Welcome to our book review site go-pdf.online!
You may have to Search all our reviewed books and magazines, click the sign up button below to create a free account.
Rapid Cycle Real-Time PCR — Methods and Applications
Rapid Cycle Real-Time PCR is a powerful analytical tool with broad application for the basic and applied life sciences. Compared with conventional PCR technology, Rapid Cycle Real-Time PCR is faster, has greater specificity, and is more easily adaptable for a variety of diagnostic tests, including qualitative, quantitative and mutation detection assays. This book provides general overviews of this technology for use in the clinical microbiology laboratory as well as specific diagnostic protocols for the detection of viral, bacterial and fungal pathogens and genetically modified organisms in human specimens and foodstuffs. All of these protocols have been developed, verified, and validated by experts in the field and should be of great interest for clinical microbiologists, pathologists, laboratory technologists as well as practicing physicians.
Hemostasis and Thrombosis Protocols
Laboratory studies in hemostasis have traditionally focused on abn- malities of platelet function or the quantitative and qualitative disorders that affect the proteins involved in blood coagulation. However, over the last 10 years there has been an explosion in our understanding of the molecular bases that underlie many of the inherited and acquired disorders of hemostasis. Many of these disorders are now routinely diagnosed and assessed by methods that involve genotypic analysis. Indeed in the late 1990s the distinction between molecular methods for research and for routine diagnosis is becoming incre- ingly blurred. The techniques and approaches that are used in hemostasis are manifold an...
Cytotoxic Drug Resistance Mechanisms
There is now a range of cytotoxic drugs that have considerable clinical usefulness in producing responses in tumors and even, in a small proportion of cases, cure. However, the acquisition of drug resistance is a major clinical problem and is perhaps the main limiting factor in successful treatment of cancer. Thus, a tumor initially sensitive to chemotherapy will, in the majority of cases, eventually recur as a resistant tumor, which will then progress. Much of our understanding of drug resistance mechanisms comes from the study of tumor cell lines grown in tissue culture. We now understand many of the - lecular mechanisms that can lead to a cell acquiring resistance to antic- cer drugs; how...
Vascular Disease
Molecular biology has revolutionized research into vascular disease. Over the past 20 years molecular techniques have enabled us to both elucidate - lecular mechanisms in vascular disease and identify appropriate therapies. The vast explosion in technical knowledge and the array of protocols that become more advanced and intricate by the day lead us into new and exciting areas of research that were previously unobtainable. Vascular Disease: Molecular Biology and Gene Transfer Protocols - scribes today’s most powerful molecular methods for the investigation of the pathogenesis of vascular disease. The protocols are highly detailed, allowing beginners who have little experience in either vas...
HIV Protocols
The worldwide impact of infection with human immunodeficiency virus type 1 (HIV- is reflected in the cumulative number ofHIV- 1 infections, which is now predicted to exceed 40 million by the year 2000---equivalent to the n- ber of humans who perished in World War II. The medical and scientific - sponse to the HIV-1 pandemic has steadily grown since its recognition in 1981. The outlay by the United States alone for HIV research funded by the National Institutes of Health in 1997 was $1. 4 billion. Laboratory-based HIV research has brought together academic clinicians, retrovirologists, molecular biologists, and immunologists in the formation of research teams attempting to dissect the viral a...
Pancreatic Cancer
Pancreatic ductal adenocarcinoma is the fourth leading cause of cancer death in the United States. Annually approximately 30,000 Americans are diagnosed with the disease and most will die from it within five years. P- creatic ductal adenocarcinoma is unique because of its late onset in age, high mortality, small tumor samples infiltrated with normal cells, and a lack of both early detection and effective therapies. Some of these characteristics have made studying this disease a challenge. Pancreatic cancer develops as a result of the accumulation of genetic alterations in cancer-causing genes, such as the oncogenes and the tumor-s- pressor genes. In the last decade, major progress has been m...
Diagnostic Virology Protocols
A collection of cutting-edge techniques for detecting most of the major viruses that afflict mankind, including influenza, hepatitis, herpes, polio, mumps, HIV, and many more. The techniques are well-tested, easily reproducible, and readily employ all the new technologies-PCR, RIA, ELISA, and latex-agglutination-that have revolutionized the field. These methods not only make it possible to do the necessary analysis in hours instead of days, but can also be automated in a laboratory havng only low levels of biological containment. Frequently, the protocols for viruses causing human diseases can be adapted to similar viruses of veterinary importance. Through its state-of-the-art methods a physician can, for the first time, determine early in a viral infection which antiviral drug should be used and minimize the period of treatment to avoid unnecessary side effects.
Microarrays in Clinical Diagnostics
Leading academic and industrial investigators surveys the world of microarray technology, describing in step-by-step detail diverse DNA and protein assays in clinical laboratories using state-of-the-art technologies. The advanced tools and methods described are designed for mRNA expression analysis, SNP analysis, identification, and quantification of proteins, and for studies of protein-protein interactions. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Point-of-care testing
The underlying technology and the range of test parameters available are evolving rapidly. The primary advantage of POCT is the convenience of performing the test close to the patient and the speed at which test results can be obtained, compared to sending a sample to a laboratory and waiting for results to be returned. Thus, a series of clinical applications are possible that can shorten the time for clinical decision-making about additional testing or therapy, as delays are no longer caused by preparation of clinical samples, transport, and central laboratory analysis. Tests in a POC format can now be found for many medical disciplines including endocrinology/diabetes, cardiology, nephrolo...
