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The Yeast Two-hybrid System
This volume, part of the Advances in Molecular Biology series, presents work by pioneers in the field and is the first publication devoted solely to the yeast two-hybrid system. It includes detailed protocols, practical advice on troubleshooting, and suggestions for future development. In addition, it illustrates how to construct an activation domain hybrid library, how to identify mutations that disrupt an interaction, and how to use the system in mammalian cells. Many of the contributors have developed new applications and variations of the technique.
Cell Biology
This four-volume laboratory manual contains comprehensive state-of-the-art protocols essential for research in the life sciences. Techniques are presented in a friendly step-by-step fashion, providing useful tips and potential pitfalls. The important steps and results are beautifully illustrated for further ease of use. This collection enables researchers at all stages of their careers to embark on basic biological problems using a variety of technologies and model systems. This thoroughly updated third edition contains 165 new articles in classical as well as rapidly emerging technologies. Topics covered include: Cell and Tissue Culture: Associated Techniques, Viruses, Antibodies, Immunocyt...
Delphi Complete Works of Jules Verne (Illustrated)
Jules Verne is a paramount literary figure, whose pioneering works have entertained readers for over a hundred years, laying the foundations of modern science fiction. Verne’s influence extends far beyond the realms of literature into the world of science and technology, where he inspired generations of scientists, inventors and explorers. This eBook offers the most complete collection ever compiled of Verne’s work in English translation, with numerous illustrations, rare novels and informative introductions. (Version 4) * Beautifully illustrated with images relating to Verne’s life and works * Concise introductions to the novels and other texts * 50 novels in English translation, with...
How to Influence and Persuade
Everyone operating in business needs to understand how they should be using both influence and persuasion to make an impact, deliver outstanding results and really get ahead. This brand new edition of How to Influence, is structured into three easy-to-access sections that will show you how to create willing allies across your organisation, turn potential crises into career defining opportunities, turn disagreement into consent, win key battles without fighting, push your agenda forward with authority, achieve more and stay in control. Author Jo Owen is highly regarded as a world-leading expert in business practise and skills.
Computational Biology
This book presents a selection of revised full papers accepted for presentation at the First International Conference on Biology, Informatics, and Mathematics, JOBIM 2000, held in Montpellier, France, in May 2000. The 13 papers included in the book were selected after two rounds of reviewing and revision from a total of 67 submissions. Among the topics addressed are algorithms, comparative genomics, evolution, phylogeny, databases, knowledge processing, genome anotation, graph theory, combinatorial mathematics, macromolecular structures, RNA and proteins, metabolic pathways and regulatory networks, and statistics and classification.
Homo Conexus
Homo Conexus is the type of people we become as we venture forward into the network society. From the networks in our brains to the networks on our computers, networks are changing the way we think about ourselves and the world. Homo Conexus helps you make sense of this new age of information and networks.
The Necessity of Strangers
A counterintuitive approach to fostering greater innovation, collaboration, and engagement Most of us assume our success relies on a network of friends and close contacts. But innovative thinking requires a steady stream of fresh ideas and new possibilities, which strangers are more likely to introduce. Our survival instincts naturally cause us to look upon strangers with suspicion and distrust, but in The Necessity of Strangers, Alan Gregerman offers the provocative idea that engaging with strangers is an opportunity, not a threat, and that engaging with the right strangers is essential to unlocking our real potential. The Necessity of Strangers reveals how strangers challenge us to think d...
Data Management of Protein Interaction Networks
Current PPI databases do not offer sophisticated querying interfaces and especially do not integrate existing information about proteins. Current algorithms for PIN analysis use only topological information, while emerging approaches attempt to exploit the biological knowledge related to proteins and kinds of interaction, e.g. protein function, localization, structure, described in Gene Ontology or PDB. The book discusses technologies, standards and databases for, respectively, generating, representing and storing PPI data. It also describes main algorithms and tools for the analysis, comparison and knowledge extraction from PINs. Moreover, some case studies and applications of PINs are also discussed.
Rethinking Cancer
Leading scientists argue for a new paradigm for cancer research, proposing a complex systems view of cancer supported by empirical evidence. Current consensus in cancer research explains cancer as a disease caused by specific mutations in certain genes. After dramatic advances in genome sequencing, never before have we known so much about the individual cancer cell--and yet never before has it been so unclear what to do with this knowledge. In this volume, leading researchers argue for a new theory framework for understanding and treating cancer. The contributors propose a complex systems view of cancer, presenting conceptual building blocks for a new research paradigm supported by empirical...
