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Cell-Free Protein Synthesis
The Nobel Prize in Medicine 1968 for interpretation of the genetic code and its function in protein synthesis and in Chemistry 2009 for studies of the structure and function of the ribosome highlighted the ground-breaking experiment performed on May 15, 1961 by Nirenberg and Matthaei and their principal breakthrough on the creation of "cell-free protein synthesis (CFPS) system". Since then the continuous technical advances have revitalized CFPS system as a simple and powerful technology platform for industrial and high-throughput protein production. CFPS yields exceed grams protein per liter reaction volume and offer several advantages including the ability to easily manipulate the reaction components and conditions to favor protein synthesis, decreased sensitivity to product toxicity, batch reactions last for multiple hours, costs have been reduced orders of magnitude, and suitability for miniaturization and high-throughput applications. With these advantages, there is continuous increasing interest in CFPS system among biotechnologists, molecular biologists and medical or pharmacologists.
Enzyme Inactivation in Food Processing
Enzyme inactivation in fruits and vegetables is of utmost importance regarding food quality during storage. This new volume explores important emerging technologies for the inactivation of enzymes in the design and preservation of food. The book covers the basic concepts and chemical methods and then introduces novel processing technologies for inactivating food enzymes. The new technologies are many: pulsed electric field, ultraviolet and light-emitting diodes, ohmic heating, dense-phased carbon dioxide, cold plasma, ultrasonication, microwave processing, radiofrequency, extraction, and others. The volume also looks at the design of nutraceutical-based functional foods, specific foods for gut-microbiodata, the use of omega-3 fatty acids to fortify food products, and the characteristics of dairy-based dry powders, and characteristics of millet starches. It also considers the role of the bioactive compounds and metal ions for catalases secreted by medicinal plants and mushrooms for enzyme inactivation and biosensing, along with the role of bionanomaterials in nanoencapsulation and catalysis.
Sustainable Green Nanomaterials
Green nanomaterials are in great demand as natural substitutes to conventional chemical-based, eco-toxic materials in fabricating numerous eco-friendly products that are biodegradable and biocompatible and eco-benign for a plethora of applications in biomedicine, textiles, agriculture, and many other industries. This new book, Sustainable Green Nanomaterials: Synthesis, Characterization, and Engineering Applications, presents an overview of fabrication, testing, and utility of green nanomaterials in sustainable agriculture, smart drug delivery, wastewater treatment, and healthcare. The first section of the book is devoted to the synthesis, characterization, and general applications of green ...
Antimicrobial Dressings
Antimicrobial Dressings: The Wound Care Applications explores the literature surrounding the catalytic behavior of proteolytic enzymes immobilized together with nanoparticles. As numerous applications using proteolytic enzymes for debridement, silver as antibiotic and nanoparticles for enzyme immobilization were developed in the last years, this book explores interdisciplinary information combining nanotechnology, biotechnology and medicine and how it's still in early stages. The book adopts a holistic approach in a lifecycle context to evaluate their final feasibility, including industrial exploitability without disregard of the potential risks of enzymes and nanomaterials to human health a...
Advances in Bioremediation and Phytoremediation for Sustainable Soil Management
This edited volume deals with the understanding of the issues concerned with the pollution caused by toxic elements and heavy metals and their impacts on the different agro-ecosystems as well as the techniques involved in sustainable remediation and amelioration of polluted soils. Furthermore, the book is a detailed comprehensive account for the treatment technologies from unsustainable to sustainable which includes chapters prepared by professionals with expertise in environmental microbiology, biotechnology, bioremediation, and environmental engineering. It focuses on the characterization, reclamation, bioremediation, and phytoremediation of polluted soils. The research presented also highlights some of the significantly important plant and microbial species involved in remediation, the physiology, biochemistry, and the mechanisms of remediation by various plants and microbes, and suggestions for future improvement of bioremediation technology. It offers insights into the current focus and recent advances in bioremediation and green technology applications for sustainable soil management.
Microbial Exopolysaccharides as Novel and Significant Biomaterials
This book examines the commercial role of various microbial polysaccharides and recent advances in their production. Offering an overview of the physiological role, biosynthetic pathways and regulatory mechanisms, it also explores the current challenges regarding bioprocessing for the production of polysaccharides.
Cytogenetics
This edited book, Cytogenetics - Classical and Molecular Strategies for Analysing Heredity Material, presents recent advances in the field of cytogenetics, paying special attention to methodological achievements developed worldwide that have driven the field forward. The contributors clearly discuss several concepts and approaches useful for understanding chromosomal structure and function at its various levels, highlighting chromosomes as visible carriers of heredity material.
Sustainable Nanomaterials for Biomedical Engineering
In recent years, nanomaterials have become one of the most dynamic exploration fields in the areas of engineering, technology, and science. This new volume focuses on the use of various bionanomaterials that can be introduced into the body as clinical devices for various medical purposes. The book also provides examples of cost-effective, sustainable alternatives to traditional medical procedures. The volume discusses how these materials have diverse applications in the biomedical fields, such as for cancer treatment, for orthopedic joint replacements, for medical diagnosis, for making bone plates, for wound healing, for nerve regeneration, for breast implants, in dental procedures, and so on. In addition, the book also covers some nonbiomedical applications of nanobiomaterials, such as, for example, to grow cells in a culture medium, as a blood protein test in laboratories, etc.
Synthetic Genomics
The current advances in sequencing, data mining, DNA synthesis, cloning, in silico modeling, and genome editing have opened a new field of research known as Synthetic Genomics. The main goal of this emerging area is to engineer entire synthetic genomes from scratch using pre-designed building blocks obtained by chemical synthesis and rational design. This has opened the possibility to further improve our understanding of genome fundamentals by considering the effect of the whole biological system on biological function. Moreover, the construction of non-natural biological systems has allowed us to explore novel biological functions so far not discovered in nature. This book summarizes the current state of Synthetic Genomics, providing relevant examples in this emerging field.
Recent Advances in Plant Biotechnology and Its Applications
This book is divided into five sections. The first section deals with the methodology and bioresource generation, techniques related to genetic engineering, and gene transfer to the nuclear genome and chloroplast genome. The new techniques of genome profiling and gene silencing are also presented. The second section of the book covers the classical aspect of plant biotechnology viz. tissue culture and micropropagation. Use of genetic engineering via Agrobacterium and direct transfer of DNA through particle bombardment to develop transformed plants in Artemisia, castor and orchids, and production of recombinant proteins in plant cells have been dealt with in the third section. The fourth section addresses the abiotic and biotic stress tolerance in plants. The basic biology of some of the stress responses, and designing plants for stress tolerance is discussed in this section. The fifth section examines medicinal plants and alkaloid production.
