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Intraspecific differences in erythrocyte antigens (blood types) were shown to occur in four species of Pacific salmon, the sockeye or red salmon (Oncorhynchus nerka), the chinook or king salmon (O. tshawytscha), the chum salmon (O. keta), and the pink salmon (O. gorbuscha). Antisalmon-erythrocyte sera prepared in rabbits and chickens were used after absorption of species-specific antibodies. Some of these blood types were shown to differ in their frequency of occurrence between different geographic races. In addition, isoimmunizations were prepared and at least eight different patterns of antigenic composition were displayed by the cells tested. These results indicate that considerable antigenic diversity exists in salmon. Reagents to detect valuable markers for the investigation of geographic races of salmon should be obtained through further research.
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Methods of Animal Experimentation, Volume III, compiles methods of animal experimentation in a variety of fields so that the researcher might have a reference that would allow him to incorporate other techniques in his research studies. The methods described represent only the most efficient ways known to date for using animals to gain research information. They are not, however, ""standard methods"" for research in which each experiment seeks or confirms new information by another means. The largest portion of this volume is devoted to behavioral science because of the growing importance of this field in two respects. First, it is an important consideration in all experiments in which anima...