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Cells and Culture
Regeneration of tissue to replace damaged or injured tissue is the goal of t- sue engineering. Biomaterials like polyglycolic acid, collagen and small-intestinal submuscosa provide a temporary scaffold to guide new tissue growth and or- nization. Typically, they need to be biodegradable, showing good cell atta- ment and proliferation and they should possess appropriate mechanical properties (Kim et al. , 2000). Synthetic polymers ful ll most of these requirements but lack cell-adhesion peptides on their surface to enhance cell attachment. Ce- adhesion peptides are present in ECM proteins like collagen and elastin. Thus a synthetic polymer coated with ECM proteins would result in a scaffold t...
Animal Cell Technology Meets Genomics
The 18th ESACT meeting was celebrated in Granada (Spain) in May 2003, and was entitled "Animal Cell Technology Meets Genomics", in order to reflect that the emerging technologies in the area of genomics, proteomics and other "-omics"-type disciplines will provide key technological assets to increase knowledge and open new horizons in animal cell technology. During the meeting a variety of top-class emerging technologies were presented together with the lastest advances in more mature industrial areas. The meeting was opened by a first session devoted to the understanding of basic cellular mechanisms, and four sessions focused on applied aspects of animal cell technology: Cell-based therapies and gene-based therapies, target discovery and biopharmaceuticals. The Granada Meeting has also seen a special focus on forefront industrial case studies. The spirit and scientific excellence of the 18th ESACT meeting is now reflected in different chapters of the book. The book presents, in form of short papers, a high number of the contributions to the meeting, and has been prepared with the aim to provide a relevant reference of the current research efforts in Animal Cell Technology.
Animal Cell Technology: Basic & Applied Aspects
Animal cell technology is a growing discipline of cell biology which aims not only to understand structures, functions and behaviors of differentiated animal cells but also to uncover their abilities for industrial and medical purposes. The goal of animal cell technology includes clonal expansion of differentiated cells with useful abilities, optimization of their culture conditions on the industrial scale, modulation of their ability in order efficiently to produce medically and pharmaceutically important proteins, and application of animal cells to gene therapy and formation of artificial organs. This Volume gives the readers a complete review of the present state of the art in Japan, a country where this field is well advanced, as well as in Asia, Europe and the United States. The Proceedings will be useful for cell biologists, biochemists, molecular biologists, biochemical engineers and those in other disciplines related to animal cell culture, working in academic environments as well as in the biotechnology and pharmaceutical industries.
Cell Culture Engineering IV
Cell Culture Engineering IV, Improvements of Human Health covers the latest approaches to improving the cell host through improved understanding of the molecular biology, the development of novel vaccines, approaches to bioreactor design and operation, monitoring techniques in process control and quality related topics. The work was carefully put together as one result of the Cell Culture Engineering IV Meeting held in San Diego, U.S.A. in 1994, however, the book may not be perceived as a proceedings volume - the criteria of the book series apply. For cell biologists, biochemists, molecular biologists, immunologists and other disciplines related to cell culture engineering, working in the academic environment, as well as in (biotechnology or pharmaceutical) industry.
Animal Cell Technology
Animal Cell Technology: Products of Today, Prospects for Tomorrow is a collection of papers that discusses the advancement and future of biotechnology. The book presents a total of 164 materials that are organized into 22 sections. The coverage of the text includes the various methodologies involved in animal cell technology, such as post translational modifications; kinetics and modeling; and measurement and assay. The book also covers product safety and consistency testing; products from animal cells in culture; and apoptosis and cell biology. The text will be of great use to biologists, biotechnicians, and biological engineers. Readers who have an interest in the advancement of biotechnology will also benefit from the book.
Baculovirus
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Taking the Bite out of Rabies
Taking the Bite out of Rabies records the evolution of rabies management and control in Canada.
Gene Therapy Protocols
In Gene Therapy Protocols, Volumes 1 and 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of Production and In Vivo Applications of Gene Transfer Vectors (Volume 1) and Design and Characterization of Gene Transfer Vectors (Volume 2). In this second volume, readers will find a comprehensive resource of current and emerging methods for the processing and characterization of viral and non-viral gene transfer vectors.
Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology
More then 20 years have passed now since the first recombinant protein producing microorganisms have been developed. In the meanwhile, numerous proteins have been produced in bacteria, yeasts and filamentous fungi, as weIl as higher eukaryotic cells, and even entire plants and animals. Many recombinant proteins are on the market today, and some of them reached substantial market volumes. On the first sight one would expect the technology - including the physiology of the host strains - to be optimised in detail after a 20 year's period of development. However, several constraints have limited the incentive for optimisation, especially in the pharmaceutical industry like the urge to proceed quickly or the requirement to define the production parameters for registration early in the development phase. The additional expenses for registration of a new production strain often prohibits a change to an optimised strain. A continuous optimisation of the entire production process is not feasible for the same reasons.
