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Hybridization of Somatic Cells
Since its discovery in I960, the hybridization of somatic cells has evolved from a biological curiosity into an analytical method that today underlies nearly all investigations of the genetic aspects of various biological phenomena. As an eyewitness to this development from its inception forward, Boris Ephrussi here relates the history of somatic hybridization and the formation of its methodology, lie follows with a discussion of the characteristics and properties of the resultant hybrid cells. Together, these topics comprise an authoritative introduction to the principles of the technique. Dr. Ephrussi proceeds to an examination in greater detail of three specific areas of biological resear...
Cell Hybrids
Cell Hybrids summarizes the methodology of cell fusion-the fusion of human, animal, and plant cells of different origins to produce cell hybrids-and surveys the main applications and current findings of the hybridization technique. The book opens with a chapter on the history cell hybridization. This is followed by separate chapters on spontaneous cell fusion, virus-induced cell fusion, the cell fusion mechanism, regulation of DNA synthesis and mitosis in heterokaryons and homokaryons, and regulatory events which occur when two cells with different nuclear activity and/or phenotype are fused with each other. Subsequent chapters deal with methods used in preparing various cell fragments and some of their properties and uses in fusion experiments; isolation of growing hybrid cells; chromosome patterns and phenotypic expression in hybrid cells; cell organelles in hybrid cells; analysis of malignancy by cell hybridization. The final chapters discuss the use of somatic cell hybridization to analyze the interaction between a number of viruses and their host cells; and the use of plant cell hybrids.
Distant Hybridization of Crop Plants
Wild taxa are invaluable sources of resistance to diseases, insects/ pests, nematodes, temperature extremes, salinity and alkalinity stresses, and also of nutritional quality; adaptation; genetic diversity and new species. Utilization of wild relatives of a crop depends largely upon its crossability relations with cultivated varieties. Sev eral wild species are not crossable with the commercial cultivars due to various isolation barriers. Furthermore, in a few cases, hybridiza tion is possible only in one direction and reciprocal crosses are not successful, thus depriving the utilization of desired cytoplasm of many species. However, techniques have been developed to over come many barriers ...
Somatic Hybridization in Crop Improvement II
This richly illustrated volume describes how somatic hybrids can contribute to the improvement of crops. It comprises 24 chapters dealing with interspecific and intergeneric somatic hybridization and cybridization, providing valuable tools for plant breeders.
Somatic Hybridization in Crop Improvement I
Thirty-five chapters on various aspects of fusion of plant protoplasts and somatic hybridization deal with the regeneration of interspecific and intergeneric somatic hybrids and cybrids in various plants: cereals, grasses, legumes, potato, tomato, eggplant, lettuce, Brassica, Datura, Hyoscyamus, Nicotiana, Catharanthus, Rauwolfia, Citrus, Poncirus, Prunus, Pyrus, Populus, algae, bryophytes, and ferns. The implications of somatic hybridization in gene transfer in wide crosses and for the induction of genetic variability in various crops are discussed. The book is an invaluable source of information for advanced students, teachers, and research scientists in the field of plant breeding, genetic engineering, plant tissue culture, and general plant biotechnology.
