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PCR Protocols
In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.
Essential Ion Channel Methods
The rapid growth of interest and research activity in ion channels is indicative of their fundamental importance in the maintenance of the living state. This volume was prepared with a view toward providing a sampling of the range of molecular and physical methods that are significant for the study of ion channels. As part of the Reliable Lab Solutions series, Essential Ion Channel Methods brings together chapters from volumes 293 and 294 of Methods in Enzymology. The chapters have been selected by the editor and updated, when possible, by their original authors to include new research and references. The result is a set of chapters which make use of graphics, comparisons to other methods, a...
Aquaporins
The aquaporin field has matured at an exceptionally fast pace and we are at the verge to develop serious strategies to therapeutically modulate aquaporin function directly or via regulatory networks. Key prerequisites are available today: i. a considerable (and growing) number of aquaporin crystal structures for the rational design of inhibitory molecules, ii. elaborate molecular dynamics simulation techniques for theoretical analyses of selectivity mechanisms and docking experiments, iii. comprehensive data on aquaporin immunohistochemistry, iv. aquaporin knockout animals for physiological studies, and v. assay systems for compound library screenings. The structure of this volume on aquaporins follows the points laid out above and thus covers the developments from basic research to potential pharmacological use. Situated between pharmacology textbooks and recent scientific papers this book provides a timely overview for readers from the fundamental as well as the applied disciplines.
Vitamins and Hormones
Vitamins and Hormones is the longest-running serial published by Academic Press. The Editorial Board reflects expertise in the fields of hormone action, vitamin action, X-ray crystal structure, physiology, and enzyme mechanisms. Every volume contains comprehensive reviews by leading contributors.
Opening Windows
The third decennial review from the International Association for Society and Natural Resources, Opening Windowssimultaneously examines the breadth and societal relevance of Society and Natural Resources (SNR) knowledge, explores emergent issues and new directions in SNR scholarship, and captures the increasing diversity of SNR research. Authors from various backgrounds—career stage, gender and sexuality, race/ethnicity, and global region—provide a fresh, nuanced, and critical look at the field from both researchers’ and practitioners’ perspectives. This reflexive book is organized around four key themes: diversity and justice, governance and power, engagement and elicitation, and re...
Pierre-Esprit Radisson
VOLUME 2 will contain The Port Nelson Relations, Miscellaneous Writings, and Related Documents.
Erythrocytes: Physiology And Pathophysiology
The book covers the functional significance and properties of erythrocytes, their generation, senescence, and suicidal death. It further summarizes knowledge about hormones influencing erythrocyte formation including erythropoietin as well as disorders affecting and involving erythrocytes such as anemia, malaria, and sepsis.This seminal work forms a unique reference on the most abundant cell type in mammals and will be an invaluable resource for students in the life sciences./a
Successful Adaptation to Climate Change
What does successful adaptation look like? This is a question we are frequently asked by planners, policy makers and other professionals charged with the task of developing and implementing adaptation strategies. While adaptation is increasingly recognized as an important climate risk management strategy, and on-the-ground adaptation planning activity is becoming more common-place, there is no clear guidance as to what success would look like, what to aim for and how to judge progress. This edited volume makes significant progress toward unpacking the question of successful adaptation, offering both scientifically informed and practice-relevant answers from various sectors and regions of the...
The Nucleic Acid Protocols Handbook
A comprehensive treasury of all the key molecular biology methods-ranging from DNA extraction to gene localization in situ-needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular BiologyOao series, providing an introduction to the scientific basis of each technique, a complete listing of all the necessary materials and reagents, and clear step-by-step instruction to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate methods, and explanations of the reasons for certain steps-all key elements contributing significantly to success or failure in the lab. The Nucleic Acid Protocols Handbook constitutes today's most comprehensive collection of all the key classic and cutting-edge techniques for the successful isolation, analysis, and manipulation of nucleic acids by both experienced researchers and those new to the field."
