Features numerous descriptions of the latest clinical strategies-helping you to understand and take advantage of today’s state-of-the-art biotechnology advances.
This is the proceedings of the International Conference on Intelligent Computing, ICIC 2006, Kunming, China, August 2006. The book presents 165 revised full papers, carefully chosen and reviewed, organized in topical sections on fuzzy systems, fuzzy-neuro-evolutionary hybrids, supervised, unsupervised and reinforcement learning, intelligent agent and Web applications, intelligent fault diagnosis, natural language processing and expert systems, natural language human-machine interface using artificial neural networks, and intelligent financial engineering.
(Bayreuth University, Germany), Jennie Si (Arizona State University, USA), and Hang Li (MicrosoftResearchAsia, China). Besides the regularsessions andpanels, ISNN 2008 also featured four special sessions focusing on some emerging topics.