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Surgical Research in Implant Dentistry
This book explores, in a comprehensive and systematic manner, Surgical Research methodologies to the design and development of investigational studies in the field of implant-based oral rehabilitation. Such studies are linked in a chain binding in vitro, in vivo, ex vivo, and digital evaluations. An ambitious objective consists in exposing how investigators can generate and deliver knowledge by capturing synergy coming from preclinical and clinical trials. Accordingly, throughout the book, translational aspects are closely scrutinized. The opening chapters consider materials, methods and procedures for preclinical and clinical studies in which approaches and models are extensively described....
Post-Processing Techniques for Metal-Based Additive Manufacturing
This book shares insights on post-processing techniques adopted to achieve precision-grade surfaces of additive manufactured metals including material characterization techniques and the identified material properties. Post-processes are discussed from support structure removal and heat treatment to the material removal processes including hybrid manufacturing. Also discussed are case studies on unique applications of additive manufactured metals as an exemplary of the considerations taken during post-processing design and selection. Addresses the critical aspect of post-processing for metal additive manufacturing Provides systematic introduction of pertinent materials Demonstrates post-process technique selection with the enhanced understanding of material characterization methods and evaluation Includes in-depth validation of ultra-precision machining technology Reviews precision fabrication of industrial-grade titanium alloys, steels, and aluminium alloys, with additive manufacturing technology The book is aimed at researchers, professionals, and graduate students in advanced manufacturing, additive manufacturing, machining, and materials processing.
Stalk Structures in Lipid Bilayer Fusion Studied by X-ray Diffraction
The fusion of two biological membranes is an important step in many processes on the cellular and sub-cellular level. Understanding the involved interplay of different lipid species, a specialized protein machinery and water on length scales of few nanometers poses a significant challenge to current structural biology. Among several complementary approaches, one strategy is to study the structural rearrangements of the lipid matrix. As the initial step, lipid bilayers must be forced into close contact to form a non-bilayer intermediate termed a stalk. This has been the subject of numerous theoretical studies and simulations, but experimental data on stalks are largely lacking. Currently, the...
Towards Autonomous Soft Matter Systems
This book focuses on the assembly, organization and resultant collective dynamics of soft matter systems maintained away from equilibrium by an energy flux. Living matter is the ultimate example of such systems, which are comprised of different constituents on very different scales (ions, nucleic acids, proteins, cells). The result of their diverse interactions, maintained using the energy from physiological processes, is a fantastically well-organized and dynamic whole. This work describes results from minimal, biomimetic systems and primarily investigates membranes and active emulsions, as well as key aspects of both soft matter and non-equilibrium phenomena. It is shown that these minimal reconstitutions are already capable of a range of complex behaviour such as nonlinear electric responses, chemical communication and locomotion. These studies will bring us closer to a fundamental understanding of complex systems by reconstituting key aspects of their form and function in simple model systems. Further, they may also serve as the first technological steps towards artificial soft functional matter.
Cone-beam X-ray Phase Contrast Tomography of Biological Samples
Three-dimensional information of entire objects can be obtained by the remarkable technique of computed tomography (CT). In combination with phase sensitive X-ray imaging high contrast for soft tissue structures can be achieved as opposed to CT based on classical radiography. In this work biological samples ranging from micrometer sized single cells over multi-cellular nerve tissue to entire millimeter sized organs are investigated by use of cone-beam propagationbased X-ray phase contrast. Optimization with respect to contrast, resolution and field of view is achieved by addressing instrumentation, sample preparation and phase reconstruction techniques. By using laboratory sources functional soft tissue within the bony capsule of mouse cochleae is visualized in 3D with unprecedented image quality. At synchrotron storage rings the technique reveals more than 1000 axons running in parallel within a mouse nerve and enables doseefficient three-dimensional cellular imaging as well as two-dimensional imaging at high resolutions below 50 nm.
Coherent X-ray diffractive imaging on the single-cell-level of microbial samples
Since its first experimental demonstration in 1999, Coherent X-Ray Diffractive Imaging has become one of the most promising high resolution X-Ray imaging techniques using coherent radiation produced by brilliant synchrotron storage rings. The ability to directly invert diffraction data with the help of advanced algorithms has paved the way for microscopic investigations and wave-field analyses on the spatial scale of nanometres without the need for inefficient imaging lenses. X-Ray phase contrast which is a measure of the electron density is an important contrast mode of soft biological specimens. For the case of many dominant elements of soft biological matter, the electron density can be c...
Handbook of Lipid Membranes
This handbook provides a comprehensive overview of lipid membrane fundamentals and applications. It gives the fundamental physical and biochemical aspects of membrane-related processes in living cells, and then relates them to how scientists are building bioinspired, artifical membrane-based systems such as gene delivery vehicles and synthetic membrane interfaces. It highlights the driving mechanism behind lipid self-assembly, membrane shape evolution, and vesicle trafficing, as well as the role of lipid membrane composition in signalling and the structural aspects of membranes in cellular integrity. Key Features: Includes a broad overview on the role of lipids as structural components of me...
A Study on New Approaches in Coherent X-ray Microscopy of Biological Specimens
The use of coherent x rays for microscopic imaging has seen a rapid and ongoing development within the past decade, driven by an increasing availability of highly brilliant and coherent sources worldwide. Accordingly, novel methods have been developed, which replace the microscope‘s objective lens by a numerical reconstruction scheme. The aim of the present work is to study how very recent experimental and algorithmic developments in the field can be implemented towards a highly sensitive and fully quantitative microscopy method for imaging of biological cells. To this end, different experimental approaches are studied, based on coherent far-field as well as near-field diffraction. At firs...
