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Molecularly Imprinted Polymers in Biotechnology
Controlled radical polymerization techniques for molecular imprinting, by Mark E. Byrne From bulk polymers to nanoparticles, by Lei Ye Post-imprinting and in-cavity functionalization, by Toshifumi Takeuchi Characterization of MIPs (affinity, selectivity, site heterogeneity...), by Richard Ansell Theoretical aspects and computer modelling, by Ian Nicholls MIPs in aqueous environments, by Bin Lu MIPs for binding macromolecules, by Kenneth J. Shea Solid phase extraction, by Ecevit Yilmaz Sensors, by Sergey A. Piletsky MIPs for catalysis and synthesis, by Marina Resmini Wastewater treatment, by Bo Mattiasson MIPs as tools for bioassays, biotransformation and drug delivery, by Meiping Zhao
Macroporous Polymers
Macroporous polymers are rapidly becoming the material of choice for many tissue engineering, bioseparation, and bioprocessing applications. However, while important information is scattered about in many different publications, none, to date, have drawn this information together in user-friendly format, until now. Meeting the need for an accessible, organized resource, Macroporous Polymers: Production Properties and Biotechnological/Biomedical Applications supplies a systematic presentation of the production, characterization, and application of these polymers. The text discusses traditional methods of production, including phase separation polymerization, leaching, foaming, and double emul...
Immobilized Cells and Organelles
Cells and organelles are small units for biochemical synthetic purposes, often the smallest practically feasible unit since they contain coenzyme regenerating system, ordered enzyme sequences, etc.These volumes, besides giving some insight into basic technology (immobilisation procedures, etc.), also sum up the current know-how in this subject area and try to predict some future trends.The termimmobilized cells covers everything from dead cells with a single active enzyme species to cells proliferating on or within a three dimensional polymer matrix. The practical handling of these structures make them useful in various applications, e.g. large-scale production of biomolecules, biodegration, analysis, etc.
Expanded Bed Chromatography
Expanded bed adsorption chromatography is a novel processing technique for the purification of biomolecules, combining clarification, concentration and initial purification in one step. By such an integration it is possible to reduce the number of steps in the purification process, to shorten the processing time and to improve the yields. The technology is new, and interesting developments have taken place concerning the adsorbents, the processing technology and potential applications. Both small-scale laboratory processes and larger industrial processes are being developed. Expanded bed chromatography is one of the most exciting new developments in downstream processing in recent years. The technology will be a standard procedure when new biotechnological processes are being developed.
Biotechnology
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Smart Polymers for Bioseparation and Bioprocessing
Smart polymers are macromolecules capable of undergoing rapid, reversible phase transitions from a hydrophilic to a hydrophobic microstructure when triggered by small changes in their immediate environment, such as slight variations in temperature, pH or ionic strength. Until now, it has always been considered that polymers are passive participants within the Bioseparation procedure. Smart Polymers for Bioseparation and Bioprocessing addresses an entirely novel theory that advocates a much more active role for smart polymers within this process than has previously been envisaged, and therefore focuses on the role of these smart polymers within bioseparation. With contributions from the leading researchers working on smart polymers and their applications, this volume offers a comprehensive overview of both the current state-of-affairs within this research field and the potential for future developments. This book will be of interest to those working on techniques of bioseparation and bioprocessing, polymer chemists developing new smart polymers, as well as graduates in biotechnology.
Aqueous Two-Phase Systems
General methodology and apparatus: phase diagrams, preparation and analysis of two-phase systems, partioning and affinity partitioning of macromolecules: Proteins, nucleic acids, studies on protein interactionsmolecular structure, charge, hydrophobicity, and conformational chan ges, partitioning and affinity partitioning of particulates,organellesseparation and subfractionation, menbrane: separation and subfractionation, membrane domain analysis, aqueous phase separation in biologicalsystems, aqueous two-phase systems in large-scale process biotechnolo gy, proteins; downstream processing, design of proteins for enhanced extraction, other applications of aqueous phases in biotechnology. Enzymology.
Smart Polymers
The first book to tackle the application of smart polymers in bioseparation and bioprocessing, Smart Polymers: Applications in Biotechnology and Biomedicine broke new ground in this challenging field. Completely revised, updated, and following in the footsteps of its predecessor, the second edition is poised to take its place as a premier reference
TRAC: Trends in Analytical Chemistry
Trends in Analytical Chemistry, Volume 3 focuses on developments in analytical chemistry, including the adoption of automation in laboratory processes, chromatography, and flow analysis. The selection first underscores the effect of automation on the operations of analytical laboratories and techniques for the automated optimization of HPLC separations. Topics include initial requirements, window diagrams, and chemometric approaches. The text then ponders on generation of statistical tables by microcomputer; enzyme electrodes for continuous in-vivo monitoring; and enantiomeric analysis of the common protein amino acids by liquid chromatography. The publication takes a look at sample preparation for the analysis of heavy metals in foods and application of ion-selective electrodes in flow analysis, including dry ashing, acid extraction, and ion-selective electrodes in flowing systems. The text then examines trends in laboratory information management systems; zone electrophoresis in open-tubular capillaries; and using computers to interpret IR spectra of complex molecules. The selection is a valuable source of data for readers interested in the developments in analytical chemistry.
Molecular Interactions in Bioseparations
Rapid advances in molecular biology have accelerated the production of a great number of protein-based therapeutic agents. The major cost in producing these proteins appears to be associated with their purification from the complex mixture of the crude extract. A major challenge to the protein biochemist and the biochemical engineer is the development of rapid, efficient, and cost-effective purification systems. This volume presents state-of-the-art reviews of current methods used in the purifica tion of biological macromolecules that are based on molecular interactions. Thus, the major emphasis is placed on affinity-related techniques. Part I provides a general introduction to affinity chro...
