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This book presents a wide range of biotechnological methods for application in soil microbiology analysis, including all essential methods involving molecular biology, immunology, microbiology, and structural biology, such as transcriptome analysis, RNAi technology, molecular matchmaking, RAPD, T-RFLP and FT/MS. The techniques and procedures presented here offer practical guides for immediate use in the laboratory. This volume will be of use both to the first-timer and to the experienced scientist.
Human activities have dramatically changed the composition and organisation of soils. Industrial and urban wastes, agricultural application and also mining activities resulted in an increased concentration of heavy metals in soils. How plants and soil microorganisms cope with this situation and the sophisticated techniques developed for survival in contaminated soils is discussed in this volume. The topics presented include: the general role of heavy metals in biological soil systems; the relation of inorganic and organic pollutions; heavy metal, salt tolerance and combined effects with salinity; effects on abuscular mycorrhizal and on saprophytic soil fungi; heavy metal resistance by streptomycetes; trace element determination of environmental samples; the use of microbiological communities as indicators; phytostabilization of lead polluted sites by native plants; effects of soil earthworms on removal of heavy metals and the remediation of heavy metal contaminated tropical land.
For this third volume of the series Soil Biology, internationally renowned scientists shed light on the significant roles of microbes in soil. Key topics covered include: bioerosion, humification, mineralization and soil aggregation; Interactions in the mycorrhizosphere; microbes and plant nutrient cycling; Microbes in soil surface or toxic metal polluted soils; Use of marker genes and isotopes in soil microbiology, and many more.
Mycotoxins are made by different biosynthetic pathways, and they have an extremely wide range of pharmacological effects. This book will update readers on several cutting-edge aspects of mycotoxin research, including topics such as: new analytical methods for detection; the adoption of an ancient Mexican process for detoxification of aflatoxins; mycotoxin management in Ireland, Lithuania and South America; mycotoxin reduction through plant breeding and integrated management practices; and natural aflatoxin inhibitors from medicinal plants. Further contributions examine ochratoxins, selected trichothecenes, zearalenone, and aflatoxin-like gene clusters, as well as sclerotial development in Aspergillus flavus and A. parasiticus. Of particular interest are the chapters on the potential use of mycotoxins as bioweapons. This book will stimulate new thinking on the need to develop therapeutic as well as preventative interventions to reduce the toxicological threat of mycotoxins.
This book is perfectly timed for the worldwide explosion of interest in mycorrhizal research. With a strong emphasis on the latest findings in genetics and molecular biology, it contains all current information and speculation on the structure, function and biotechnological applications of mycorrhizas.
Symbiotic Fungi – Principles and Practice presents current protocols for the study of symbiotic fungi and their interactions with plant roots, such as techniques for analyzing nutrient transfer, ecological restoration, microbial communication, and mycorrhizal bioassays, AM inoculum procedures and mushroom technology. The protocols offer practical solutions for researchers and students involved in the study of symbiotic microorganisms. The volume will be of great use for basic research, biotechnological applications, and the development of commercial products.
In response to low iron availability in the environment most microorganisms synthesize iron chelators, called siderophores. Bacteria and fungi produce a broad range of structurally diverse siderophores, all of which show a very high affinity for ferric ions. This book presents an up-to-date overview of the chemistry, biology and biotechnology of these iron chelators. Coverage ranges from an introductory chapter to siderotyping to applications in human and plant health.
Ectomycorrhizal fungi play multifunctional roles during symbioses with higher plants. They can serve as bioprotectors, biofertilizers, bioremediators and stress indicators. Further, they are the true “mycoindicators” of forest ecosystems, where an enormous diversity of ectomycorrhizal fungi can be found. Some ectomycorrhizal fungi also produce edible sporocarps, i.e., fruiting bodies, which are important for the food industry. Ectomycorrhizal fungi also produce various metal chelating molecules, which are of remarkable biotechnological significance and which also secrete useful secondary metabolites. Molecular approaches are required for the identification and differentiation of fungi forming symbioses with higher plants, while molecular tools are important to understand how genes are expressed during symbiosis with higher plants. Students, researchers and teachers of botany, mycology, microbiology, forestry, and biotechnology will find a valuable source of information in this Soil Biology volume.
Soil enzymes are one of the vital key mediators involved in nutrient recycling and the decomposition of organic matter and thereby in maintaining soil quality and fertility. This Soil Biology volume covers the various facets of soil enzymes, such as their functions, biochemical and microbiological properties and the factors affecting their activities. Enzymes in the rhizosphere, in forest soils, and in volcanic ash-derived soils are described. Soil enzymes covered include phosphohydrolases, lignocellulose-degrading enzymes, phenol oxidases, fungal oxidoreductases, keratinases, pectinases, xylanases, lipases and pectinases. Several chapters treat the soil enzymatic activities in the bioremediation of soils contaminated with pesticides and pollutants such as oil, chlorinated compounds, synthetic dyes and aromatic hydrocarbons. The role of soil enzymes as bioindicators is a further important topic addressed.
This is the first work to focus on microbes in gut systems of soil animals. Beginning with an overview of the biology of soil invertebrates, the text turns to the gut microbiota of termites, which are important soil processors in tropical and subtropical regions. Coverage extends to intestinal microbiota of such other litter decomposers as earthworms, springtails, millipedes, and woodlice. Thoroughly illustrated, including color photographs.