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Electroporation and Electrofusion in Cell Biology
Cells can be funny. Try to grow them with a slightly wrong recipe, and they turn over and die. But hit them with an electric field strong enough to knock over a horse, and they do enough things to justify international meetings, to fill a sizable book, and to lead one to speak of an entirely new technology for cell manipulation. The very improbability of these events not only raises questions about why things happen but also leads to a long list of practical systems in which the application of strong electric fields might enable the merger of cell contents or the introduction of alien but vital material. Inevitably, the basic questions and the practical applications will not keep in step. The questions are intrinsically tough. It is hard enough to analyze the action of the relatively weak fields that rotate or align cells, but it is nearly impossible to predict responses to the cell-shredding bursts of electricity that cause them to fuse or to open up to very large molecular assemblies. Even so, theoretical studies and systematic examination of model systems have produced some creditable results, ideas which should ultimately provide hints of what to try next.
Guide to Electroporation and Electrofusion
Electroporation is an efficient method to introduce macromolecules such as DNA into a wide variety of cells. Electrofusion results in the fusion of cells and can be used to produce genetic hybrids or hybridoma cells.Guide to Electroporation and Electrofusion is designed to serve the needs of students, experienced researchers, and newcomers to the field. It is a comprehensive manual that presents, in one source, up-to-date, easy-to-follow protocols necessary for efficient electroporation and electrofusion of bacteria, yeast, and plant and animal cells, as well as background information to help users optimize their results through comprehension of the principles behind these techniques. - Cove...
Cell Fusion
Over the last decade the volume Membrane Fusion. edited by Poste and Nicholson, has probably served as one of the major sources of review in formation on fusion in membrane systems. Since its publication much new information has been collected. New methods of inducing fusion have been invented or discovered, and new applications for fusion have been found. The need for an up-to-date monograph that covers and in tegrates these subjects, reviews established material, and rationalizes and integrates the old and the new is thus obvious. This book is the product of efforts to meet this need. Most of the current work in the field of membrane fusion takes place within the context of intact or modified cells. Hence this book emphasizes the plasma membrane. Each chapter is either a review, a report, or a short historical overview, depending, respectively, on whether the subject is large in scope and has a long history, or the subject is in such an early stage of development that most of what is known is still in the hands of a relatively small number of investigators and is best covered in report form.
Permeability and Stability of Lipid Bilayers
This book presents a comprehensive and coherent picture of how molecules diffuse across a liquid that is, on average, only two molecules thick. It begins by characterizing bilayers structurally, using X-ray diffraction, and then mechanically by measuring elastic moduli and mechanisms of failure. Emphasis is placed on the stability and mechanical properties of plant membranes that are subject to very large osmotic and thermal stresses. Using this information, the transport of molecules of increasing complexity across bilayers is analyzed.
Molecular Mechanisms of Membrane Fusion
Membrane fusion is an important molecular event which plays a pivotal role in many dynamic cellular processes, such as exocytosis, endocytosis, membrane biogenesis, fertilization, etc. While many reports on the physico-chem1cal process involved in membrane fusion have appeared in the literature and much information has accumulated, there has been no comprehensiv~ meeting of workers in the field. A recent symposium which took place at the Center for Tomorrow, State University of New York at Buffalo, New York, April 27-29, 1987, was the first meeting of its kind to specifically address the molecular mechanisms of membrane fusion. The Symposium consisted of oral, workshop and poster presentatio...
Charge and Field Effects in Biosystems—3
We have again brought together for the Third International Symposium on Charge and Field Effects in Biosystems (July 21-27, 1991), a group of scientists whose interests reside in the fields of bioelectrochemistry, bioenergetics, and bioelectric phenomena. Like the previous symposia at the University of Nottingham (1983) and Virginia Commonwealth University (1989) the topics discussed were related to bioelectric phenomena, including solid state theoretical and experimental approaches to charge and energy transfer in biomolecular and cellular systems, ion and electron transport properties of biological and artifical membranes, the effects of electric fields on biological systems, photoinduced ...
Guide to Non-Traditional Careers in Science
Offering practical advice and stories from scientists and professionals, this guidebook aids the reader in evaluating and finding career opportunities in non-academic research fields. It demonstrates that choices are available, providing many examples of fields (for example publishing, law, public policy and business) in which people can use their scientific training to nurture a satisfying professional life. Yet it also acknowledges that there are trade-offs involved with any veer from the traditional path.
Advanced Computational and Design Techniques in Applied Electromagnetic Systems
This symposium was concerned with advanced computational and design techniques in applied electromagnetic systems including devices and materials. The scope of the proceedings cover a wide variety of topics in applied electromagnetic fields: optimal design techniques and applications, inverse problems, advanced numerical techniques, mechanism and dynamics of new actuators, physics and applications of magnetic levitation, electromagnetic propulsion and superconductivity, modeling and applications of magnetic fluid, plasma and arc discharge, high-frequency field computations, electronic device simulations and magnetic materials.
