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Viral Gene Techniques
  • Language: en
  • Pages: 436

Viral Gene Techniques

  • Type: Book
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  • Published: 1995-11-14
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  • Publisher: Elsevier

Viral Gene Techniques is a practical laboratory guide to current techniques of molecular biology and genetics. The volume is concerned with methods for the analysis of viral genes and chromosomes: DNA viruses and RNA viruses including HIV are discussed.* Methods presented for ease of use and reasdy adaptation to new systems* Detailed experimental protocols included for:* Viral vectors - construction and use of DNA virus vectors (adenovirus, adeno-associated virus, vaccinia virus, Epstein - Barr virus)* DNA viruses - virus/host interactions, viral chromosomes , transcription regulation (viruses discussed include herpes simplex, hepatitis B, SV40, JC, Epstein-Barr, adenovirus)* Human Immunodeficiency Virus / retroviruses -quantitation of HIV-1 virus stock and RNA, retrovirus reverse transcription / integration, retrovirus-mediated cell fusion, use as cell lineage markers* RNA viruses - RNA virus assembly, analysis of RNA genomes, assays for RNA-binding proteins (viruses discussed include poliovirus, influenza virus, hepatitis delta virus)

Progress in Nucleic Acid Research and Molecular Biology
  • Language: en
  • Pages: 291

Progress in Nucleic Acid Research and Molecular Biology

Progress in Nucleic Acid Research and Molecular Biology

DNA Methylation
  • Language: en
  • Pages: 581

DNA Methylation

  • Type: Book
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  • Published: 2013-11-11
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  • Publisher: Birkhäuser

The occurrence of 5-methylcytosine in DNA was first described in 1948 by Hotchkiss (see first chapter). Recognition of its possible physiologi cal role in eucaryotes was first suggested in 1964 by Srinivasan and Borek (see first chapter). Since then work in a great many laboratories has established both the ubiquity of 5-methylcytosine and the catholicity of its possible regulatory function. The explosive increase in the number of publications dealing with DNA methylation attests to its importance and makes it impossible to write a comprehensive coverage of the literature within the scope of a general review. Since the publication of the 3 most recent books dealing with the subject (DNA meth...

Molecular Basis of Viral and Microbial Pathogenesis
  • Language: en
  • Pages: 194

Molecular Basis of Viral and Microbial Pathogenesis

Elucidation of the mechanisms of pathogenesis underlying the diseases caused by viruses and bacteria has fascinated scientists for many years in two ways. Firstly, these pathogenic agents represent relatively sim ple biological systems for the study of basic biological processes such as replication, gene regulation, genetic variability and host-pathogen interactions. Secondly, process in this field is valuable in a practi cal sence, since it can help in the control of these diseases. The avail ability of new genetic and immunological techniques, especially recom binant DNA methods and monoclonal antibody technology, has provided powerful tools for unravelling the genetic, biochemical and imm...

Advances in Cancer Research
  • Language: en
  • Pages: 375

Advances in Cancer Research

ADVANCES IN CANCER RESEARCH is a biannual publication that includes timely reviews on the most cutting-edge issues in cancer research. Volume 66 contains encompassing overviews of p53 and its role in both breast cancer and in the cell cycle. Approximately 50% of all human tumors involve mutations of the p53 gene, suggesting that proper understanding of its properties and mechanisms could offer real hope for finding successful clinical therapy. Other themes presented in Volume 66 include cyclins and cyclin-dependent kinases in the cell cycle. Approximately 50% of all human tumors involve mutations of the p53 gene, suggesting that proper understanding of its properties and mechanisms could off...

Cell Membrane
  • Language: en
  • Pages: 454

Cell Membrane

This publication presents the structure and function of biological membranes to improve the understanding of cells in both normal and pathogenic states. Recently, vast amounts of new information have been accumulated, especially about pathological conditions, and there is now much evidence correlating genotypes and phenotypes in normal and disease states. This book surveys the most recent findings in research on the molecular biology, biochemistry, and genetics of the membranes of human red blood cells.

Advances in Virus Research
  • Language: en
  • Pages: 368

Advances in Virus Research

Advances in Virus Research

Springfield (Sangamon County, Illinois) City Directory
  • Language: en
  • Pages: 362

Springfield (Sangamon County, Illinois) City Directory

  • Type: Book
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  • Published: 1882
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  • Publisher: Unknown

None

Integration and Excision of DNA Molecules
  • Language: en
  • Pages: 147

Integration and Excision of DNA Molecules

The topic of this years' ~osbach Colloquium was DNA integration. We have tried to bring together experts from different fields of research who are studying natural processes by which DNA molecules from differ ent sources are linked. It has been known for a long time that such linkage occurs between the chromosomes of bacteriophages and plasmids on the one hand and the chromosome of the bacterial host on the other. This process has been especially well studied in bacteriophage A. Since it is controlled in a complicated way, we began with a lecture by M. ptashne on these regulatory processes. H. Nash described the inte gration of bacteriophage A into the bacterial chromosome. To put this site-specific process into perspective, G. Mosig lectured on genetic recombination in prokaryotes in general and K. Murray described the use of bacteriophage A as an artificial vector for genetic engineering. A different kind of bacteriophage integration is shown by bacteriophage Mu, which is much less specific in its choice of an integration site than A. The properties of this phage were described by P. van de Putte.

Current Topics in Microbiology and Immunology / Ergebnisse der Mikrobiologie und Immunitätsforschung
  • Language: en
  • Pages: 178

Current Topics in Microbiology and Immunology / Ergebnisse der Mikrobiologie und Immunitätsforschung

The processes involved in herpesvirus replication, latency, and oncogenic transformation, have, in general, been rather poorly defined. A primary reason for this is the size and complexity of the herpesvirus genome. Undoubtedly, a better understanding of the functions of the viral genome in infected and transformed cells will be achieved through studies with temperature-sensitive (ts) mutants of herpesviruses since, theoretically, any essential gene function can be affected by mutants of this type. A. The Herpesviruses A consideration of the genetic analysis of members of the herpesvirus group necessitates a description, albeit brief, of the properties of the group and, most importantly, of ...