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The potential of 9 bacterial and 6 fungal isolates to protect wood against wood staining microorganisms was studied using small sapwood samples of unseasoned Ponderosa pine [Pinus ponderosa Laws] sapwood. Bacillus subtilis Cohn, isolate 733 A, Gliocladium virens J.H. Miller, J.E. Giddens & A.A. Foster isolate H 3 and Pseudomonas putida Migula isolate A-12 were identified as promising biological agents. The effect of media on the ability of B. subtilis # 733 A and B. subtilis ATCC 6633 to protect Ponderosa pine specimens from microbial discoloration was evaluated. A media preference was demonstrated with each of the species tested, which enhanced bioprotectant capability. B. subtilis # 733 A ...