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Synthetic Biology
This book provides a comprehensive, up-to-date overview of the opportunities and challenges of the complex field of synthetic biology, which combines various scientific disciplines. The emerging field of synthetic biology employs biotechnological approaches to recreate and enhance basic biological structures, intracellular processes and whole organisms. The book addresses a broad range of topics, including redesigning complex metabolic pathways, DNA/RNA and protein engineering, as well as novel synthetic biomaterials. It discusses both “bottom up” and “top down” approaches and presents the latest genome engineering tools with predictions about how these could change our way of thinking and working. Since the use of synthetic biology raises a number of ethical questions, a chapter is devoted to public awareness and risk management.The book is of interest to scientists from both academia and industry, as well as PhD students and postdocs working in the field
Inclusion Bodies
This detailed volume presents a series of protocols dealing with different aspects of inclusion body (IB) processing, from cloning procedures to purification of refolded product. Commencing with chapters on upstream processing, looking into different expression strategies for IB production, the book continues with downstream applications, highlighting early protein purification and subsequent analytics, as well as success stories of IB-based processes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Inclusion Bodies: Methods and Protocols serves as an ideal resource for facilitating diverse aspects of IB processing.
Handbook of Biofunctional Surfaces
The design and synthesis of molecularly or supramolecularly defined interfacial architectures have seen in recent years a remarkable growth of interest and scientific research activities for various reasons. On the one hand, it is generally believed that the construction of an interactive interface between the living world of cells, tissue, or whol
Probes and Tags to Study Biomolecular Function
The only resource to systematically review current experimental methods, this handy reference enables researchers to select the best solution for their experimental problems. For each method covered, the book provides step-by-step protocols, illustrated by typical research applications. After an initial section on probing the lipid bilayer, the text moves on to discuss probing proteins -- including membrane proteins -- and nucleic acids. The first single publication to incorporate chemical markers, fluorescent probes and genetic tags allows a well-informed comparison of different solutions for the same problem in molecular analytics.
Novel Enzyme and Whole-Cell Biocatalysts
The concept of a circular economy relies on waste reduction, valorization, and recycling. Global trends for “green” synthesis of chemicals have positioned the field of enzyme technology and biocatalysis (multi-enzymes and whole-cells) as an alternative for the synthesis of more social- and environmentally-responsible bio-based chemicals. Recent advances in synthetic biology, computational tools, and metabolic engineering have supported the discovery of new enzymes and the rational design of whole-cell biocatalysts. In this book, we highlight these current advances in the field of biocatalysis, with special emphasis on novel enzymes and whole-cell biocatalysts for applications in several industrial biotechnological applications.
The Unity of Science
A journey guided by science that explores the universe, the earth, and the story of life For Irwin Shapiro, science starts with questions. This book provides a broad and entertaining survey of major scientific discoveries that have changed our views of nature and, in turn, spawned further questions. Shapiro, an award-winning scientist and beloved teacher, separates his inquiry into three parts: looking up at the universe; looking down at the earth and its fossils; and looking in at the story of life. His framework encourages readers to view science as a detective story—to observe and question nature and natural phenomena, and to base all conclusions on scientific evidence. With his knowledgeable yet conversational approach, Shapiro offers an enjoyable way for the curious to learn about the foundations of a range of scientific topics: the motions of bodies in the cosmos, the history and structure of the earth, the evolution of organisms, and the search for extraterrestrial life and intelligence.
Sei naiv und mach' ein Experiment: Feodor Lynen
1964 wurden Feodor Lynen und Konrad Bloch mit dem Nobelpreis für Physiologie oder Medizin ausgezeichnet. Seit Feodor Lynens Todesjahr 1979 erhalten jährlich bis zu 150 Nachwuchswissenschaftler ein Feodor-Lynen-Stipendium für Forschung im Ausland von der Alexander von Humboldt-Stiftung (AvH)und seit 1982 gibt es die Feodor Lynen Lecture für die erfolgreichsten Biochemiker der Welt. Heike Will verfasste nun die erste Biographie in engem Austausch mit vielen Zeitzeugen über Feodor Lynen als Mensch - bayerisches Urgestein -, Wissenschaftler und diplomatischen Gestalter. Sie beschreibt die Evolution der Biochemie von den dreißiger bis zu den achtziger Jahren des 20. Jahrhunderts: Wie Feodor...
Probes and Tags to Study Biomolecular Function
The only resource to systematically review current experimental methods, this handy reference enables researchers to select the best solution for their experimental problems. For each method covered, the book provides step-by-step protocols, illustrated by typical research applications. After an initial section on probing the lipid bilayer, the text moves on to discuss probing proteins -- including membrane proteins -- and nucleic acids. The first single publication to incorporate chemical markers, fluorescent probes and genetic tags allows a well-informed comparison of different solutions for the same problem in molecular analytics.
Non-Natural Amino Acids
By combining the tools of organic chemistry with those of physical biochemistry and cell biology, Non-Natural Amino Acids aims to provide fundamental insights into how proteins work within the context of complex biological systems of biomedical interest. The critically acclaimed laboratory standard for 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. With more than 400 volumes published, each Methods in Enzymology volume presents material that is relevant in today's labs -- truly an essential publication for rese...
