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Controlling Osteoblast Morphology and Proliferation Via Surface Micro-topographies of Implant Biomaterials
Abstract: Current research on surface modifications has yielded advanced implant biomaterials. Various implant surface modifications have been shown to be promising in improving bone target cell response, but more comprehensive studies whether certain implant surface modifications can directly target cell behavioural features such as morphogenesis and proliferation are needed. Here, we studied the response of primary alveolar bone cells on various implant surface modifications in terms of osteoblast morphology and proliferation in vitro. Analyses of surface modifications led to surface-related test parameters including the topographical parameters micro-roughness, texture aspect and surface ...
Effect of Hydrogen Peroxide on the Surface and Attractiveness of Various Zirconia Implant Materials on Human Osteoblasts: an in Vitro Study
Abstract: The aim of this in vitro study was to investigate the effect of hydrogen peroxide (H2O2) on the surface properties of various zirconia-based dental implant materials and the response of human alveolar bone osteoblasts. For this purpose, discs of two zirconia-based materials with smooth and roughened surfaces were immersed in 20% H2O2 for two hours. Scanning electron and atomic force microscopy showed no topographic changes after H2O2-treatment. Contact angle measurements (1), X-ray photoelectron spectroscopy (2) and X-ray diffraction (3) indicated that H2O2-treated surfaces (1) increased in hydrophilicity (p
Bioimplants Manufacturing
The text covers fundamentals and technological advancements in processing, post-processing, and surface engineering of bioimplant materials. It further discusses important topics such as the additive manufacturing of bioimplants, the tribological performance of bioimplants, and the hybrid and non-traditional manufacturing of bioimplants materials. The text also presents the latest advancements in intelligent bioimplant manufacturing using artificial intelligence and machine learning. This book: Offers an in-depth understanding of the fundamentals, types, materials and applications of bioimplants Highlights the effect of processing on microstructure, biocompatibility, and mechanical behavior ...
Advanced 3D Cell Culture Techniques in Micro-bioreactors, Part II: Systems and Applications
Abstract: In this second part of our systematic review on the research area of 3D cell culture in micro-bioreactors we give a detailed description of the published work with regard to the existing micro-bioreactor types and their applications, and highlight important results gathered with the respective systems. As an interesting detail, we found that micro-bioreactors have already been used in SARS-CoV research prior to the SARS-CoV2 pandemic. As our literature research revealed a variety of 3D cell culture configurations in the examined bioreactor systems, we defined in review part one "complexity levels" by means of the corresponding 3D cell culture techniques applied in the systems. The definition of the complexity is thereby based on the knowledge that the spatial distribution of cell-extracellular matrix interactions and the spatial distribution of homologous and heterologous cell-cell contacts play an important role in modulating cell functions. Because at least one of these parameters can be assigned to the 3D cell culture techniques discussed in the present review, we structured the studies according to the complexity levels applied in the MBR systems
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Identification of Zirconia Particle Uptake in Human Osteoblasts by ToF-SIMS Analysis and Particle-size Effects on Cell Metabolism
Abstract: As the use of zirconia-based nano-ceramics is rising in dentistry, the examination of possible biological effects caused by released nanoparticles on oral target tissues, such as bone, is gaining importance. The aim of this investigation was to identify a possible internalization of differently sized zirconia nanoparticles (ZrNP) into human osteoblasts applying Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS), and to examine whether ZrNP exposure affected the metabolic activity of the cells. Since ToF-SIMS has a low probing depth (about 5 nm), visualizing the ZrNP required the controlled erosion of the sample by oxygen bombardment. This procedure removed organic matter, un...
Methods in Bioengineering
Tissue engineering is an emerging field that involves the combination of materials, cells, and other signals or growth factors to generate new tissue that can be used to repair or replace damaged tissues due to injury or disease. This groundbreaking volume presents the latest methods and protocols for systematically building tissues in 3D configuration outside the body, as well as providing techniques that modulate repair and regeneration processes that occur "in situ" (in their natural or original place).
Advanced 3D Cell Culture Techniques in Micro-bioreactors. Part I: a Systematic Analysis of the Literature Published Between 2000 and 2020
Abstract: Bioreactors have proven useful for a vast amount of applications. Besides classical large-scale bioreactors and fermenters for prokaryotic and eukaryotic organisms, micro-bioreactors, as specialized bioreactor systems, have become an invaluable tool for mammalian 3D cell cultures. In this systematic review we analyze the literature in the field of eukaryotic 3D cell culture in micro-bioreactors within the last 20 years. For this, we define complexity levels with regard to the cellular 3D microenvironment concerning cell-matrix-contact, cell-cell-contact and the number of different cell types present at the same time. Moreover, we examine the data with regard to the micro-bioreactor design including mode of cell stimulation/nutrient supply and materials used for the micro-bioreactors, the corresponding 3D cell culture techniques and the related cellular microenvironment, the cell types and in vitro models used. As a data source we used the National Library of Medicine and analyzed the studies published from 2000 to 2020
Benjamin and Vladka Meed Registry of Jewish Holocaust Survivors 2000
"This book is based on the records of the Benjamin and Vladka Meed Registry of Jewish Holocaust Survivors. The Registry is a computer database that lists more than 170,000 names of Holocaust survivors and some members of their families. The American Gathering of Jewish Holocaust Survivors first established a national registry in 1981 to document the lives of survivors who came to the United States after World War II ... The Registry includes the names of Holocaust survivors who are now deceased, but does not indicate that they have passed away ... this published version only includes information about the survivors based on their individual files."--Introduction
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