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In Protein Stability and Folding: Theory and Practice, world-class scientists present in a single volume a comprehensive selection of hands-on recipes for all of the major techniques needed to understand the conformational stability of proteins, as well as their three-dimensional folding. The distinguished contributors provide clear, step-by-step instructions along with many troubleshooting tips, alternative procedures, and informative explanations about why certain steps are necessary. Even highly skilled researchers will find many time-saving methods. Among the techniques discussed are fluorescent, ultraviolet, and infrared spectroscopy; HPLC peptide mapping; differential scanning calorimetry; and hydrogen exchange. Shirley's Protein Stability and Folding: Theory and Practice will ensure a significant difference in the outcome of your experiments, producing the result desired even for beginners.
This book examines the role that the American Film Institute (AFI) had in supporting experimental and independent cinema at a key moment of change in the history of American film. Weaving a rich historical narrative, Ramirez argues that the Cold War struggle for cultural supremacy motivated the creation of the federally-funded AFI. Exploring the intersection of business interests and political objectives, Ramirez demonstrates how the AFI’s approach to experimental and independent cinema was marked by an interest in promoting innovative aesthetics and protecting the creative freedom of filmmakers but lacked the attention to distribution and exhibition that would strengthen the viability of experimental and independent filmmaking as professional practices. Scholars of film, history, and American studies will find this work particularly useful.
The Routledge Companion to Ecopoetics offers comprehensive coverage of the vital and growing movement of ecopoetics. This volume begins with a general introduction to the field, followed by six sections: Perspectives: broad overviews engaging fields such as biosemiosis, kinship praxis, and philosophical approaches Experiments: formal innovations developed by poets in response to planetary crises Earth and Water: explorations of poetic entanglement with planetary chemical and biological systems Waste/Toxicity/Precarity: poetics addressing the effects of pollution and climate change Environmental Justice and Activism: examinations of poetry as an engine of political and cultural change Region ...
'A wonderfully mysterious book . . . unforgettably rich' ANNE TYLER Caro, gallant and adventurous, is one of two Australian sisters who have come to post-war England to seek their fortunes. Courted long and hopelessly by young scientist, Ted Tice, she is to find that love brings passion, sorrow, betrayal and finally hope. The milder Grace seeks fulfilment in an apparently happy marriage. But as the decades pass and the characters weave in and out of each other's lives, love, death and two slow-burning secrets wait in ambush for them. From the Orange Prize shortlisted author of THE GREAT FIRE
OVERCOMING CELEBRITY OBSESSIONis a star-studded journey from celebrity obsession to fulfilling life in three easy phases. When a fan is obsessed, a story character version of the celebrity is created. It is through the fans celebrity characterization that guides the fan through Phase 1. Upon understanding why the fan was obsessed in the first place, the journey through Phase 2 begins. This is the dark part of the fans life that the fan used the favorite celebrity to escape from. Professional counseling is not recommended in Phase 1, but can begin in Phase 2. In order to get the most from Phase 3, the fan must be able to look at parts of his or her real life and pull out the celebrity obsessi...
Since the dawn of recorded history, and probably even before, men and women have been grasping at the mechanisms by which they themselves exist. Only relatively recently, did this grasp yield anything of substance, and only within the last several decades did the proteins play a pivotal role in this existence. In this expose on the topic of protein structure some of the current issues in this scientific field are discussed. The aim is that a non-expert can gain some appreciation for the intricacies involved, and in the current state of affairs. The expert meanwhile, we hope, can gain a deeper understanding of the topic.
Twenty-three letters written by the author to her sister, Mary Jane, in Massachusetts, under the pseud., Dame Shirley.
THE CONTROVERSIAL SUNDAY TIMES BESTSELLER. Candid, fearless and provocative – the author of American Psycho on who he is and what he thinks is wrong with the world today. Bret Easton Ellis is most famous for his era-defining novel American Psycho and its terrifying anti-hero, Patrick Bateman. With that book, and many times since, Ellis proved himself to be one of the world’s most fearless and clear-sighted observers of society – the glittering surface and the darkness beneath. In White, his first work of non-fiction, Ellis offers a wide-ranging exploration of what the hell is going on right now. He tells personal stories from his own life. He writes with razor-sharp precision about the...