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Laboratory Procedures and Their Applications
Automation in plant tissue culture; General introduction and overview; Economic analysis of automated micropropagation; Economic aspects of somati embryogenesis; Systems analysis and engineering; Engineering aspects of plant propagation in bioreactors; Mechanical engineering approaches to plant biotechnology; Image analysis for plant cell culture and micropropagation; Image analysis for embryogenesis; Automation of the bioreactor process for mass propagation and secondary metabolism; Delivery system for tissue culture by encapsulation; A delivery system for naked somatic embryos for interior spruce; Automated systems for organogenesis; Commercialisation of tissue culture and automated systems; Environmental control in plant tissue culture; General introduction; Physical microenvironmental adn its effects; Vessels, gels, liquid media, and support systems; The chemical mciroenvironment; Carbon nutrition in vitro; Regulation and manupulation of carbon assimilation in micropropagated systems; Ethylene; In vitro acclimatization; Low temperature storage of plant tissue cultures; Environmental measurement and control systems.
This book provides comprehensive information on micropropagation of economically important forest and fruit trees, which is usually available in scattered literature. Topics cover a wide range, from tropical forest and fruit trees for paper or food supply, to Prunus species for local craft bark production.
Automation and Environmental Control in Plant Tissue Culture rigorously explores the new challenges faced by modern plant tissue culture researchers and producers worldwide: issues of cost efficiency, automation, control, and optimization of the in vitro microenvironment. This book achieves a critical balance between the economic, engineering and biological viewpoints, and presents well-balanced, unique, and clearly organized perspectives on current initiatives in the tissue culture arena. Each chapter offers guidelines leading towards an exhaustive, unprecedented level of control over in vitro growth, based on emerging technologies of robotics, machine vision, environmental sensors and regu...
The rapid progress made on somatic embryogenesis and its prospects for potential applications in improving woody plants prompted us to edit this book initially in three volumes, and now to add two more volumes. The editors were all convinced that such a treatise was needed and would be extremely useful to researchers and students. This Volume 4 has been divided into three sections and contains 23 chapters. Section A contains eleven chapters covering studies of embryo development and cell biology of white spruce, proliferative somatic embryogenesis in woody species, somatic embryo germination and desiccation tolerance in conifers, performance of conifer somatic seedlings, apoptosis during ear...
This Volume contains the papers presented by twenty-eight invited speakers at the symposium entitled, "Genetic Manipulation of Woody Plants," held at Michigan State University, East Lansing, Michigan, from June 21-25, 1987. Also included are abstracts of contributed poster papers presented during the meeting. That the molecular biology of woody plants is a rapidly expanding field is attested to by the large attendance and high level of enthusiasm generated at the conference. Leading scientists from throughout the world discussed challenging problems and presented new insights into the devel opment of in vitro culture systems, techniques for DNA analysis and manipulation, gene vector systems,...
Scale-Up and Automation in Plant Propagation reviews methods of automation and scale-up of plant propagation in vitro. It looks at the large scale clonal propagation of plants, or micropropagation, as the first major practical application of plant biotechnology. It also discusses the advantages and limitations of micropropagation and evaluates current methods of commercial micropropagation. Organized into 13 chapters, this volume begins with an overview of the benefits of scaling up and automating plant propagation before proceeding with a discussion of synthetic seeds and their use for plant propagation, along with problems and economic considerations associated with synthetic seed technolo...
The Molecular Biology of Plastids: Cell Culture and Somatic Cell Genetics of Plants, Volume 7A deals with various aspects of plastid nucleic acid and protein metabolism. This book is organized into 10 chapters. Chapter 1 provides the introduction to the molecular biology of plastids, followed by a discussion of the maps of restriction endonuclease sites on chloroplast chromosomes in Chapter 2. Chapter 3 focuses on chloroplast gene transmission, while Chapters 4 to 7 describe the apparatus for nucleic acid and protein metabolism and how some transcripts of chloroplast genes are processed. The ribosomal proteins, ribosomes, and translation in plastids are covered in Chapter 8. The last two chapters consider the organization, operation, and transport of polypeptides through the outer plastid membranes. This volume is a good reference for plant molecular biologist, genetic engineers, and researchers conducting work on the molecular biology of chloroplasts.
We are facing global issues concerning environmental pollution and shortages of food, feed, phytomass (plant biomass) and natural resources, which will become more serious in the forthcoming decades. To solve these issues, immeasurable numbers of various plants and huge amounts of phytomass are required every year for food, feed and for the improvement of amenities, the environment and our quality of life. Increased phytomass is also required as alternative raw material for producing bio-energy, biodegradable plastics and many other plant-originated industrial products. Only by using phytomass as a reproducible energy source and raw material, instead of fossil fuels and atomic power, we can ...