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Muscle Stem Cells
This volume provides leading-edge protocols in the study of the molecular and cellular biology of muscle stem cells. Chapters detail current and updated methods for muscle stem cell isolation, culture, molecular analysis, cellular analysis, and reintroduction in vivo as well as protocols for studying myogenic stem cells in non-mammalian model systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Muscle Stem Cells: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Stem Cells and Cell Signalling in Skeletal Myogenesis
Skeletal muscle development is perhaps one of the best understood processes at the molecular, cellular and organismal level due in large part to the fact that primary myogenic cells (myoblasts) will grow and subsequently differentiate into myotubes in culture. With the advent of reverse mouse genetics, many of the observations gained through the study of myogenic cells in vitro have been directly tested in vivo. What has emerged is a complex but cohesive story of how myogenic cells are initially specified in the vertebrate embryo and how muscle fibers ultimately achieve their respective identities (i.e. fast versus slow) to perform their function. This collection of chapters is focused on these developments. The book discusses old and new directions for the skeletal muscle field and points out directions where the field may eventually progress.
Tissue Restoration Through Regenerative Biology and Medicine
Advances in cell, developmental and molecular biology, and the discovery of regeneration-competent cells in many non-regenerating mammalian tissues, have given impetus to systematic investigations that will enable us to regenerate these tissues by cell transplantation or the pharmaceutical induction of regeneration from the body’s own tissues. A significant avenue of research is the identification of the soluble and insoluble signals and their transduction pathways that govern the proliferation and differentiation of regeneration-competent cells, and the signals that inhibit their activity after injury. How far can we go in our quest for regeneration? We will probably be able to induce the regeneration of some tissues, such as skin or even spinal cord, within a few years. The regeneration of others, such as heart, lung, kidney or appendages, may be more complex and difficult, but we should not view them as impossible. They will just take a little longer.
Muscle Cell and Tissue
In order to complete tissue regeneration, various cells such as neuronal, skeletal, smooth, endothelial, and immune (e.g., macrophage) interact smoothly with each other. This book, Muscle Cells and Tissues, offers a wide range of topics such as stem cells, cell culture, biomaterials, epigenetics, therapeutics, and the creation of tissues and organs. Novel applications for cell and tissue engineering including cell therapy, tissue models, and disease pathology modeling are discussed. The book also deals with the functional role of autophagy in modulating muscle homeostasis and molecular mechanism regulating skeletal muscle mass. The chapters can be interesting for graduate students, postdocs, teachers, physicians, and for executives in biotech and pharmaceutical companies, as well as researchers in the fields of molecular biology and regenerative medicine.
Stem Cells
This third in the Current Topics in Molecular Cell Biology and Molecular Medicine Series contains a careful selection of new and updated, high-quality articles from the well-known Meyer's Encyclopedia, describing new perspectives in stem cell research. The 26 chapters are divided into four sections: Basic Biology, Stem Cells and Disease, Stem Cell Therapy Approaches, and Laboratory Methods, with the authors chosen from among the leaders in their respective fields. This volume represents an essential guide for students and researchers seeking an overview of the field.
Forensic DNA Applications
Forensic DNA Applications: An Interdisciplinary Perspective was developed as an outgrowth of a conference held by the International Society of Applied Biological Sciences. The topic was human genome–based applications in forensic science, anthropology, and individualized medicine. Assembling the contributions of contributors from numerous regions around the world, this volume is designed as both a textbook for forensic molecular biology students and a reference for practitioners and those in the legal system. The book begins with the history and development of DNA typing and profiling for criminal and civil purposes. It discusses the statistical interpretation of results with case examples...
Craniofacial Muscles
Of the approximately 640 muscles in the human body, over 10% of them are found in the craniofacial region. The craniofacial muscles are involved in a number of crucial non-locomotor activities, and are critical to the most basic functions of life, including vision, taste, chewing and food manipulation, swallowing, respiration, speech, as well as regulating facial expression and controlling facial aperture patency. Despite their importance, the biology of these small skeletal muscles is relatively unexplored. Only recently have we begun to understand their unique embryonic development and the genes that control it and characteristic features that separate them from the skeletal muscle stereot...
New Perspectives in Regeneration
Regeneration, the homeostatic ability to maintain tissue structure in the face of normal cell turnover or loss of tissue damaged by trauma or disease, is an essential developmental process that continues throughout life. As recently as a decade ago, any serious discussion of the possibility of regeneration becoming a practical medical tool in the near future had the air of science fiction or over-optimistic speculation. The term “regenerative medicine” was certainly on many lips but few actually expected to soon see it applied in a clinical setting. A tidal wave of discovery has changed that and investigating the cellular mechanisms of natural regeneration has become one of the hottest t...
Muscle Homeostasis and Regeneration
The book is a collection of original research and review articles addressing the intriguing field of the cellular and molecular players involved in muscle homeostasis and regeneration. One of the most ambitious aspirations of modern medical science is the possibility of regenerating any damaged part of the body, including skeletal muscle. This desire has prompted clinicians and researchers to search for innovative technologies aimed at replacing organs and tissues that are compromised. In this context, the papers, collected in this book, addressing a specific aspects of muscle homeostasis and regeneration under physiopathologic conditions, will help us to better understand the underlying mechanisms of muscle healing and will help to design more appropriate therapeutic approaches to improve muscle regeneration and to counteract muscle diseases.
Muscle Stem Cells
Muscle Stem Cells, Volume 158 in the Current Topics of Developmental Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics surrounding Muscle stem cell dysfunction in rhabdomyosarcoma and muscular dystrophy, Model systems used to study MuSC function, MuSCs in the growth and maintenance of muscle, Molecular regulation of myocyte fusion, A self-made quiescent niche of muscle stem cells, Characterization of the muscle regenerative environment, Role of microenvironment on muscle stem cell function in health, adaptation, and disease, Vascular Niche for Muscle Stem Cells, Regulation of muscle stem cell polarity in health and disease, a...
