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The second edition of Experiments in Plant Tissue Culture makes available new information that has resulted from recent advances in the applications of plant tissue culture techniques to agriculture and industry. This comprehensive laboratory text takes the reader through a graded series of experimental protocols and also provides an introductory review of each topic. Topics include: a plant tissue culture laboratory, aseptic techniques, nutritional components of media, callus induction, organ formation, xylem cell differentiation, root cultures, cell suspensions, micropropagation, embryogenesis, isolation and fusion of protoplasts, haploid cultures, storage of plant genetic resources, secondary metabolite production, and quantification of procedures. This volume offers all of the basic experimental methods for the major research areas of plant tissue culture, and it will be invaluable to undergraduates and research investigators in the plant sciences.
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"Native to the New World, the potato was domesticated by Andean farmers, probably in the Lake Titicaca basin, almost as early as grain crops were cultivated in the Near East. Full of essential vitamins and energy-giving starch, the potato has proved a valuable world resource. Curious Spaniards took the potato back to Europe, from whence it spread worldwide. Today, the largest potato producer is China, with India not far behind. To tell the potato's story, Lang has done fieldwork in South America, Asia, and Africa."--Jacket.
Plant Growth and Development: A Molecular Approach presents the field of plant development from both molecular and genetic perspectives. This field has evolved at a rapid rate over the past five years through the increasing exploitation of the remarkable plant Arabidopsis. The small genome, rapid life cycle, and ease of transformation of Arabidopsis, as well as the relatively large number of laboratories that are using this plant for their research, have lead to an exponential increase in information about plant development mechanisms.In Plant Growth and Development: A Molecular Approach Professor Fosket synthesizes this flood of new information in a way that conveys to students the exciteme...
1 John H. Dodds The culture offragmen ts of plant tissue is not a particularly new science, in fact as long ago as 1893 Rechinger (1893) described the formation of callus on isolated fragments of stems and roots. The culture of plant tissues in vitro on a nutrient medium was performed by Haberlandt (1902), however, his attempts were unsuccessful because he chose too simple a medium that lacked critical growth factors. Over the last fifty years there has been a surge of development in plant tissue culture techniques and a host of techniques are now avail able (Dodds and Roberts, 1982). The major areas are as follows. Callus Culture Callus is a rather ill-dermed material. but is usually descri...