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Directed Evolution Library Creation
  • Language: en

Directed Evolution Library Creation

  • Type: Book
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  • Published: 2010-11-10
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  • Publisher: Humana Press

Biological systems are very special substrates for engineering—uniquely the products of evolution, they are easily redesigned by similar approaches. A simple algorithm of iterative cycles of diversification and selection, evolution works at all scales, from single molecules to whole ecosystems. In the little more than a decade since the first reported applications of evolutionary design to enzyme engineering, directed evolution has matured to the point where it now represents the centerpiece of industrial biocatalyst development and is being practiced by thousands of academic and industrial scientists in com- nies and universities around the world. The appeal of directed evolution is easy ...

Directed Enzyme Evolution
  • Language: en
  • Pages: 381

Directed Enzyme Evolution

Directed evolution comprises two distinct steps that are typically applied in an iterative fashion: (1) generating molecular diversity and (2) finding among the ensemble of mutant sequences those proteins that perform the desired fu- tion according to the specified criteria. In many ways, the second step is the most challenging. No matter how cleverly designed or diverse the starting library, without an effective screening strategy the ability to isolate useful clones is severely diminished. The best screens are (1) high throughput, to increase the likelihood that useful clones will be found; (2) sufficiently sen- tive (i. e. , good signal to noise) to allow the isolation of lower activity c...

Progress and Prosperity
  • Language: en
  • Pages: 292

Progress and Prosperity

Progress & Prosperity focuses on the shift in Chinese cities from building for construction's sake to building for progress. As urban development shifts from quantity-driven to quality-driven, the volume explores whether this Chinese metamorphosis can serve as a blueprint for cities worldwide.

Directed Enzyme Evolution: Advances and Applications
  • Language: en
  • Pages: 286

Directed Enzyme Evolution: Advances and Applications

  • Type: Book
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  • Published: 2017-02-14
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  • Publisher: Springer

This book focuses on some of the most significant advances in enzyme engineering that have been achieved through directed evolution and hybrid approaches. On the 25th anniversary of the discovery of directed evolution, this volume is a tribute to the pioneers of this thrilling research field, and at the same time provides a comprehensive overview of current research and the state of the art. Directed molecular evolution has become the most reliable and robust method to tailor enzymes, metabolic pathways or even whole microorganisms with improved traits. By mirroring the Darwinian algorithm of natural selection on a laboratory scale, new biomolecules of invaluable biotechnological interest ca...

Not My Will
  • Language: en
  • Pages: 269

Not My Will

Eleanor's secret love for Chad could mean losing her inheritance and giving up a life-long dream. Will she follow her own will, or make the hard choice to submit her life to Christ's leadership? Now available with a contemporary new look, Not My Will is a classic story of love, loss, and surrender, with more than 500,000 copies sold.

The Amsterdam Agenda
  • Language: en
  • Pages: 168

The Amsterdam Agenda

From climate change to migration, all across the world cities are having to come to terms with contemporary challenges for which there are few easy answers. The Amsterdam Agenda invites 12 urban thinkers to share their knowledge, insights, and vision for the future of cities in light of today's ongoing transformations. Be it the repercussions of over-tourism, alternative forms of civic engagement, or incorporating new technologies into existing infrastructure, these are urgent issues with global impact. Based on a 2018 lecture series at the Amsterdam Academy of Architecture, The Amsterdam Agenda brings together the views of today's brightest names from the fields of architecture, planning, a...

New Enzymes for Organic Synthesis
  • Language: en
  • Pages: 256

New Enzymes for Organic Synthesis

Isolated enzymes are known as useful for synthesising of complex molecules with more and more large scale applications in recent years. These six reviews show different steps in the development of enzymatic syntheses for useful compounds. Screening of novel enzymes, their biochemical characterisation as well as reaction engineering approaches in order to increase their performance are treated in three papers. Then the use of protein design as a tool for the directed improvement of reactivity and selectivity of biocatalysts is described in two reviews. The supply of sufficient quantities of biocatalysts must not be neglected: fluidized bed adsorption as an emerging technology for the simple and fast purification of biomolecules is presented in the light of its potential in large scale supply of enzyme catalysts.

Transition Metal-Catalyzed Carbene Transformations
  • Language: en
  • Pages: 450

Transition Metal-Catalyzed Carbene Transformations

Presents an up-to-date overview of the rapidly growing field of carbene transformations Carbene transformations have had an enormous impact on catalysis and organometallic chemistry. With the growth of transition metal-catalyzed carbene transformations in recent decades, carbene transformations are today an important compound class in organic synthesis as well as in the pharmaceutical and agrochemical industries. Edited by leading experts in the field, Transition Metal-Catalyzed Carbene Transformations is a thorough summary of the most recent advances in the rapidly expanding research area. This authoritative volume covers different reaction types such as ring forming reactions and rearrange...

Artificial Metalloenzymes and MetalloDNAzymes in Catalysis
  • Language: en
  • Pages: 431

Artificial Metalloenzymes and MetalloDNAzymes in Catalysis

An important reference for researchers in the field of metal-enzyme hybrid catalysis Artificial Metalloenzymes and MetalloDNAzymes in Catalysis offers a comprehensive review of the most current strategies, developed over recent decades, for the design, synthesis, and optimization of these hybrid catalysts as well as material about their application. The contributors—noted experts in the field—present information on the preparation, characterization, and optimization of artificial metalloenzymes in a timely and authoritative manner. The authors present a thorough examination of this interesting new platform for catalysis that combines the excellent selective recognition/binding properties...

A Practical Guide to Protein Engineering
  • Language: en
  • Pages: 213

A Practical Guide to Protein Engineering

This textbook introduces readers in an accessible and engaging way to the nuts and bolts of protein expression and engineering. Various case studies illustrate each step from the early sequence searches in online databases over plasmid design and molecular cloning techniques to protein purification and characterization. Furthermore, readers are provided with practical tips to successfully pursue a career as a protein engineer. With protein engineering being a fundamental technique in almost all molecular biology labs, the book targets advanced undergraduates and graduate students working in molecular biology, biotechnology and related scientific fields.