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Biophysical, Chemical, and Functional Probes of RNA Structure, Interactions and Folding: Part B
This MIE volume provides laboratory techniques that aim to predict the structure of a protein which can have tremendous implications ranging from drug design, to cellular pathways and their dynamics, to viral entry into cells. - Expert researchers introduce the most advanced technologies and techniques in protein structure and folding - Includes techniques on tiling assays
The Chemical Biology of Nucleic Acids
With extensive coverage of synthesis techniques and applications, this text describes chemical biology techniques which have gained significant impetus during the last five years. It focuses on the methods for obtaining modified and native nucleic acids, and their biological applications. Topics covered include: chemical synthesis of modified RNA expansion of the genetic alphabet in nucleic acids by creating new base pairs chemical biology of DNA replication: probing DNA polymerase selectivity mechanisms with modified nucleotides nucleic-acid-templated chemistry chemical biology of peptide nucleic acids (PNA) the interactions of small molecules with DNA and RNA the architectural modules of f...
Functional Nucleic Acids for Analytical Applications
Nature has long used nucleic acid aptamers and enzymes for regulatory activities, such as the recently discovered “riboswitches” involved in gene expression. The existence of a large array of natural and artificial functional nucleic acids has generated tremendous enthusiasm and new opportunities for molecular scientists from diverse disciplines to devise new concepts and real applications that take advantage of those nucleic acids for sensing and other analytical applications. This book provides a timely and comprehensive overview of recent advances in the field, from leading experts in biology, chemistry, and engineering. A variety of topics are covered, from fundamentals of functional nucleic acids, to their applications as sensors, to nanotechnologies; as well as integration of functional nucleic acids into practical analytical systems.
Magnetism: A Supramolecular Function
Molecular magnetism is a new field of research dealing with the synthesis and study of the physical properties of molecular assemblies involving open-shell units. It is essentially interdisciplinary, joining together organic, organometallic and inorganic chemists, as well as theoreticians, physicists and materials scientists. At the core of research into molecular magnetism lie design and synthesis of new molecular assemblies exhibiting bulk properties such as long-range magnetic ordering or bistability with an hysteresis effect, which confers a memory effect on the system. In such terms, magnetism may be considered a supramolecular function. The first eight contributions to this volume present the state of the art in organic supramolecular chemistry, emphasising interlocked systems and molecular trees. The following six articles are devoted to molecular materials constructed from organic radicals and transition metal units. Molecular bistability is then focused on, followed by metal-organic and coordination magnetic materials. A new approach to nano-sized particles closes the work.
Biocatalysis and Agricultural Biotechnology: Fundamentals, Advances, and Practices for a Greener Future
This new volume, Biocatalysis and Agricultural Biotechnology: Fundamentals, Advances, and Practices for a Greener Future, looks at the application of a variety of technologies, both fundamental and advanced, that are being used for crop improvement, metabolic engineering, and the development of transgenic plants. The science of agriculture is among the oldest and most intensely studied by mankind. Human intervention has led to manipulation of plant gene structure for the use of plants for the production of bioenergy, food, textiles, among other industrial uses. A sound knowledge of enzymology as well as the various biosynthetic pathways is required to further utilize microbes as sources to p...
Directory of Graduate Research
Faculties, publications and doctoral theses in departments or divisions of chemistry, chemical engineering, biochemistry and pharmaceutical and/or medicinal chemistry at universities in the United States and Canada.
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Enzymes for Solving Humankind's Problems
This book presents specific key natural and artificial systems that are promising biocatalysts in the areas of health, agriculture, environment and energy. It provides a comprehensive account of the state of the art of these systems and outlines the significant progress made in the last decade using these systems to develop innovative, sustainable and environmentally friendly solutions. Chapters from expert contributors explore how natural enzymes and artificial systems tackle specific targets such as: climate change, carbon footprint and economy and carbon dioxide utilisation; nitrogen footprint and fixation and nitrous oxide mitigation; hydrogen production, fuel cells and energy from bacte...
Phage Display
Phage-display technology has begun to make critical contributions to the study of molecular recognition. DNA sequences are cloned into phage, which then present on their surface the proteins encoded by the DNA. Individual phage are rescued through interaction of the displayed protein with a ligand, and the specific phage is amplified by infection of bacteria. Phage-display technology is powerful but challenging and the aim of this manual is to provide comprehensive instruction in its theoretical and applied so that any scientist with even modest molecular biology experience can effectively employ it. The manual reflects nearly a decade of experience with students of greatly varying technical expertise andexperience who attended a course on the technology at Cold Spring Harbor Laboratory. Phage-display technology is growing in importance and power. This manual is an unrivalled source of expertise in its execution and application.
