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Live Cell Imaging
Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a DVD containing movies of living cells in action, created by investigators using the imaging techniques discussed in the book. The editors, David Spector and Robert Goldman, whose previous book was Cells: A Laboratory Manual,are highly respected investigators who have taught microscopy courses at Cold Spring Harbor Laboratory, the Marine Biology Laboratory at Woods Hole, and Northwestern University.
Sign Wars
Television has become so saturated with commercials that it is difficult at times to tell the different images apart, much less remember or care about them. But, on closer look, television commercials can tell us a great deal about the interplay of market forces, contemporary culture, and corporate politics. This book views contemporary ad culture as an ever-accelerating war of meaning. The authors show how corporate symbols or signs vie for attention-span and market share by appropriating and quickly abandoning diverse elements of culture to differentiate products that may be in themselves virtually indistinguishable. The resulting "sign wars" are both a cause and a consequence of a media culture that is cynical and jaded, but striving for authenticity. Including more than 100 illustrations and numerous examples from recent campaigns, this book provides a critical review of the culture of advertising. It exposes the contradictions that stem from turning culture into a commodity, and illuminates the impact of television commercials on the way we see and understand the world around us.
The Cytoskeleton
The cytoskeleton is the intracellular filament system that controls the morphology of a cell, allows it to move, and provides trafficking routes for intracellular transport. It comprises three major filament systems-actin, microtubules, and intermediate filaments-along with a host of adaptors, regulators, molecular motors, and additional structural proteins. This textbook presents a comprehensive and up-to-date view of the cytoskeleton, cataloguing its many different components and explaining how they are functionally integrated in different cellular processes. It starts by laying out the basic molecular hardware, before describing in detail how these components are assembled in cells and linked to neighboring cells and the extracellular matrix to maintain tissue architecture. It then surveys the roles of the cytoskeleton in processes such as intracellular transport, cell motility, signal transduction, and cell division. The book is thus essential reading for students learning about intracellular structure. It also represents a vital reference for all cell and developmental biologists working in this field.
Devavāṇīpraveśikā
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Cellular and Molecular Biology of Intermediate Filaments
Research activity on intermediate filaments (IF) has increased dramatically over the past decade. For the most part, this surge of interest is due to their identification as ubiquitous constituents of the cytoskeleton and karyoskeleton (nuclear matrix) of eukaryotic cells and the fact that we know very little regarding their functions. In sharp contrast to the other major cytoskeletal systems, microfilaments and microtubules, IF exhibit a high degree of heterogeneity with regard to their protein subunit composition. Indeed, one can only marvel at the number of different IF polypeptides, their associated proteins (IFAP) and, consequently, the number of genes involved in encoding the multiple ...
Basic Methods in Microscopy
This manual contains selected material from Cells - a Laboratory Manual, as well as two chapters from Live Cell Imaging. It includes sections on microscopy, and on preparing and labelling specimens for microscopy.
Ramayana
Rama, the crown prince of the City of Ayodhya, is a model son and warrior. He is sent by his father the king to rescue a sage from persecution by demons, but must first kill a fearsome ogress. That done, he drives out the demons, restores peace, and attends a tournament in the neighboring city of Mithila; here he bends the bow that no other warrior can handle, winning the prize and the hand of Sita, the princess of Mithila.Valm'ki's Ramßyana is one of the two great national epics of India, the source revered throughout South Asia as the original account of the career of Rama, ideal man and incarnation of the great god Vishnu. The first book, "Boyhood," introduces the young hero Rama and sets the scene for the adventures ahead. It begins with a fascinating excursus on the origins and function of poetry itself.For more on this title and other titles in the Clay Sanskrit series, please visit http://www.claysanskritlibrary.org
Intermediate Filament Cytoskeleton
Intermediate filaments are a large family of proteins that are the cytoskeletal elements involved in a number of skin, liver, neuromuscular, cardiac, eye and hair diseases. Intermediate filament genes are regulated in a tissue-and cell type-specific manner and their polymerized protein products protects the cells and tissue they are part of against a variety of mechanical and nonmechanical stresses. This book provides a comprehensive resource of methodology essentials, describing a variety of essential tools and assays for studying intermediate filaments. The book provides user-friendly advice and protocols covering all aspects of intermediate filaments including protein isolation and struct...
