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New Port Richey, Florida, like many cities between Jacksonville and Tampa, can thank Henry Plant's 1885 railroad for its phenomenal growth. Thirty-five miles northwest of Tampa, in West Pasco County, New Port Richey eventually hosted its own railway connection right through downtown. City planners constructed the community in a grid, naming north-south streets after Presidents and east-west streets after states. The arrival of the U.S. Post Office in 1915 confirmed this city's importance and put New Port Richey on the map. Hotels, banks, and businesses sprang up in the downtown area to serve those who came in search of a better life. Fishing on the Pithlachascotee River and in the Gulf of Mexico attracted many visitors, as did the construction of golf courses. Businessmen then and now recognized that this area had "that special something" to catch the attention and the hearts of people from all states north of Florida.
The success of laboratory experiments relies heavily on the technical ability of the bench scientist, with the aid of "tricks-of-the-trade", to generate consistent and reliable data. Regrettably, however, these invaluable "tricks-of-the-trade" are frequently omitted from scientific publications. This paucity of practical information relating to the conduct of laboratory bacteriology experiments creates a gaping void in the pertinent literature. Methods in Practical Laboratory Bacteriology fills this void. It provides detailed technical information that ensures that you achieve consistent and reliable data. The book addresses the aspects of bacterial fractionation and membrane characterization, the analysis of Lipopolysaccharides and the techniques of SDS-PAGE, immunoblotting, and ELISA. It also describes the methods used for detecting and quantifying bacterial resistance to antibiotics, and the analysis of bacterial chromosomes by pulsed-field gel electrophoresis (PFGE). Methods in Practical Laboratory Bacteriology also covers protocols for extracting the fingerprinting plasmids, as well as the use of non-radio labeled gene probes and ribosomal RNA gene probes.
The dramatic race to transplant the first human heart spanned two years, three continents and five cities against a backdrop of searing tension, scientific brilliance, ethical controversy, racial strife and emotional turmoil. It culminated in a terrifying moment in the early hours of 3 December 1967 when, in a cramped operating theatre in a Cape Town hospital, Professor Chris Barnard stared into an empty cavity from which he had just removed a heart. He knew that he had only minutes left to make history and save the life of a 55-year-old man by filling the gaping hole in his chest with a heart which had just been beating inside a 25-year-old woman. Every Second Countsis the story of this gripping race to conquer the greatest of medical challenges. It also reveals the truth about the man at the centre of it all, whose turbulent life story was just as gripping. The kind of true story that would be dismissed as far-fetched if presented as fiction, it combines an utterly compelling portrait of cutting-edge science with raw human drama, and shows how the course of medicine itself was changed for ever.
Aldous Huxley decried "the horrors of modern 'pleasure,'" or the proliferation of mass produced, widely accessible entertainment that could degrade or dull the mind. He and his contemporaries, including James Joyce, T. S. Eliot, Gertrude Stein, D. H. Lawrence, and Jean Rhys, sought to radically redefine pleasure, constructing arduous and indirect paths to delight through their notoriously daunting work. Laura Frost follows these experiments in the art of unpleasure, connecting modernism's signature characteristics, such as irony, allusiveness, and obscurity, to an ambitious attempt to reconfigure bliss. In The Problem with Pleasure, Frost draws upon a wide variety of materials, linking inter...
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This book is designed to complement the academic and experiential training of therapists. Written by experienced practitioners, it gives trainees a practical insight into the ways in which group analysts may tackle difficult situations, allowing them to understand more fully the nature of intervention right from the beginning of their training.