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The ELISA Guidebook
There have been very few developments that markedly affect the need to greatly revise the text from the last version of this book. This is testament to the fact that hetero- neous enzyme-linked immunosorbent assays (ELISA) provide ideal systems for dealing with a wide range of studies in many biological areas. The main reason for this success is test flexibility, whereby reactants can be used in different combinations, either attached passively to a solid phase support or in the liquid phase. The exploitation of the ELISA has been increased through continued development of specifically produced reagents, for example, monoclonal and polyclonal antibodies and peptide antigens coupled with the ...
Elisa: Theory and Practice. Methods in Molecular Biology, Volume 42
"ELISA: Theory and Practice" introduces to scientists at all levels of expertise the principles of the most commonly used assay technique known as the Enzyme Linked Immunosorbent Assay. The book provides readers with full descriptions of the basic systems that make ELISA one of the most powerful techniques in science today, and also examines in detail the data obtained by ELISA and their analysis and actual manipulation. "ELISA: Theory and Practice" is designed not only to train novices in the science of ELISA, but also to aid investigators experienced in any of the biological sciences in performing independently assays of antibodies and antigens. Mastery of the book's contents will allow readers to fully appreciate exactly how and why assays function, as well as permit the efficient development of individual assays that are both rapid and accurate.
ELISA
This volume provides an understanding of how an immunoassay works, detailing the strengths, weaknesses, pitfalls. Chapters guide readers on how and when to appropriately utilize this powerful tool, examples of where the ELISA or similar immunoassay formats are currently being used, and newer techniques that may have a significant impact on future applications. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, ELISA: Methods and Protocols is a valuable resource for both novice and expert scientists in this developing field.
The Immunoassay Handbook
The fourth edition of The Immunoassay Handbook provides an excellent, thoroughly updated guide to the science, technology and applications of ELISA and other immunoassays, including a wealth of practical advice. It encompasses a wide range of methods and gives an insight into the latest developments and applications in clinical and veterinary practice and in pharmaceutical and life science research. Highly illustrated and clearly written, this award-winning reference work provides an excellent guide to this fast-growing field. Revised and extensively updated, with over 30% new material and 77 chapters, it reveals the underlying common principles and simplifies an abundance of innovation. The...
A Practical Guide to ELISA
This practical and readable handbook is written for the non-specialist and first-time users of ELISA - enzyme-linked immunosorbent assay. Basic aspects of immunoassays and assay design are discussed. The explanation of ELISA, describing the reagents used, how to calibrate the equipment and how to interpret results, encourages understanding of this technique. The chapter on "Trouble Shooting" enables the user to identify and rectify problems, thus increasing his knowledge and ability so that the recipe section at the end of the book may be used to full advantage.
ELISA
ELISA: Theory and Practice introduces to scientists at all levels of expertise the principles of the most commonly used assay technique known as the Enzyme Linked Immunosorbent Assay. The book provides readers with full descriptions of the basic systems that make ELISA one of the most powerful techniques in science today, and also examines in detail the data obtained by ELISA and their analysis and actual manipulation. ELISA: Theory and Practice is designed not only to train novices in the science of ELISA, but also to aid investigators experienced in any of the biological sciences in performing independently assays of antibodies and antigens. Mastery of the book's contents will allow readers to fully appreciate exactly how and why assays function, as well as permit the efficient development of individual assays that are both rapid and accurate.
ELISA (Enzyme-Linked Immunsorbent Assay) Practical Report
Essay aus dem Jahr 2015 im Fachbereich Biologie - Humanbiologie, Sprache: Deutsch, Abstract: The Enzyme-Linked Immunosorbent assay (ELISA) is a test that is conducted to identify the substance under study with the help of antibodies and changing color elements. It is the "wet-lab" oriented analytic biochemistry assay that consume enzyme immunoassay to detect the existence of a substance (antigen) in a liquid sample. It is of great help in medicine and plant pathology as a diagnostic tool and quality control check. ELISA test involves one antibody according to the need of the test, where the antigen is immobilized on the solid plate. The method is done specifically and nonspecifically. The report involves the description and construction of two different ELISAs.
Elisa
"In September 1971, Swiss scientists Eva Engvall and Peter Perlmann published a communication in the journal Immunochemistry on enzyme-linked immunosorbent assay (ELISA) as a quantitative assay of immunoglobulin G. They have modified radioimmunoassay (RIA) by replacing the radioactive iodine with enzymes and used in determining the levels of IgG in rabbit serum. Fifty years after, ELISA is still the most commonly used technique in measuring peptides, proteins, antibodies, antigens, and hormones in various samples. This plate-based assay is a very versatile laboratory technique and offers many advantages to laboratory scientists and researchers such as generating quick results, simple protoco...
ELISA Techniques
Techniques in Diagnostic Pathology is a new series aimed at clinical and research pathologists which describes and critically evaluates a wide range of methods used in diagnostic pathology. Detailing essential steps and procedures not usually in primary reference material, the series encompasses well established methods as well as newer molecular techniques in such a way as to encourage their use in routine laboratory diagnosis. Volume 2 focuses on enzyme linked immunosorbent assays and details a range of techniques ELISA for antibody detection in infectious and autoimmune diseases ELISA for microbial antigen detection ELISA for antigen detection in cancer, degenerative diseases and environmental health Quantification of DNA/RNA hybridization products using ELISA ELISA for simultaneous detection of microbial antigen and microbial DNA Clinical immunologists, microbiologists, pathologists, and molecular biologistswill find this volume a valuable benchtop guide.
