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Phage-display technology has begun to make critical contributions to the study of molecular recognition. DNA sequences are cloned into phage, which then present on their surface the proteins encoded by the DNA. Individual phage are rescued through interaction of the displayed protein with a ligand, and the specific phage is amplified by infection of bacteria. Phage-display technology is powerful but challenging and the aim of this manual is to provide comprehensive instruction in its theoretical and applied so that any scientist with even modest molecular biology experience can effectively employ it. The manual reflects nearly a decade of experience with students of greatly varying technical expertise andexperience who attended a course on the technology at Cold Spring Harbor Laboratory. Phage-display technology is growing in importance and power. This manual is an unrivalled source of expertise in its execution and application.
This book provides a comprehensive overview of the multiple strategies that plants have developed to cope with drought, one of the most severe environmental stresses. Experts in the field present 17 chapters, each of which focuses on a basic concept as well as the latest findings. The following major aspects are covered in the book: · Morphological and anatomical adaptations · Physiological responses · Biochemical and molecular responses · Ecophysiological responses · Responses to drought under field conditions The contributions will serve as an invaluable source of information for researchers and advanced students in the fields of plant sciences, agriculture, ecophysiology, biochemistry and molecular biology.
Understanding the cybersecurity threat landscape is critical to mitigating threats, apportioning limited resources, and hosting a resilient, safe, and secure Olympic Games. To support the security goals of Tokyo 2020, this report characterizes the cybersecurity threats that are likely to pose a risk to the games, visualizes a threat actor typology, and presents a series of policy options to guide cybersecurity planning.
In Antibody Phage Display expert researchers explore the latest in this cutting-edge technology, providing an invaluable resource that will guide readers in the design and execution of experiments based around antibody phage display.
Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. "Antibody Engineering" provides a comprehensive toolbox covering the well-established basics but also many exciting new techniques. The protocols reflect the latest "hands on" knowledge of key laboratories in this still fast-moving field. Newcomers will benefit from the proven step-by-step protocols, which include helpful practical advice; experienced antibody engineers will appreciate the new ideas and approaches. The book is an invaluable resource for all those engaged in antibody research and development.
With the growing global fear of a major pandemic, avian influenza (AI) virus research has greatly increased in importance. In Avian Influenza Virus, an expert team of researchers and diagnosticians examine the fundamental, yet essential, virological methods for AI virus research and diagnostics as well as some of the newest molecular procedures currently used for basic and applied research. They present exciting, cutting-edge new methods that focus both on studying the virus itself and on work with avian hosts, an area greatly lacking in research.
Today, arylation methods are belonging to the most important reaction types in organic synthesis. Lutz Ackermann, a young and ambitious professor has gathered a number of top international authors to present the first comprehensive book on the topic. Starting from a historical review, the book covers hot topics like Palladium-catalyzed arylation of N-H and alpha-C-H-acidic Bonds, Copper-catalyzed arylation of N-H and O-H Bonds, direct arylation reactions, carbanion aromatic synthesis, arylation reactions of alkenes, alkynes and much more. This compact source of high quality information is indispensable to synthetic chemists and those working in the pharmaceutical and chemical industry.
Elections, Parties and Representation in Post-Communist Europe 1990-2002 stresses the ways in which the development of political parties affected the quality of democracy, the nature of political representation, and political accountability in the early stages of post-communist politics. It also analyzes the nature and consequences of the corpus of parliamentary candidates and deputies for the representation of social classes, women and minorities. In contrast with the wide social profile of communist parliaments, politics largely became the playground of new highly educated male elites.
This book provides a comprehensive understanding of each aspect of offshore operations including conventional methods of operations, emerging technologies, legislations, health, safety and environment impact of offshore operations. The book starts by coverage of notable offshore fields across the globe and the statistics of present oil production, covering all types of platforms available along with their structural details. Further, it discusses production, storage and transportation, production equipment, safety systems, automation, storage facilities and transportation. Book ends with common legislation acts and comparison of different legislation acts of major oil/gas producing nations. ...