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This detailed new edition provides a comprehensive collection of protocols applicable to all members of the Coronavirinae sub-family currently and that are also transferrable to other fields of virology. Beginning with a section on detection, discovery, and evolution, the volume continues with coverage of propagation and titration of coronaviruses, genome manipulation, study of virus-host interactions, as well as imaging coronavirus infections. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Coronaviruses: Methods and Protocols, Second Edition serves as a valuable guide to researchers working to identify and control viruses with increased potential to cross the species barrier and to develop the diagnostics, vaccines, and antiviral therapeutics that are required to manage future outbreaks in both humans and animals.
This book presents an Asian perspective on how the treatment of colorectal cancer can be optimized and standardized in ways that take into account technological advances and the trend towards individually tailored therapy. Readers will find careful, well-illustrated descriptions of the standard surgical techniques for rectal cancer and colon cancer that have contributed to recent improvements in 5-year survival rates in the Asia-Pacific region, where the incidence of colorectal cancer has been rising alarmingly due to lifestyle changes. The vital role now being played by minimally invasive laparoscopic and robotic options receives detailed scrutiny. Extent and timing of surgery, patient safety, risk of complications, and unresolved issues are all discussed. Furthermore, the use of surgery within the context of multimodal management including chemotherapy and radiotherapy is explained and an integrated approach for stage IV and recurrent disease is described. The book will serve as a valuable reference for young surgeons who are in training, experienced practitioners who want to enhance their knowledge and skills, and all others who wish to learn about this field.
After the tragic loss of his younger brother, Jono Lineen experienced walking’s regenerative power firsthand. Grief-stricken and adrift, he set off on a 2700-kilometre solo trek across the Himalayas. He walked for months until his legs ached and feet blistered, and by the end of the expedition something had changed in him. He was stronger – not just physically, but psychologically and emotionally. What had happened? What had given him this feeling of peace; joy even? Determined to find out, he began investigating the science and history of walking and running, and discovered that there were fascinating reasons for his metamorphosis. Now, weaving together his own remarkable personal stories with research, Lineen reveals for the first time the powerful effect that even the shortest strolls can have on us. And why walking is what we’re made to do; it is our perfect motion.
This volume offers researchers and practitioners new perspectives on applied theatre work, exploring the relationship between applied theatre and its intent, success and value. Applied theatre is a well-established field focused on the social application of the arts in a range of contexts including schools, prisons, residential aged care and community settings. The increased uptake of applied theatre in these contexts requires increased analysis and understanding of indications of success and value. This volume provides critical commentary and questions regarding issues associated with developing, delivering and evaluating applied theatre programs. Part 1 of the volume presents a discussion of the ways the concept of change is presented to and by funding bodies, practitioners, participants, researchers and policy makers to discover and analyse the relationships between applied theatre practice, transformative intent, and evaluation. Part 2 of the volume offers perspectives from key authors in the field which extend and contextualize the discussion by examining key themes and practice-based examples.
Nicola Pasic and Ante Trumbic: The book will provide the first parallel biographies of two key Yugoslav politicians of the early 20th century: Nikola Pasic, a Serb, and Ante Trumbic, a Croat. It will also offer a brief history of the creation of Yugoslavia (initially known as the Kingdom of Serbs, Croats, and Slovenes), internationally accepted at the Paris Peace Conference of 1919-20 (at the Treaty of Versailles). Such an approach will fill two major gaps in the literature - scholarly biographies of Pasic and Trumbic are lacking, while Yugoslavia's formation is due a reassessment - and to introduce the reader to the central question of South Slav politics: Serb-Croat relations. Pasic and Trumbic's political careers and their often troubled relationship in many ways perfectly epitomize the wider Serb-Croat question.
Tackles the thorny issue of the disappearance of Montenegro as a sovereign state in the course of and as a result of the First World War. This book investigates the ambiguous and often troubled relationship between two "Serb states," Montenegro and Serbia. It examines the politics and power plays of Serbs, Montenegrins, and others.