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In the relatively short period since Cryptosporidium was recognised as a human pathogen, and that it could be transmitted in water as well as directly between animals and people, it has been the subject of intense investigations. Its status as an opportunistic pathogen, especially in AIDS patients, and the lack of effective anti-cryptosporidial drugs have served to emphasise the public health importance of this organism. This has to some extent overshadowed the fact that Cryptosporidium is also an important pathogen of domestic animals and wildlife. In recent years, the application of molecular biology and culture techniques have had an enormous impact on our understanding of the aetiologica...
Cryptosporidiosis is a parasitic disease that strikes humans and domestic animals, ranging from isolated occurrences to epidemics. Infection caused by this parasite can lead to enteric disease and other debilitating conditions that can be life-threatening in immunocompromised animals and humans. Increased recognition of the seriousness of cryptosporidiosis has aroused the concern of public health, medical, veterinary, and research communities. Cryptosporidium and Cryptosporidiosis is the only text to specifically address this disease-its diagnosis, epidemiology, waterborne events, prevention and treatment, immunity, biochemistry, cultivation, laboratory animal models, and molecular biology. This book summarizes data on taxonomy, life cycles, morphology, host species, and control methods of cryptosporidiosis. It addresses the latest industry trends-developing disinfectants, medications, and water purification methods-and also academic trends-diagnostics, immune responses, biochemistry, and molecular biology research.
This book encompasses broad aspects of Cryptosporidium research with established methods that have been improved and expanded over the years as well as recent cutting-edge techniques. Within this collection are numerous molecular methods as well as protocols for genotyping and diagnostics, while also including room for in vitro cell culture techniques to address the issues with growing this difficult organism continuously. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cryptosporidium: Methods and Protocols serves as an idea guide to the inherent challenges of working with cryptosporidial parasites to provide a foundation for new investigators to build upon. The chapter “Accessing Cryptosporidium Omic and Isolate Data via CryptoDB.org” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
This book provides a comprehensive review of the current state of analytical techniques for the detection of Cryptosporidium, as well as looking at likely future developments.
Giardia and Cryptosporidium are both parasites of considerable global interest due to the gastrointestinal problems the organisms can cause in humans as well as domestic and wild animals. This book presents a comprehensive overview of recent research. Chapters discuss topics from taxonomy, nomenclature and evolution to molecular epidemiology, advances in diagnostics and zoonotic, human and animal health issues.
Although widely recognized as an important waterborne pathogen, Cryptosporidium spp. can also be transmitted by contamination of food. The same properties of this protozoan parasite that mean that water is an excellent transmission vehicle are also important for foodborne transmission. These include the low infective dose, the high number of oocysts that are excreted, and the robustness of these transmission stages. However, many more outbreaks of waterborne cryptosporidiosis have been reported than foodborne outbreaks. This is probably partly due to epidemiological tracing being much more difficult for foodborne outbreaks than waterborne outbreaks, and the number of persons exposed to infec...
This book attempts to provide a broad coverage of current information needed by public health workers, physicians, veterinarians, parasitologists, technicians, and various biologists who encounter or work with the parasitic disease Cryptosporidium.
'... This volume provides most complete and balanced coverage of essential aspects of the pathogens as well as the diagnosis and clinical correlations of the disease they cause. It is set to become a valuable refrence for parasitologists, protozoologists, molecular biologists, clinical mecrobiologists, epidemiologists and specialists in infectious diseases.'