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PCR Primer Design
This third edition provides new and updated chapters on design PCR primers for successful DNA amplification. Chapters are divided into seven parts, including primer design strategies for quantitative PCR, genotyping, multiplex PCR, in silico PCR primer design, and primer design to identify plant and animal viruses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, PCR Primer Design, Third Edition aims to be useful for various fields of molecular biology, including biotechnology, molecular genetics, and recombinant DNA technology.
Forty Years on
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The Poor Man's Primer Manual
This work details the production of primers from a commerical prospective and then in the final chapter gives comprehensive instructions on how to produce small quantities of primers in a laboratory or home environment. The book covers the process of creating the necessary materials (priming mixes), primer component reconditioning, and development and procurement of the basic tools. These topics are detail in a manner that the student can readily understand both on a practical and academic level.
Transcontinental Dialogues
Transcontinental Dialogues brings together Indigenous and non-Indigenous anthropologists from Mexico, Canada, and Australia who work at the intersections of Indigenous rights, advocacy, and action research. These engaged anthropologists explore how obligations manifest in differently situated alliances, how they respond to such obligations, and the consequences for anthropological practice and action. This volume presents a set of pieces that do not take the usual political or geographic paradigms as their starting point; instead, the particular dialogues from the margins presented in this book arise from a rejection of the geographic hierarchization of knowledge in which the Global South co...
PCR Primer
A guide to the complexities of the polymerase chain reaction that begins at an accessible level and then moves to more advanced levels of application. First, the practical requirements for performing PCR and other amplification techniques in the lab are introduced, and then the basic aspects of the technique are explained by exploring issues such as sample preparation, primer design, efficiency, detection of products, and quantitation. Protocols for a wide range of PCR and amplification techniques are presented for cloning, sequencing, mutagenesis, library construction and screeing, exon trapping, differential display, and expression; these include RT-PCR, RNA PCR, LCR, multiplex PCR, panhandle PCR, capture PCR, expression PCR, 3' and 5' RACE, in situ PCR, and ligation-mediated PCR. Plastic comb-binding. Annotation copyright by Book News, Inc., Portland, OR
Sequence Analysis Primer
Covers the basic computer analyses used for new DNA sequences and attempts to provide the researcher with the necessary background in order to understand and use efficiently these programs.
The Visual Narrative Reader
"Not all narrative is textual. Images, sequential or non-sequential, have made meaning and told stories since the first cave art and through to the latest 3D movies. This book understands that analyzing this form of narrative is important and fundamental to a complete theory of discourse. Cohn's Visual Narrative Reader explores many forms of image-based narratives. It shows how meaning and sequence is produced and how to approach the discourse analysis involved. Contemporary research on the visual language of sequential images have been scattered across several disciplines: linguistics, psychology, anthropology, art education, and others. Only recently has this disparate research begun to be...
Culture & Text
In an exciting departure in the growing field of discourse analysis, Culture & Text combines a fresh approach to theory with exemplary demonstrations of interdisciplinary analysis. Despite its emphasis on text, cultural studies has kept most forms of discourse analysis at arm's length. Positioned at the conjunction of linguistic and poststructuralist approaches to discourse analysis, this book argues for a textual metalanguage for cultural studies and for a reevaluation of methodology.
