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Over the past century, studies of the budding yeast Saccharomyces cerevisiae have helped to unravel principles of nearly every aspect of eukaryotic cell biologyfrom metabolism and molecular genetics to cell division and differentiation. Thanks to its short generation time, ease of genetic manipulation, and suitability for high-throughput studies, yeast remains the focus of research in a vast number of laboratories worldwide. This laboratory manual provides a comprehensive collection of experimental procedures that continue to make budding yeast an informative model. The contributors describe methods for culturing and genetically modifying yeast, strategies and tools (e.g., gene deletion coll...
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George Boone IV (1690-1753), a Quaker, emigrated from England to Abington, Philadelphia County, Pennsylvania, married Deborah Howell in 1713, and moved to Berks County, Pennsylvania. Descendants lived in Pennsylvania, Ohio, Kentucky, Tennessee, Texas, New Mexico, Colorado, California and elsewhere.
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