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PCR Technology
This is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years.
PCR Topics
PCR, developed at Cetus Corporation/USA by Henry A. Erlich, Kary Mullis and Randall K. Saiki, is a very simple method for amplifying nucleic acids in vitro. The realization of this idea bases on the repetition of a set of three different temperatures and yields an increase of the target structure up to a factor of 106 to 1012. Therefore, this technique is predisposed for safe analysis and characterization of DNA and RNA sequences of interest, even where the starting amount of material is enormously small. Because of its sensitivity, speed and versatility this method is particularly suitable for investigations of oncogenes, tumor associated translocations, retroviral sequences, lymphokines an...
PCR Technology
Polymerase chain reaction (PCR) technology is a revolutionary innovation which enables scientists to rapidly generate large amounts of genetic material from a slight trace which would otherwise be too small to analyze. With applications in both research and diagnostics, PCR is becoming a standard procedure in biotechnology and medical diagnostic laboratories. This book is an introduction and guide to the new technology, covering the basic methodologies and their applications in research and medicine, emphasizing practical aspects. Each chapter is written by pioneers in the field and most include detailed protocols and favorite PCR "recipes". Students and researchers in all areas of biotechnology and molecular biology will find this book the introduction to PCR they've been looking for.
Forensic DNA Applications
Forensic DNA Applications: An Interdisciplinary Perspective was developed as an outgrowth of a conference held by the International Society of Applied Biological Sciences. The topic was human genome–based applications in forensic science, anthropology, and individualized medicine. Assembling the contributions of contributors from numerous regions around the world, this volume is designed as both a textbook for forensic molecular biology students and a reference for practitioners and those in the legal system. The book begins with the history and development of DNA typing and profiling for criminal and civil purposes. It discusses the statistical interpretation of results with case examples...
Modernism and Revolution
Now that the political rhetoric can end, Erlich (Russian literature, Yale U.) examines the impact of the 1917 revolution on Russian poetry, criticism, and artistic prose. He looks at the flirtations with modernism of the early 20th century and compares the futurists, formalists, novelists, and short-story writers of the first decade of the new social and political order. Assumes no knowledge of Russian. Annotation copyright by Book News, Inc., Portland, OR
Basic Techniques in Biochemistry, Microbiology and Molecular Biology
This book presents key methodologies, tools and databases for biochemistry, microbiology and molecular biology in simple and straightforward language. Covering all aspects related to experimental principles and procedures, the protocols included here are brief and clearly defined, and include essential precautions to be taken while conducting experiments. The book is divided into two major sections: one on constructing, working with, and standard operating procedures for laboratory instruments; and one on practical procedures used in molecular biology, microbiology and biochemical analysis experiments, which are described in full. Each chapter describes both the basic theory and relevant pra...
Making PCR
Making PCR is the fascinating, behind-the-scenes account of the invention of one of the most significant biotech discoveries in our time—the polymerase chain reaction. Transforming the practice and potential of molecular biology, PCR extends scientists' ability to identify and manipulate genetic materials and accurately reproduces millions of copies of a given segment in a short period of time. It makes abundant what was once scarce—the genetic material required for experimentation. Making PCR explores the culture of biotechnology as it emerged at Certus Corporation during the 1980s and focuses on its distinctive configuration of scientific, technical, social, economic, political, and le...
Diary, 1901-1969
A perceptive literary critic, a world-famous writer of witty and playful verses for children, a leading authority on children’s linguistic creativity, and a highly skilled translator, Kornei Chukovsky was a complete man of letters. As benefactor to many writers including Alexander Solzhenitsyn and Joseph Brodsky, he stood for several decades at the center of the Russian literary milieu. It is no exaggeration to claim that Chukovsky knew everyone involved in shaping the course of twentieth-century Russian literature. His voluminous diary, here translated into English for the first time, begins in prerevolutionary Russia and spans nearly the entire Soviet era. It is the candid commentary of ...
Food Allergies
Food Allergies: Traditional Chinese Medicine, Western Science, and the Search for a Cure is the first book-length account of the work of Dr. Xiu-Min Li on her work to adapt ancient herbal medicines to cure "modern" diseases, and to prove their efficacy using the best that science has to offer. Born in China, Dr. Li was simultaneously trained in traditional Chinese medicine (TCM) and Western medicine in Henan and Beijing, followed by fellowships at Stanford and Johns Hopkins. Author Henry Ehrlich calls her a Rosetta stone because she can think simultaneously in both systems and recognize the potential of thousands of TCM remedies for treating diseases of the immune system that defy conventional remedies, not just food allergies but also eczema and asthma, and, potentially, autoimmune disorders. Now a full professor at Mt. Sinai in New York, her research has produced a bonanza of insight into the functioning of the immune system.--Publisher's description.
