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This is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years.
Since its introduction in the late 1980s, DNA analysis has revolutionized the forensic sciences: it has helped to convict the guilty, exonerate the wrongfully convicted, identify victims of mass atrocities, and reunite families whose members have been separated by war and repressive regimes. Yet, many of the scientific, legal, societal, and ethical concepts that underpin forensic DNA analysis remain poorly understood, and their application often controversial. Told by over twenty experts in genetics, law, and social science, Silent Witness relates the history and development of modern DNA forensics and its application in both the courtroom and humanitarian settings. Across three thematic sec...
PCR, developed at Cetus Corporation/USA by Henry A. Erlich, Kary Mullis and Randall K. Saiki, is a very simple method for amplifying nucleic acids in vitro. The realization of this idea bases on the repetition of a set of three different temperatures and yields an increase of the target structure up to a factor of 106 to 1012. Therefore, this technique is predisposed for safe analysis and characterization of DNA and RNA sequences of interest, even where the starting amount of material is enormously small. Because of its sensitivity, speed and versatility this method is particularly suitable for investigations of oncogenes, tumor associated translocations, retroviral sequences, lymphokines an...
Forensic DNA Applications: An Interdisciplinary Perspective was developed as an outgrowth of a conference held by the International Society of Applied Biological Sciences. The topic was human genome–based applications in forensic science, anthropology, and individualized medicine. Assembling the contributions of contributors from numerous regions around the world, this volume is designed as both a textbook for forensic molecular biology students and a reference for practitioners and those in the legal system. The book begins with the history and development of DNA typing and profiling for criminal and civil purposes. It discusses the statistical interpretation of results with case examples...
Polymerase chain reaction (PCR) technology is a revolutionary innovation which enables scientists to rapidly generate large amounts of genetic material from a slight trace which would otherwise be too small to analyze. With applications in both research and diagnostics, PCR is becoming a standard procedure in biotechnology and medical diagnostic laboratories. This book is an introduction and guide to the new technology, covering the basic methodologies and their applications in research and medicine, emphasizing practical aspects. Each chapter is written by pioneers in the field and most include detailed protocols and favorite PCR "recipes". Students and researchers in all areas of biotechnology and molecular biology will find this book the introduction to PCR they've been looking for.
Now that the political rhetoric can end, Erlich (Russian literature, Yale U.) examines the impact of the 1917 revolution on Russian poetry, criticism, and artistic prose. He looks at the flirtations with modernism of the early 20th century and compares the futurists, formalists, novelists, and short-story writers of the first decade of the new social and political order. Assumes no knowledge of Russian. Annotation copyright by Book News, Inc., Portland, OR
This book presents key methodologies, tools and databases for biochemistry, microbiology and molecular biology in simple and straightforward language. Covering all aspects related to experimental principles and procedures, the protocols included here are brief and clearly defined, and include essential precautions to be taken while conducting experiments. The book is divided into two major sections: one on constructing, working with, and standard operating procedures for laboratory instruments; and one on practical procedures used in molecular biology, microbiology and biochemical analysis experiments, which are described in full. Each chapter describes both the basic theory and relevant pra...
A perceptive literary critic, a world-famous writer of witty and playful verses for children, a leading authority on children’s linguistic creativity, and a highly skilled translator, Kornei Chukovsky was a complete man of letters. As benefactor to many writers including Alexander Solzhenitsyn and Joseph Brodsky, he stood for several decades at the center of the Russian literary milieu. It is no exaggeration to claim that Chukovsky knew everyone involved in shaping the course of twentieth-century Russian literature. His voluminous diary, here translated into English for the first time, begins in prerevolutionary Russia and spans nearly the entire Soviet era. It is the candid commentary of ...
This book represents a new and hopeful paradigm for treatment of diseases that are spreading globally as countries adopt Western lifestyles and standards of living. It describes the phenomenal science and clinical efficacy of the work of Dr Xiu-Min Li across a broad array of immune and inflammatory diseases. These include food allergies, asthma, eczema, 'new' diseases such as mast cell disorders, obesity, and mental health problems that are part of a worldwide 'epidemic of progress'.The most allergic people are caught in a cycle of medication, steroid dependency, emergency hospitalization, and curtailing their activities and diets to avoid triggers. Children are 'losing their childhoods.' Th...