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Engineering of Surface Proteins in Extracellular Vesicles for Tissue-Specific Targeting
Extracellular vesicles (EVs) have in the recent decades gained an important stand as vehicles enabling cell-to-cell transport and communication. With the advanced development towards their clinical use and increasing versatility of potential applications, improving their tissue-specific targeting in order to enhance their functionality in drug delivery opened as a challenging engineering field. In the past, the question of specific intercellular contact has been addressed by decoration of the EV surface with agents able of specific target recognition. An attractive possibility here is the modification of strongly overexpressed EV surface marker proteins towards recognition of target cells. As these proteins are involved in a plethora of biological functions in EV biogenesis, cargo targeting and intercellular transfer, a minimal impact on protein architecture upon modifications is desirable, which would also increase the stability of the exosomal preparation intended for therapeutic use. This chapter focuses on the possibilities of engineering of the EV marker proteins towards antigen-recognition units broadly applicable to endow EVs with tissue-targeting functionality.
Genotype Phenotype Coupling
This detailed new edition broadens the scope of the first edition by moving beyond classical display technologies. This book explores methodologies for the generation of natively paired antibody libraries, single cell technologies, alternative scaffolds, and in silico antibody sequence assessments are described. The application of these methods may allow for a generation of improved therapeutics and diagnostic reagents in a shorter time frame. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Genotype Phenotype Coupling: Methods and Protocols, Second Edition serves as an ideal guide for researchers seeking to expand their knowledge of antibody-based therapeutics.
Yeast Surface Display
This detailed volume explores a wide variety of applications of yeast surface display, an extensively used protein engineering technology. Beginning with detailed protocols for the construction and efficient selection/screening of yeast surface display libraries, as well as for the analysis of individual yeast-displayed protein variants, the book continues with protocols describing the selection of yeast surface display libraries for binding to mammalian cells or to extracellular matrix as well as protocols for a broad spectrum of specialized yeast surface display applications, demonstrating the versatility of this display platform. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible methodologies, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Yeast Surface Display serves as a comprehensive resource that enables the implementation of this powerful and versatile technique in virtually any molecular biology laboratory, even in the absence of any prior yeast surface display experience.
Ethnobotany and Ethnopharmacology of Medicinal and Aromatic Plants
Medicinal and aromatic plants are beneficial to human health. Plant-derived molecules possess biological activities that can be used to prevent many infectious diseases and metabolic disorders. Ethnobotany and Ethnopharmacology of Medicinal and Aromatic Plants summarizes techniques and methods used to study the biological activities of plant-derived extracts and compounds to study ethnobotanical and ethnopharmacological features of medicinal and aromatic plants. This book: Includes computational approaches to study the pharmacological properties of biomolecules in medicinal and aromatic plants. Details methods in ethnopharmacology including chromatographical and analytical techniques. Demons...
Current Topics in Biochemical Engineering
Genetic and cellular technologies in life science have recently achieved remarkable progress, and thus the roles of biochemical engineers have also been changed to incorporate the use of new technology. Therefore, this book deals with current topics in biochemical engineering. The chapters of this book discuss research that has introduced artificial enzymes, kinetic models in bioprocessing, a small-scale production process, and production of energy with microbial fuel. These chapters offer novel ideas for the production of effective compounds and energy. Moreover, other research has introduced the production technology of stem cells and biomedical processes using nanoshells and extracellular vesicles. These chapters will provide novel ideas to produce effective compounds and develop therapies for various diseases.
Regulating Global Climate Change
For some years now, growing scientific warnings have continued to strengthen the belief that an unprecedented global warming is underway, and that only an urgent system-wide transformation can avoid climate disaster. In his June 2, 2022 address to the Stockholm+50 Conference, the UN Secretary-General Antonio Guterres construed “climate emergency” as one of the key drivers of the “triple planetary crisis.” Despite this, the overriding impression left by COP27, held in Sharm el-Sheikh in November 2022, was of a divided institution, floundering and nowhere close to realizing its stated aim of “stabilizing greenhouse gas concentrations at a level that would prevent dangerous interferen...
Envisioning Our Environmental Future
With the Stockholm+50 Conference, held on 2-3 June 2022, the global movement to protect the environment has reached a 50 year milestone. The first UN Conference on the Human Environment, also held in Stockholm, from 5-16 June 1972, proved to be the watershed in addressing this problem, and as the world assembles once more in the Swedish capital it is time to think aloud and look ahead. In his address in 1972, the then Swedish Prime Minister Olof Palme said: “The decisive question is in which direction we will develop ... there is no individual future, neither for people nor for nations.” The only other head of government to attend in 1972, Indian Prime Minister Indira Gandhi, highlighted...
