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A comprehensive treasury of all the key molecular biology methods-ranging from DNA extraction to gene localization in situ-needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular BiologyOao series, providing an introduction to the scientific basis of each technique, a complete listing of all the necessary materials and reagents, and clear step-by-step instruction to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate methods, and explanations of the reasons for certain steps-all key elements contributing significantly to success or failure in the lab. The Nucleic Acid Protocols Handbook constitutes today's most comprehensive collection of all the key classic and cutting-edge techniques for the successful isolation, analysis, and manipulation of nucleic acids by both experienced researchers and those new to the field."
The two volume set LNAI 3801 and LNAI 3802 constitute the refereed proceedings of the annual International Conference on Computational Intelligence and Security, CIS 2005, held in Xi'an, China, in December 2005. The 338 revised papers presented - 254 regular and 84 extended papers - were carefully reviewed and selected from over 1800 submissions. The first volume is organized in topical sections on learning and fuzzy systems, evolutionary computation, intelligent agents and systems, intelligent information retrieval, support vector machines, swarm intelligence, data mining, pattern recognition, and applications. The second volume is subdivided in topical sections on cryptography and coding, cryptographic protocols, intrusion detection, security models and architecture, security management, watermarking and information hiding, web and network applications, image and signal processing, and applications.
It is my pleasure to write the preface for Information Processing and Management. This book aims to bring together innovative results and new research trends in inf- mation processing, computer science and management engineering. If an information processing system is able to perform useful actions for an obj- tive in a given domain, it is because the system knows something about that domain. The more knowledge it has, the more useful it can be to its users. Without that kno- edge, the system itself is useless. In the information systems field, there is conceptual modeling for the activity that elicits and describes the general knowledge a particular information system needs to know. The main objective of conceptual modeling is to obtain that description, which is called a conceptual schema. Conceptual schemas are written in languages called conceptual modeling languages. Conceptual modeling is an important part of requi- ments engineering, the first and most important phase in the development of an inf- mation system.
Sea area reclamation is one important activity where land is obtained in coastal countries and regions. It is also a marine development activity with great environment impact. Management technologies for sea area reclamation is the technologies system for sea area reclamation management.This compendium is a summary of the research and application of reclamation management technology in China. It makes a thorough study and systematic exposition of the main technical links of reclamation management.This comprehensive reference text provides a window for domestic and abroad reclamation managers, reclamation demonstration and assessment workers, reclamation project owners and even teachers and students keen in reclamation management.
Drawing mainly on advertisements and comics in Chinese newspapers, Singaporean scholar and educator Yeo Mang Thong demonstrates how Singapore was an important hub for artists who travelled to and lived in Singapore. Yeo’s research features amongst other things essays on sojourning artists, and fills a gap in scholarship on the pre-war visual arts scene in Singapore. Originally in Chinese, this English translation aims to bring his research to a broader audience.
In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.
The four-volume set LNCS 3480-3483 constitutes the refereed proceedings of the International Conference on Computational Science and Its Applications, ICCSA 2005, held in Singapore in May 2005. The four volumes present a total of 540 papers selected from around 2700 submissions. The papers span the whole range of computational science, comprising advanced applications in virtually all sciences making use of computational techniques as well as foundations, techniques, and methodologies from computer science and mathematics, such as high performance computing and communication, networking, optimization, information systems and technologies, scientific visualization, graphics, image processing, data analysis, simulation and modelling, software systems, algorithms, security, multimedia etc.
The four volume set assembled following The 2005 International Conference on Computational Science and its Applications, ICCSA 2005, held in Suntec International Convention and Exhibition Centre, Singapore, from 9 May 2005 till 12 May 2005, represents the ?ne collection of 540 refereed papers selected from nearly 2,700 submissions. Computational Science has ?rmly established itself as a vital part of many scienti?c investigations, a?ecting researchers and practitioners in areas ranging from applications such as aerospace and automotive, to emerging technologies such as bioinformatics and nanotechnologies, to core disciplines such as ma- ematics, physics, and chemistry. Due to the shear size ...