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Difference Gel Electrophoresis
The second edition of this volume provides a comprehensive update of this key method on gel-based proteomics. Chapters present an introduction into the development of methods on principles of differential protein labeling and two-dimensional gel electrophoresis, techniques on optimized proteomic workflows using advanced mass spectrometry for protein identification, and the application of those methods in basic biological research, pathobiology and applied biomarker discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Difference Gel Electrophoresis: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
Proceedings of 4th World Congress on Mass Spectrometry 2017
June 19-21, 2017 London, UK Key Topics : Applications of Mass Spectrometry, New Approaches in Mass Spectrometry, Recent Advances and Development in Mass Spectrometry, Mass spectrometry Imaging, Fundamentals of Mass Spectrometry, Ionization Techniques, Mass Spectrometry Configurations and Separation Techniques, Chromatography and High Performance Liquid Chromatography (HPLC), Maintenance, Troubleshooting, Data Analysis and Experimentation in Mass Spectrometry, Mass Spectrometry in Proteome Research, Proteomics and its applications, Mass Spectrometry in Metabolomics and Lipidomics, Hyphenated Techniques (LC-NMR-MS, HPLC-ESI-MS, MC-ICP-MS, HPLC-ICP-MS, UPLC-Q-TOF/MS), Mass spectrometry in environmental analysis, Spectroscopy, Clinical application of mass spectrometry, UV, IR and Ion Spectroscopy, Nuclear Magnetic Resonance(NMR) Spectroscopy, Mass Spectrometry in Toxicology, Mass spectrometry in Analytical Science, Mass Spectrometry in Pharmaceutical Analysis,
Composition and Function of the Extracellular Matrix in the Human Body
The extracellular matrix (ECM) is an ensemble of non-cellular components present within all tissues and organs of the human body. The ECM provides structural support for scaffolding cellular constituents and biochemical and biomechanical support for those events leading to tissue morphogenesis, differentiation and homeostasis. Essential components of all ECMs are water, proteins and polysaccharides. However, their composition, architecture and bioactivity greatly vary from tissue to tissue in relation to the specific role the ECM is required to assume. This book overviews the role of the ECM in different tissues and organs of the human body.
Sarcopenia – Age-Related Muscle Wasting and Weakness
Some of the most serious consequences of aging are its effects on skeletal muscle. ‘Sarcopenia’, the progressive age-related loss of muscle mass and associated muscle weakness, renders frail elders susceptible to serious injury from sudden falls and fractures and at risk for losing their functional independence. Not surprisingly, sarcopenia is a significant public health problem throughout the developed world. There is an urgent need to better understand the neuromuscular mechanisms underlying age-related muscle wasting and to develop therapeutic strategies that can attenuate, prevent, or ultimately reverse sarcopenia. Significant research and development in academic and research institu...
Protein Purification Protocols
Hans Neurath has written that this is the second golden era of enzymology {Protein Science [1994], vol. 3, pp. 1734—1739); he could with justice have been more general and referred to the second golden age of protein chemistry. The last two decades have seen enormous advances in our understanding of the structures and functions of pro teins arising on the one hand from improvements and developments in analytical techniques {see the companion volume, Basic Protein and Peptide Protocols, in this series) and on the other hand from the tech nologies of molecular genetics. Far from turning the focus away from protein science, the ability to isolate, analyze, and express genes has increased inte...
Current Topics in Developmental Biology
This Series provides a comprehensive survey of the major topics in the field of developmental biology. The volumes are valuable to researchers in animal and plant development, as well as to students and professionals who want an introduction to cellular and molecular mechanisms of development. This year marks a major milestone for the Series as it completes its thirtieth year of publication, making it the longest-running forum for contemporary issues in developmental biology.
Protein Purification Protocols
The first edition of Protein Purification Protocols (1996), edited by Professor Shawn Doonan, rapidly became very successful. Professor Doonan achieved his aims of p- ducing a list of protocols that were invaluable to newcomers in protein purification and of significant benefit to established practitioners. Each chapter was written by an ex- rienced expert in the field. In the intervening time, a number of advances have w- ranted a second edition. However, in attempting to encompass the recent developments in several areas, the intention has been to expand on the original format, retaining the concepts that made the initial edition so successful. This is reflected in the structure of this se...
Cellular and Molecular Procedures in Developmental Biology
This volume incorporates practical methods at the level of molecular, cellular, and whole organism biology in vertebrate and invertebrate models. It presents straightforward protocols written step-by-step for state-of-the-art techniques with the emphasis on single-cell resolution procedures. - Provides straightforward, current protocols and critical appraisals - Includes diverse analysis of cellular and molecular techniques - Presents everything from whole-organ cultures to electrophysiological approaches - Details a variety of methods for interfering with gene function in various species - Offers multiple illusions of in situ hybridization, immunostaining, and apoptosis
Proteomics Mass Spectrometry Methods
Proteomics Mass Spectrometry Methods: Sample Preparation, Protein Digestion, and Research Protocols shares best practices collected across key laboratories and core facilities, taking the reader through key tactics for executing the most usual mass spectrometry experiments. Sections review research making use of MS proteomics experiments, focus on critical sample preparation, cover mammalian cell lines and samples from clinical tissue and biological fluids, discuss subcellular fractionation, provide methods for protein digestion both for in gel and in solution, and delve into key MS proteomics analysis protocols, including label-free LC-MS, TMT and iTRAQ labelled LC-MS, phosphorylation enric...
Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology
A major update of a best-selling textbook that introduces students to the key experimental and analytical techniques underpinning life science research.
