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Fifteen-year-old Amelina Scott lives in Cambridge with her dysfunctional family, a mysterious black cat, and an unusual girl who is imprisoned within the mirrors located in her house. When an unexpected message arrives inviting her to visit the Crystal Cottage, she sets off on a forbidden path where she encounters Ryder: a charismatic, perplexing stranger. With the help of a magical paint set and some crystal wizard stones, can Amelina discover the truth about her family? A unique, imaginative mystery full of magic-wielding and dark elements, Bloodstone is a riveting adventure for anyone interested in fantasy, mythology or the world of the paranormal.
The tale of a stumped policeman, a third man, and a well-placed shot. It’s 1984. Princess Diana has just given birth to her second child. The legendary comic Tommy Cooper has died on stage (quite literally). And Angus Lovejoy and Brian Finkle are gloriously oblivious to it all as they strive to enthral the nation with their television commercials for the advertising agency Gordon Deedes Rutter. But all is not as rosy as it might seem in the frenetic world of Soho. Following a disastrous presentation to a manufacturer of diarrhoea tablets, Lovejoy and Finkle let off steam by playing cricket for an old school friend - but in doing so, stumble upon a nest of vipers involving a gay vicar, a small-time antique fraudster, a photographer, and blackmail. There can only be one outcome and it’s going to entail murder.
This volume discusses different approaches towards understanding the brain plasticity, aging, and regeneration of the nervous system in model organisms. Chapters guide the reader through an overview of model organisms, setup of an aquatic system, Tol2-mediated transgenesis, CRISPR/Cas9-mediated gene knock-out and knock-in, Cas13d-mediated gene knockdown, tissue clearing and expansion methods for brain and spinal cord imaging, Histological techniques, chromatin Immunoprecipitation Sequencing (ChIP-Seq) analysis, single-cell RNA sequencing analysis, BrdU/EdU incorporation assays, brain and spinal cord injury in African killifish, and emerging technologies and future perspectives in African killifish research. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Authoritative and cutting-edge, Emerging Model Organisms aims to be a useful practical guide to researches to help further their study in this field.
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After Tom and Michelle Cleveland move into their recently built, modern townhouse, their housewarming party is disrupted when a drunken game with an Ouija board goes wrong and summonses a sinister poltergeist, Estelle, who died in 1904. Estelle makes her presence known in a series of terrifying events, culminating in her attacking Tom in his sleep with a knife. But, Estelle isn't alone. Who are the shadows lurking in the background - one in an old-fashioned slouch hat and the other, a soldier, carrying a rifle? After discovering their house has been built on the site of one of the original farms in Irene, Michelle becomes convinced that the answer to her horrifying visions lie in the past. She must unravel the stories of the three phantoms' lives, and the circumstances surrounding their untimely deaths during the Second Anglo Boer War, in order to understand how they are tied together and why they are trapped in the world of ghosts between life and death. As the reasons behind Estelle's malevolent behaviour towards Tom unfold, Michelle's marriage comes under severe pressure and both their lives are threatened.
In this landmark volume, an international group of scientists has synthesized their collective expertise and insight into a newly unified vision of insect societies and what they can reveal about how sociality has arisen as an evolutionary strategy. Jürgen Gadau and Jennifer Fewell have assembled leading researchers from the fields of molecular biology, evolutionary genetics, neurophysiology, behavioral ecology, and evolutionary theory to reexamine the question of sociality in insects. Recent advances in social complexity theory and the sequencing of the honeybee genome ensure that this book will be valued by anyone working on sociality in insects. At the same time, the theoretical ideas presented will be of broad-ranging significance to those interested in social evolution and complex systems.
Building upon the success of previous editions of the bestselling Handbook of Laboratory Animal Science, first published in 1994, this latest revision combines all three volumes in one definitive guide. It covers the essential principles and practices of Laboratory Animal Science as well as selected animal models in scientific disciplines where much progress has been made in recent years. Each individual chapter focuses on an important subdiscipline of laboratory animal science, and the chapters can be read and used as stand-alone texts, with only limited necessity to consult other chapters for information. With new contributors at the forefront of their fields, the book reflects the scienti...
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